CHO Transfection with LovTAP only

Team:EPF-Lausanne/Protocol/CHO Transfection

We trasfected a batch of CHO cells with LovTAP only. We made two tubes with 100% LovTAP and one with 95% LovTAP and 5% pOri-eGFP (this is a control plasmid with a very high constitutive expression of GFP, it is used to check if the transfection has worked, with the Guava).

Guava Measurements on HEK cells transfected with pHY42 and a GFP readout

Team:EPF-Lausanne/Protocol/Guava-Flowcytometry

A routine measurement of green fluorescence has been performed on the HEK cells transfected with melanopsin. We didn't see a big difference of GFP expression between transfected and non-transfected cells. However, we can notice that the cells that have been under the Arduino blue light for a while have a different shape and less viability.