Team:Cornell/testing/project/wetlab/5
From 2012.igem.org
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- | <h3> | + | <h3>Auxotrophy as Means of Selection </h3> |
- | + | Antibiotic resistance genes are powerful tools for molecular biologists, but potentially dangerous if the wrong organisms obtain the resistance genes. One alternative to using antibiotic resistance as a selective pressure to maintain plasmids in bacteria is to engineer bacteria to be auxotrophic for a critical cell metabolite, then re-introduce the genes(s) on the plasmid. One downside to using plasmids is that they are energetically costly to replicate and express genes from and can slow down the overall growth and metabolism and thus electric current output of S. oneidensis. | |
+ | We devoted a lot of time and thought into developing our unique strains of S. oneidensis - another benefit of making our strains auxotrophic is that they will not survive in the wild without our device providing dietary supplements. | ||
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+ | <h3> Chromosomal Integration </h3> | ||
+ | A small plasmid with few expressed genes may not affect the current output of S. oneidensis to a significant degree, but a large plasmid with many expressed genes (such as our secondary naphthalene degradation plasmid) significantly impairs the growth and metabolism of S. oneidensis. Integrating the naphthalene degradation operon into the chromosome of S. oneidensis may help partially alleviate the energy cost of replicating several copies of a huge plasmid. | ||
+ | In addition to alleviating the stress caused by expressing a giant operon, integrating our genetic parts into the chromosome eliminates the need to design a selective pressure for S. oneidensis to maintain extrachromosomal DNA. | ||
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Revision as of 16:54, 2 October 2012