Team:Copenhagen/Project

From 2012.igem.org

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    <li><a href="https://2012.igem.org/Team:Copenhagen/Project/Experimental" CLASS=leftbar>Experimental Design</a></li>
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    <li><a href="https://2012.igem.org/Team:Copenhagen/Protocol" CLASS=leftbar>Protocols</a></li>
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   <font color="#000000" size="7" face="constantia" align="justify"><b>CyanoDelux
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<p align = left><strong>Why cyanobacteria?</strong></p>
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<p align = left><h2>Why cyanobacteria?</h2></p>
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<p>Conventional single celled model organisms, such as E.coli, are widely used in synthetic biology as reliable and well-characterized systems. However a nitrogen and carbon rich medium has to be supplied which is expensive in both economic and environmental terms. Using cyanobacteria capable of performing photosynthesis and removing waste CO2 as well as nitrogen fixating bypasses this problem and has the potential to be an important organism for future biotechnological research. We wish to genetically engineer cyanobacteria to produce biologically generated light, so-called bioluminescence. This not only has direct implications for development of sustainable and economic light sources, but also as a general proof-of-principle of the suitability of cyanobacteria for biotechnological research and production.<a href="https://2012.igem.org/Team:Copenhagen/Project/Cyanobacteria" style="text-decoration:none; color:blue;"> Read more.</a><br></p>
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<p>Conventional single celled model organisms, such as E.coli, are widely used in synthetic biology as reliable and well-characterized systems. However a nitrogen and carbon rich medium has to be supplied which is expensive in both economic and environmental terms. Using cyanobacteria capable of performing photosynthesis and removing waste CO2 as well as nitrogen fixating bypasses this problem and has the potential to be an important organism for future biotechnological research. We wish to genetically engineer cyanobacteria to produce biologically generated light, so-called bioluminescence. This not only has direct implications for development of sustainable and economic light sources, but also as a general proof-of-principle of the suitability of cyanobacteria for biotechnological research and production.<a href="https://2012.igem.org/Team:Copenhagen/Project/Cyanobacteria" style="text-decoration:none; color:blue;"> Read more.</a><br></p></TD>
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<p align = left><strong>General design</strong></p>
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<p align = "left">Initially the project sets with identification of native promoters from cyanobacteria responding to the day-night-cycle. Those will be spliced into an iGEM-biobrick together with the luxCDABE cassette containing the essential luciferase enzyme (and auxillary enzymes necessary for its function). The process is carried out in E.coli, and afterwards the complete plasmid is transferred to cyanobacteria (strain PCC6803). The endogenous transcription factors present in cyanobacteria should appropriately active transcription from the genetically spliced promoter thus driving transcription only during nighttime. In conjunction we will as a proof-of-principle experiment use the already existing plasmids pDawn and pDusk and BioBricks from the iGEM Registry, to, in a similar manner, create a luciferase containing plasmid, but with an artificial promoter system responding directly to light-dark conditions. It serves as a parallel control experiment. Both of these systems will subsequently be thoroughly analyzed to determine important characteristics of the system including kinetics and efficiency of the expression levels. To achieve this quantification we will collaborate with fellow Mathematics and Physics students at University Copenhagen. In this our date can be compared to simulations. As an example it be possible to put CO2 consumption by the cyanobacteria in relation to the emitted bioluminescence thus describing the degree of sustainability or ‘greenness’ of the system.
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<a href="https://2012.igem.org/Team:Copenhagen/Project/Experimental" style="text-decoration:none; color:blue;"> See contructs here</a><br></p>
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<p align = right><strong>Diverse </strong>
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<p>Text her. <a href="text link her" style="text-decoration:none; color:blue;"> Read more.</a><br></p>
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  <strong>Impact factors:</strong>
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  Our project aims to create a regulated light-producing BioBrick and thus add it to the partsregistry of BioBricks, a continuously growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems (partsregistry.org ). Hence our project contributes to the expansion of the partsregistry, benefitting all researchers with interest in bioluminescence in cyanobacterias. The partsregistry is founded on an open source philosophy, a philosophy we see as a great advantage for development and improvement of the number BioBricks already existing. The addition of our BioBrick to this catalogue will perhaps encourage other groups to look at other aspects of the many functions of cyanobacterias and thus contribute to the formation of new biological systems further expanding the field of synthetic biology.</p></p>
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<TD width="600px" height="100%" valign="top"><p align = left><h2>General design</h2></p>
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<p align = "left">Initially the project sets with identification of native promoters from cyanobacteria responding to the day-night-cycle. Those will be spliced into an iGEM-biobrick together with the luxCDABE cassette containing the essential luciferase enzyme (and auxillary enzymes necessary for its function). The process is carried out in E.coli, and afterwards the complete plasmid is transferred to cyanobacteria (strain PCC6803). The endogenous transcription factors present in cyanobacteria should appropriately active transcription from the genetically spliced promoter thus driving transcription only during nighttime. In conjunction we will as a proof-of-principle experiment use the already existing plasmids pDawn and pDusk and BioBricks from the iGEM Registry, to, in a similar manner, create a luciferase containing plasmid, but with an artificial promoter system responding directly to light-dark conditions. It serves as a parallel control experiment. Both of these systems will subsequently be thoroughly analyzed to determine important characteristics of the system including kinetics and efficiency of the expression levels. To achieve this quantification we will collaborate with fellow Mathematics and Physics students at University Copenhagen. In this our date can be compared to simulations. As an example it be possible to put CO2 consumption by the cyanobacteria in relation to the emitted bioluminescence thus describing the degree of sustainability or ‘greenness’ of the system.
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<a href="https://2012.igem.org/Team:Copenhagen/Project/Experimental" style="text-decoration:none; color:blue;"> See full size contructs here.</a><br></p></TD>
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<TD width="600px" height="100%" valign="top"><p align =left><h2>Protocols</h2>
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<p>We don't have any protocols writen yet, but they will be added very soon so visit regularly to get the latest update. <a href="text link her" style="text-decoration:none; color:blue;">See protocols here.</a><br></p></TD>
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<TD width="600px" height="100%" valign="top"><p align = left><h2>Data</h2>
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<p>We don't have any data yet, but it will come soon so visit regularly to get the latest update. <a href="text link her" style="text-decoration:none; color:blue;">See full data page.</a><br></p></TD>
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<TD width="600px" height="100%" valign="top"><p align = left><h2>Results</h2>
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<p>At this point there are no data submitted. Please check later. <a href="text link her" style="text-decoration:none; color:blue;"> See our results.</a><br></p></TD>
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<TD><h2>Impact factors:</h2>
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Our project aims to create a regulated light-producing BioBrick and thus add it to the partsregistry of BioBricks, a continuously growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems (partsregistry.org ). Hence our project contributes to the expansion of the partsregistry, benefitting all researchers with interest in bioluminescence in cyanobacterias. The partsregistry is founded on an open source philosophy, a philosophy we see as a great advantage for development and improvement of the number BioBricks already existing. The addition of our BioBrick to this catalogue will perhaps encourage other groups to look at other aspects of the many functions of cyanobacterias and thus contribute to the formation of new biological systems further expanding the field of synthetic biology.</p></p></TD>
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Revision as of 10:33, 3 August 2012



CyanoDelux

 

We will light your way

 

Why cyanobacteria?

Conventional single celled model organisms, such as E.coli, are widely used in synthetic biology as reliable and well-characterized systems. However a nitrogen and carbon rich medium has to be supplied which is expensive in both economic and environmental terms. Using cyanobacteria capable of performing photosynthesis and removing waste CO2 as well as nitrogen fixating bypasses this problem and has the potential to be an important organism for future biotechnological research. We wish to genetically engineer cyanobacteria to produce biologically generated light, so-called bioluminescence. This not only has direct implications for development of sustainable and economic light sources, but also as a general proof-of-principle of the suitability of cyanobacteria for biotechnological research and production. Read more.

iGEM Medals Requirements


Bronze

Team registration
Complete Judgment form.
Team Wiki.
Plan to present a poster and a talk at the iGEM Jamboree
At least one new submitted and well-characterized standard BioBrick Part or Device.

Silver

Demonstrate that at least one new BioBrick Part of your own design and construction works as expected.
Characterize the operation of at least one new BioBrick Part..


Gold

For Gold Medal, either of the following must be checked

Improve an existing BioBrick Part or Device.
Help another iGEM team
Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation .

General design

Initially the project sets with identification of native promoters from cyanobacteria responding to the day-night-cycle. Those will be spliced into an iGEM-biobrick together with the luxCDABE cassette containing the essential luciferase enzyme (and auxillary enzymes necessary for its function). The process is carried out in E.coli, and afterwards the complete plasmid is transferred to cyanobacteria (strain PCC6803). The endogenous transcription factors present in cyanobacteria should appropriately active transcription from the genetically spliced promoter thus driving transcription only during nighttime. In conjunction we will as a proof-of-principle experiment use the already existing plasmids pDawn and pDusk and BioBricks from the iGEM Registry, to, in a similar manner, create a luciferase containing plasmid, but with an artificial promoter system responding directly to light-dark conditions. It serves as a parallel control experiment. Both of these systems will subsequently be thoroughly analyzed to determine important characteristics of the system including kinetics and efficiency of the expression levels. To achieve this quantification we will collaborate with fellow Mathematics and Physics students at University Copenhagen. In this our date can be compared to simulations. As an example it be possible to put CO2 consumption by the cyanobacteria in relation to the emitted bioluminescence thus describing the degree of sustainability or ‘greenness’ of the system. See full size contructs here.

Protocols

We don't have any protocols writen yet, but they will be added very soon so visit regularly to get the latest update. See protocols here.

Data

We don't have any data yet, but it will come soon so visit regularly to get the latest update. See full data page.

Results

At this point there are no data submitted. Please check later. See our results.

Impact factors:


Our project aims to create a regulated light-producing BioBrick and thus add it to the partsregistry of BioBricks, a continuously growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems (partsregistry.org ). Hence our project contributes to the expansion of the partsregistry, benefitting all researchers with interest in bioluminescence in cyanobacterias. The partsregistry is founded on an open source philosophy, a philosophy we see as a great advantage for development and improvement of the number BioBricks already existing. The addition of our BioBrick to this catalogue will perhaps encourage other groups to look at other aspects of the many functions of cyanobacterias and thus contribute to the formation of new biological systems further expanding the field of synthetic biology.
Comments or questions to the team? Please mail us at igemcopenhagen@gmail.com

Retrieved from "http://2012.igem.org/Team:Copenhagen/Project"