Team:British Columbia/NotebookKillSwitches

From 2012.igem.org

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Parts Registry: [http://partsregistry.org/Cell_death Cell Death]
Parts Registry: [http://partsregistry.org/Cell_death Cell Death]
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<partinfo>BBa_I716462</partinfo> Arabinose promoter, stops reproduction, no lysis
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{| border="1"
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|+ Killswitch BioBricks
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! scope="col" | Number
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! scope="col" | Name
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! scope="col" | What it Does
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|-
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| <partinfo>BBa_I716462</partinfo> ||  || Arabinose promoter, stops reproduction, no lysis
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|-
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| <partinfo>BBa_K512002</partinfo> ||  || Finds GFP on plasmid, eats plasmid? (Jacob says mention "crispr")
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|-
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| <partinfo>BBa_K112808</partinfo> ||  ||
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|}
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<partinfo>BBa_K512002</partinfo> Finds GFP on plasmid, eats plasmid? (Jacob says mention "crispr")
 
[https://2010.igem.org/Team:ESBS-Strasbourg 2010 Strasbourg]: I don't know what the heck
[https://2010.igem.org/Team:ESBS-Strasbourg 2010 Strasbourg]: I don't know what the heck
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[https://2010.igem.org/Team:Harvard/fences/design 2010 Harvard]: Barnase cleaves RNA!
[https://2010.igem.org/Team:Harvard/fences/design 2010 Harvard]: Barnase cleaves RNA!
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<partinfo>BBa_K112808</partinfo>
 
[https://2010.igem.org/Team:HKU-Hong_Kong/Project 2010 Hong Kong]: Possible new killswitch, not implemented
[https://2010.igem.org/Team:HKU-Hong_Kong/Project 2010 Hong Kong]: Possible new killswitch, not implemented
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<partinfo>BBa_K541545</partinfo> Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
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<partinfo>BBa_K541545</partinfo>
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Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
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<partinfo>BBa_K593009</partinfo>
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ROSE regulated kill switch
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(RNA thermometer, only activates above 42°)
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<partinfo>BBa_K628006</partinfo>
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Protegrin-1 Kill Switch
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(Antimicrobial peptide)
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<partinfo>BBa_K565001</partinfo>
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<partinfo>BBa_K565002</partinfo>
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Colicin G,H gene cluster
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http://partsregistry.org/wiki/index.php?title=Part:BBa_K565004
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Microcin C51 production device
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http://partsregistry.org/wiki/index.php?title=Part:BBa_K185004
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RelE toxin+Double terminator
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Also, check out https://2009.igem.org/Team:UNICAMP-Brazil/Yeastguard. All sorts of kill switches, but may not all work in E. coli.

Revision as of 19:40, 30 May 2012

British Columbia - 2012.igem.org

Parts Registry: Cell Death

Killswitch BioBricks
Number Name What it Does
<partinfo>BBa_I716462</partinfo> Arabinose promoter, stops reproduction, no lysis
<partinfo>BBa_K512002</partinfo> Finds GFP on plasmid, eats plasmid? (Jacob says mention "crispr")
<partinfo>BBa_K112808</partinfo>



2010 Strasbourg: I don't know what the heck

2011 Tokyo Metropolitan: Killer Bee e.coli?

2011 UNIST Korea: Killed by high and low temps, not if dark.

2010 Harvard: Barnase cleaves RNA!

2010 Hong Kong: Possible new killswitch, not implemented

<partinfo>BBa_K541545</partinfo> Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)

<partinfo>BBa_K593009</partinfo> ROSE regulated kill switch (RNA thermometer, only activates above 42°)

<partinfo>BBa_K628006</partinfo> Protegrin-1 Kill Switch (Antimicrobial peptide)

<partinfo>BBa_K565001</partinfo> <partinfo>BBa_K565002</partinfo> Colicin G,H gene cluster

http://partsregistry.org/wiki/index.php?title=Part:BBa_K565004 Microcin C51 production device

http://partsregistry.org/wiki/index.php?title=Part:BBa_K185004 RelE toxin+Double terminator

Also, check out https://2009.igem.org/Team:UNICAMP-Brazil/Yeastguard. All sorts of kill switches, but may not all work in E. coli.