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Revision as of 17:10, 20 August 2012 (view source) Diego.marcos (Talk | contribs) (→Illumination) ← Older edit		Latest revision as of 18:09, 25 September 2012 (view source) Diego.marcos (Talk | contribs) (Replaced content with "{{:Team:EPF-Lausanne/Template/Header|project}} == Cell culture absorbance test == Raw results in File:Team-EPF-Lausanne-cell-culture-absorbance-nanodrop.pdf. {{:Team:...")
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-	= Illumination =
-	In Strickland's paper, it's mentioned that they used 8000 mcd LEDs from [http://www.theledlight.com theledlight.com], with 20º viewing angle and 468 nm at 3.4 V.	+	== Cell culture absorbance test ==
-	It's also mentioned that a single LED was used per tube, with a cross section of 0.12 cm<sup>2</sup> = 1.2x10<sup>-5</sup> m<sup>2</sup>.	+	Raw results in [[File:Team-EPF-Lausanne-cell-culture-absorbance-nanodrop.pdf]].
-		+
-	20º viewing angle, 0.35 rad, means a solid angle of 2π(1-cos(0.35)) = 0.381 sr.	+
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-	This gives a luminous flux of 0.381*8 = 3.05 lm per LED.	+
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-	The illuminance they used was then 3.05/1.2x10<sup>-5</sup> = 2.54x10<sup>5</sup> lux (or lm/ m<sup>2</sup>).	+
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-	A bioreactor that has some 0.2*π*0.3 = 0.19 m<sup>2</sup>, we would need at least 2.54x10<sup>5</sup> * 0.19 = 48260 lm, or some 16000 LEDs just to make sure the outer surface of the bioreactor has the same light conditions!!	+
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-	This doesn't take into account reflections in the bioreactor, so I hope it will lower this number.	+
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-	== Illuminance test ==	+
-		+
-	We can try first different illuminance values, to get an idea about the range in which the LovTAP-VP16 switch saturates. This could be done by using cylindrical cell culture test plates (there are two sizes: 8.96 sqcm and 3.60 sqcm of circular base).	+
-		+
-	If we take the one with 3.60 sqcm (or 2.14 cm in diameter), considering we have 20º LEDs, the distance between the well bottom and the LED has to be of around 6 cm to have the whole well illuminated.	+
	{{:Team:EPF-Lausanne/Template/Footer}}		{{:Team:EPF-Lausanne/Template/Footer}}

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Latest revision as of 18:09, 25 September 2012