Team:British Columbia/NotebookKillSwitches

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British Columbia - 2012.igem.org

Jacob: Odd years

John: Even Years

Previous iGEM Competitions

Team Abstracts: 2011 2010 2009 2008

Parts Registry: Cell Death

Killswitch BioBricks
Numbers Team Year Trigger What it Does
<partinfo>BBa_I716462</partinfo> Arabinose promoter Stops reproduction, no lysis
<partinfo>BBa_K512002</partinfo> Finds GFP on plasmid Eats plasmid? (Jacob says mention "crispr")
<partinfo>BBa_K112808</partinfo>
<partinfo>BBa_K541545</partinfo> Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
<partinfo>BBa_K593009</partinfo> ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
<partinfo>BBa_K628006</partinfo> Protegrin-1 Kill Switch, (Antimicrobial peptide)
Strasbourg 2010 I don't know what the heck.



I don't know what the heck

2011 Tokyo Metropolitan: Killer Bee e.coli?

2011 UNIST Korea: Killed by high and low temps, not if dark.

2010 Harvard: Barnase cleaves RNA!

2010 Hong Kong: Possible new killswitch, not implemented



<partinfo>BBa_K565001</partinfo> <partinfo>BBa_K565002</partinfo> Colicin G,H gene cluster

http://partsregistry.org/wiki/index.php?title=Part:BBa_K565004 Microcin C51 production device

http://partsregistry.org/wiki/index.php?title=Part:BBa_K185004 RelE toxin+Double terminator

Also, check out https://2009.igem.org/Team:UNICAMP-Brazil/Yeastguard. All sorts of kill switches, but may not all work in E. coli.