Team:Cambridge/TEST1

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<br> Previous iGEM teams have charaterised an impressive array of inducible promoters, along with other elements of biosensing circuitry... Read More </br>
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<h8 style='color:#339900;'>Human Practices</h8>
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<p><h9>THE DRIVING FORCE</h9>
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<p><h9>Our goal is to standardise the output of biosensors, and to develop a cheap, user-friendly kit to be used in the field</h9>
 +
<p><h9> >>Overview</h9>
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<p><h9> >>Market Research</h9>
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<p><h9> >>Outreach & Collaborations</h9>
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<p><h9> >>Future Directions </h9>
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<h8 style='color:#FFFFFF;'>Parts for a Reliable and Field Ready Biosensing Platform</h8>
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<p><h9>OVERVIEW</h9>
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<p><h9>There are many biosensors available but there is no standard way to deploy them. Many are also non-quantitative and unpredictable. We have been working on a ratiometric luciferase output which can be read by an Arduino device.
 +
This output could be used with theoretically any biosensor. We investigated the potential of riboswitches as future biosensors. We use a B.subtilis chassis as these form low maintenance spores for easy, long-term storage. </h9>
 +
<p><h9> >>Overview</h9>
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<p><h9> >>Design Process</h9>
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<p><h9>A NOVEL FLUORIDE SENSOR BASED ON A RIBOSWITCH CONSTRUCT</h9>
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<p><h9>Riboswitches may well be the biosensor of the future though they are currently under-represented in the registry.</h9>
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<p><a href='/Team:Cambridge/Ribosense/Overview'><h9> >>Overview</h9></a>
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<p><h9>INVESTIGATING THE POTENTIAL OF RATIOMETRIC REPORTER CONSTRUCTS FOR ACCURATE AND REPRODUCIBLE CHARACTERISATION</h9>
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<p><h9>We designed fluorescence and luminescence-based constructs that would function in both E.coli and B.subtilis</h9>
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<p><h9> >>Overview</h9>
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<p><h9> >>Design Process</h9>
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<p><h9> >>Parts</h9>
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<p><h9> >>Lab book</h9>
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<p><h9> >>Results</h9>
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<p><h9>A CHEAP, ARDUINO-BASED, AUTOMATIC ROTARY RATIOLUMINOMETER</h9>
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<p><h9> >>Overview</h9>
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<p><h9> >>Design Process</h9>
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<p><h9> >>Parts</h9>
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<p><h9>B. SUBTILIS: LONG TERM, USER-FRIENDLY STORAGE</h9>
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<p><h9>We developed and optimised procedures for sporulation and germination.</h9>
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<p><h9> >>Overview</h9>
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<p><h9> >>Design Process</h9>
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<br> This section contains a photographic record of our progress during the past two... Read More </br>
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<br> This is a detail record of our work during the Summer... <a href = "https://2012.igem.org/Team:Cambridge/Notebook">Read More </a></br>
 
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<br> Click to view information about the Cambridge team advisors, team members and ... Read More </br>
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<br> Lab safety is always our first priority... Read More </br>
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Latest revision as of 11:06, 25 October 2012

Human Practices

THE DRIVING FORCE

Our goal is to standardise the output of biosensors, and to develop a cheap, user-friendly kit to be used in the field

>>Overview

>>Market Research

>>Outreach & Collaborations

>>Future Directions

Parts for a Reliable and Field Ready Biosensing Platform

OVERVIEW

There are many biosensors available but there is no standard way to deploy them. Many are also non-quantitative and unpredictable. We have been working on a ratiometric luciferase output which can be read by an Arduino device. This output could be used with theoretically any biosensor. We investigated the potential of riboswitches as future biosensors. We use a B.subtilis chassis as these form low maintenance spores for easy, long-term storage.

>>Overview

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RiboSense

A NOVEL FLUORIDE SENSOR BASED ON A RIBOSWITCH CONSTRUCT

Riboswitches may well be the biosensor of the future though they are currently under-represented in the registry.

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Ratiometrica

INVESTIGATING THE POTENTIAL OF RATIOMETRIC REPORTER CONSTRUCTS FOR ACCURATE AND REPRODUCIBLE CHARACTERISATION

We designed fluorescence and luminescence-based constructs that would function in both E.coli and B.subtilis

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Biologger

A CHEAP, ARDUINO-BASED, AUTOMATIC ROTARY RATIOLUMINOMETER

An open-sourced, open-platform approach relaying easily interpretable information to a user that is an accurate representation of the input processed by a biological device.

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Sporage & Distribution

B. SUBTILIS: LONG TERM, USER-FRIENDLY STORAGE

We developed and optimised procedures for sporulation and germination.

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