Team:British Columbia/NotebookKillSwitches

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British Columbia - 2012.igem.org

Jacob: Odd years

John: Even Years

[http://ung.igem.org/Previous_iGEM_Competitions Previous iGEM Competitions]

Team Abstracts: 2011 2010 2009 2008

Parts Registry: [http://partsregistry.org/Cell_death Cell Death]

Killswitch BioBricks
Numbers Team Year Trigger What it Does Where?
<partinfo>BBa_I716462</partinfo> UC-Berkeley 2007 Arabinose promoter BamHI
<partinfo>BBa_K512002</partinfo> USC2011 IPTG, targets GFP gene CRISPR-mediated GFP plasmid degradation
<partinfo>BBa_K112808</partinfo> UC-Berkeley 2008 No PromoterT4 Lysis device Plate 3, 6G
<partinfo>BBa_K541545</partinfo> FAGEM 2011 IPTG Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
<partinfo>BBa_K593009</partinfo> METU-Ankara2011T7 Promoter ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
<partinfo>BBa_K628006</partinfo> St. Andrews 2011 pBad Protegrin-1 Kill Switch, (Antimicrobial peptide)
Strasbourg 2010 I don't know what the heck.
Tokyo Metropolitan 2011 Killer Bee e.coli?
UNIST Korea 2011 Killed by high and low temps, not if dark.
Harvard 2010 Barnase cleaves RNA!
<partinfo>BBa_I716211</partinfo> UC Berkeley 2007 No Promoter Harvard got IPTG inducible from Anderson Lab
Hong Kong 2010 Possible new killswitch, not implemented
<partinfo>BBa_K565001</partinfo> <partinfo>BBa_K565002</partinfo> Colicin G,H gene cluster
<partinfo>BBa_K565004</partinfo> Microcin C51 production device
<partinfo>BBa_K185004</partinfo> RelE toxin+Double terminator
UNICAMP Brazil 2009 All sorts of kill switches, but may not all work in E. coli.
<partinfo>BBa_K302032</partinfo> Design MazF, I think.
<partinfo>BBa_K124014</partinfo> Mutated Holin Gene
<partinfo>BBa_K419003</partinfo> Phage lysis, no antiholin
<partinfo>BBa_K317039</partinfo> AHL sensitive lysis cassette
<partinfo>BBa_K317040</partinfo> IPTG sensitive lysis cassette
<partinfo>BBa_K299806</partinfo> MinC cell division inhibitor
<partinfo>BBa_K150007</partinfo> Colicin E1
<partinfo>BBa_K150010</partinfo> Colicin E9
<partinfo>BBa_K145230</partinfo> LuxR ccdb
<partinfo>BBa_K117009</partinfo> Colicin E7
<partinfo>BBa_K108001</partinfo> Enterobacter phage lysis