Team:British Columbia/NotebookKillSwitches

From 2012.igem.org

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! scope="col" | Trigger
! scope="col" | Trigger
! scope="col" | What it Does
! scope="col" | What it Does
 +
! scope="col" | Where is it?
|-
|-
-
| <partinfo>BBa_I716462</partinfo> || || || Arabinose promoter || Stops reproduction, no lysis
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| <partinfo>BBa_I716462</partinfo> || || || Arabinose promoter || Stops reproduction, no lysis ||
|-
|-
-
| <partinfo>BBa_K512002</partinfo> || || || Finds GFP on plasmid || Eats plasmid? (Jacob says mention "crispr")
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| <partinfo>BBa_K512002</partinfo> || || || Finds GFP on plasmid || Eats plasmid? (Jacob says mention "crispr") ||
|-
|-
-
| <partinfo>BBa_K112808</partinfo> || || || ||
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| <partinfo>BBa_K112808</partinfo> || || || || ||
|-
|-
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| <partinfo>BBa_K541545</partinfo> || || || || Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
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| <partinfo>BBa_K541545</partinfo> || || || || Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible) ||
|-
|-
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| <partinfo>BBa_K593009</partinfo> || || || || ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
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| <partinfo>BBa_K593009</partinfo> || || || || ROSE regulated kill switch, (RNA thermometer, only activates above 42°) ||
|-
|-
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| <partinfo>BBa_K628006</partinfo> || || || || Protegrin-1 Kill Switch, (Antimicrobial peptide)
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| <partinfo>BBa_K628006</partinfo> || || || || Protegrin-1 Kill Switch, (Antimicrobial peptide) ||
|-
|-
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| || Strasbourg || [https://2010.igem.org/Team:ESBS-Strasbourg 2010] || || I don't know what the heck.
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| || Strasbourg || [https://2010.igem.org/Team:ESBS-Strasbourg 2010] || || I don't know what the heck. ||
 +
|-
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| || Tokyo Metropolitan || [https://2011.igem.org/Team:Tokyo_Metropolitan 2011] || || Killer Bee e.coli? ||
 +
|-
 +
| || UNIST Korea || [https://2011.igem.org/Team:UNIST_Korea 2011] || || Killed by high and low temps, not if dark. ||
 +
|-
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| || Harvard || [https://2010.igem.org/Team:Harvard/fences/design 2010] || || Barnase cleaves RNA! ||
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|-
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| || Hong Kong || [https://2010.igem.org/Team:HKU-Hong_Kong/Project 2010] || || Possible new killswitch, not implemented ||
 +
|-
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| <partinfo>BBa_K565001</partinfo> <partinfo>BBa_K565002</partinfo> || || || Colicin G,H gene cluster ||
 +
|-
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| <partinfo>BBa_K565004</partinfo> || || || Microcin C51 production device ||
 +
|-
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| <partinfo>BBa_K185004</partinfo> || || || RelE toxin+Double terminator ||
 +
|-
 +
| || UNICAMP Brazil || [https://2009.igem.org/Team:UNICAMP-Brazil/Yeastguard 2009] || || All sorts of kill switches, but may not all work in E. coli. ||
 +
|-
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| <partinfo>BBa_K302032</partinfo> || || || Design MazF, I think. ||
|}
|}
 +
http://partsregistry.org/wiki/index.php/Part:BBa_K124014
 +
Mutated Holin Gene
 +
http://partsregistry.org/wiki/index.php?title=Part:BBa_K419003
 +
Phage lysis, no antiholin
 +
http://partsregistry.org/wiki/index.php?title=Part:BBa_K317039
 +
AHL sensitive lysis cassette
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: I don't know what the heck
+
http://partsregistry.org/wiki/index.php?title=Part:BBa_K317040
-
 
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IPTG sensitive lysis cassette
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[https://2011.igem.org/Team:Tokyo_Metropolitan 2011 Tokyo Metropolitan]: Killer Bee e.coli?
+
-
[https://2011.igem.org/Team:UNIST_Korea 2011 UNIST Korea]: Killed by high and low temps, not if dark.
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http://partsregistry.org/Part:BBa_K299806
 +
MinC cell division inhibitor
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[https://2010.igem.org/Team:Harvard/fences/design 2010 Harvard]: Barnase cleaves RNA!
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http://partsregistry.org/wiki/index.php?title=Part:BBa_K150007
 +
Colicin E1
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[https://2010.igem.org/Team:HKU-Hong_Kong/Project 2010 Hong Kong]: Possible new killswitch, not implemented
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http://partsregistry.org/wiki/index.php?title=Part:BBa_K150010
 +
Colicin E9
-
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http://partsregistry.org/wiki/index.php?title=Part:BBa_K145230
 +
LuxR ccdb
-
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http://partsregistry.org/wiki/index.php?title=Part:BBa_K117009
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<partinfo>BBa_K565001</partinfo>
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Colicin E7
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<partinfo>BBa_K565002</partinfo>
+
-
Colicin G,H gene cluster
+
-
+
-
http://partsregistry.org/wiki/index.php?title=Part:BBa_K565004
+
-
Microcin C51 production device
+
-
+
-
http://partsregistry.org/wiki/index.php?title=Part:BBa_K185004
+
-
RelE toxin+Double terminator
+
-
Also, check out https://2009.igem.org/Team:UNICAMP-Brazil/Yeastguard. All sorts of kill switches, but may not all work in E. coli.
+
http://partsregistry.org/Part:BBa_K108001
 +
Enterobacter phage lysis

Revision as of 00:43, 31 May 2012

British Columbia - 2012.igem.org

Jacob: Odd years

John: Even Years

Previous iGEM Competitions

Team Abstracts: 2011 2010 2009 2008

Parts Registry: Cell Death

Killswitch BioBricks
Numbers Team Year Trigger What it Does Where is it?
<partinfo>BBa_I716462</partinfo> Arabinose promoter Stops reproduction, no lysis
<partinfo>BBa_K512002</partinfo> Finds GFP on plasmid Eats plasmid? (Jacob says mention "crispr")
<partinfo>BBa_K112808</partinfo>
<partinfo>BBa_K541545</partinfo> Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
<partinfo>BBa_K593009</partinfo> ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
<partinfo>BBa_K628006</partinfo> Protegrin-1 Kill Switch, (Antimicrobial peptide)
Strasbourg 2010 I don't know what the heck.
Tokyo Metropolitan 2011 Killer Bee e.coli?
UNIST Korea 2011 Killed by high and low temps, not if dark.
Harvard 2010 Barnase cleaves RNA!
Hong Kong 2010 Possible new killswitch, not implemented
<partinfo>BBa_K565001</partinfo> <partinfo>BBa_K565002</partinfo> Colicin G,H gene cluster
<partinfo>BBa_K565004</partinfo> Microcin C51 production device
<partinfo>BBa_K185004</partinfo> RelE toxin+Double terminator
UNICAMP Brazil 2009 All sorts of kill switches, but may not all work in E. coli.
<partinfo>BBa_K302032</partinfo> Design MazF, I think.


http://partsregistry.org/wiki/index.php/Part:BBa_K124014 Mutated Holin Gene

http://partsregistry.org/wiki/index.php?title=Part:BBa_K419003 Phage lysis, no antiholin

http://partsregistry.org/wiki/index.php?title=Part:BBa_K317039 AHL sensitive lysis cassette

http://partsregistry.org/wiki/index.php?title=Part:BBa_K317040 IPTG sensitive lysis cassette

http://partsregistry.org/Part:BBa_K299806 MinC cell division inhibitor

http://partsregistry.org/wiki/index.php?title=Part:BBa_K150007 Colicin E1

http://partsregistry.org/wiki/index.php?title=Part:BBa_K150010 Colicin E9

http://partsregistry.org/wiki/index.php?title=Part:BBa_K145230 LuxR ccdb

http://partsregistry.org/wiki/index.php?title=Part:BBa_K117009 Colicin E7

http://partsregistry.org/Part:BBa_K108001 Enterobacter phage lysis

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