Team:Cambridge/Protocols

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Contents

Protocols

Safety

See our Safety Page for Associated risk assessments for the protocols below and MSDS sheets for the reagents we have used.

Methods

Construct production

Transformation protocols

Construct verification

Ribosense testing

  • β-galactosidase assay Assay to qualitatively measure the amount of the enzyme β-galactosidase being produced by a population of cells. Useful as a reporter system.
  • Miller assay Assay to quantify the amount of the enzyme β-galactosidase being produced by a population of cells. Useful as a quantitative reporter system.
  • Production of magnesium free cells. Technique to produce cells that contain little or no magnesium, to control for this variable in tests involving the magnesium riboswitch.

Ratiometrica testing

  • IPTG induction A technique for inducing the pSPANK promoter in E.coli cells.

Sporulation and Germination

Miscellaneous

  • BioBricks Resources for manipulation of BioBricks and information regarding the distribution.
  • LB Agar Plates preparation A method used to prepare agar plate to culture common bacteria.
  • Use of Antibiotic Stocks The production of Antibiotic LB agar plates and liquid medium to discriminate bacterial lineages.

Recipes

Lab supplies

  • [http://www.bioc.cam.ac.uk/stores/ BioPath Stores]
  • [http://www.plantsci.cam.ac.uk/camonly/stores/stock.xls Plant Department Stores Catalogue xls (Updated 2nd July 2012)]


Other resources

File:Concentration calculator for lazy scientists.xls

[http://meta.wikimedia.org/wiki/Help:Contents Media wiki editing]

[http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST at NCBI]

[http://www.finnzymes.fi/tm_determination.html finnzymes Tm calculator]

[http://mfold.rna.albany.edu/?q=mfold mfold], DNA or RNA folding forms on the left hand side