Team:British Columbia/Interesting Biobricks

From 2012.igem.org

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{{Template:Team:British_Columbia_Header}}
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{| border="1"
{| border="1"
|+ Killswitch BioBricks
|+ Killswitch BioBricks
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! scope="col" | Numbers  
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! Numbers !! Team !! Year !! Trigger !! What it Does !! Where?
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! scope="col" | Team
+
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! scope="col" | Year  
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! scope="col" | Trigger
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! scope="col" | What it Does
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! scope="col" | Where?
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|-
|-
| <partinfo>BBa_I716462</partinfo> ||UC-Berkeley ||2007|| Arabinose promoter || BamHI ||On Plate 2, 3C|
| <partinfo>BBa_I716462</partinfo> ||UC-Berkeley ||2007|| Arabinose promoter || BamHI ||On Plate 2, 3C|
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| <partinfo>BBa_K108001</partinfo> ||Tsinghua ||2008 || ||Enterobacter phage lysis ||
| <partinfo>BBa_K108001</partinfo> ||Tsinghua ||2008 || ||Enterobacter phage lysis ||
|}
|}
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 +
{| border="1"
{| border="1"
|+ Distribution BioBricks
|+ Distribution BioBricks
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! scope="col" | Location
! scope="col" | Part Name  
! scope="col" | Part Name  
! scope="col" | Function
! scope="col" | Function
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! scope="col" | Other
! scope="col" | Other
! scope="col" | Plasmid Resistance
! scope="col" | Plasmid Resistance
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! scope="col" | Finished
 
|-
|-
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| Plate 1|| || || || ||
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| colspan="6" align="center" | Plate 1
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|-
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| 5P|| <partinfo>BBa_P1003</partinfo>|| Kan Resistance|| Resistance|| Full Cassette|| "Kan||  Amp||  Tet"
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| 5P|| <partinfo>BBa_P1003</partinfo>|| Kan Resistance|| Resistance|| Full Cassette|| Kan, Amp, Tet
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|-
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| 7B|| <partinfo>BBa_P1004</partinfo>|| Chlor Resist|| Resistance|| Full Cassette|| "Kan||  Amp"
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| 7B|| <partinfo>BBa_P1004</partinfo>|| Chlor Resist|| Resistance|| Full Cassette|| Kan, Amp
|-
|-
| 14C|| <partinfo>BBa_C0261</partinfo>|| AHL Synthase|| AHL Kill Switch|| No Promoter || Amp
| 14C|| <partinfo>BBa_C0261</partinfo>|| AHL Synthase|| AHL Kill Switch|| No Promoter || Amp
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| 24E|| <partinfo>BBa_I13600</partinfo>|| CFP reporter|| fluorescence || 0|| Amp
| 24E|| <partinfo>BBa_I13600</partinfo>|| CFP reporter|| fluorescence || 0|| Amp
|-
|-
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| Plate 2|| || || || ||
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| colspan="6" align="center" | Plate 2
|-
|-
| 17F|| <partinfo>BBa_I20260</partinfo>|| GFP reporter|| Fluorescence || Strong const.|| Kan
| 17F|| <partinfo>BBa_I20260</partinfo>|| GFP reporter|| Fluorescence || Strong const.|| Kan
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| 9B|| <partinfo>BBa_J09250</partinfo>|| GFP reporter|| Fluoresence|| Strong Const.|| Kan
| 9B|| <partinfo>BBa_J09250</partinfo>|| GFP reporter|| Fluoresence|| Strong Const.|| Kan
|-
|-
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| Plate 3|| || || || ||
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| colspan="6" align="center" | Plate 3
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| 2D|| <partinfo>BBa_I746909</partinfo>|| Superfold GFP|| Fluoresence|| T7|| Amp
| 2D|| <partinfo>BBa_I746909</partinfo>|| Superfold GFP|| Fluoresence|| T7|| Amp
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| 13M|| <partinfo>BBa_K093012</partinfo>|| RFP|| Fluorescence|| Strong const.|| Amp
| 13M|| <partinfo>BBa_K093012</partinfo>|| RFP|| Fluorescence|| Strong const.|| Amp
|-
|-
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| 14E|| <partinfo>BBa_K081018</partinfo>|| TetR|| Resistance|| Strong const.|| "Amp||  Kan"
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| 14E|| <partinfo>BBa_K081018</partinfo>|| TetR|| Resistance|| Strong const.|| Amp, Kan
|-
|-
| 15B|| <partinfo>BBa_K112000</partinfo>|| Holin|| Kill Switch|| No promoter|| ?Spec
| 15B|| <partinfo>BBa_K112000</partinfo>|| Holin|| Kill Switch|| No promoter|| ?Spec
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| 20I|| <partinfo>BBa_K124003</partinfo>|| Lysis Cassette|| Kill Switch|| ?|| Kan
| 20I|| <partinfo>BBa_K124003</partinfo>|| Lysis Cassette|| Kill Switch|| ?|| Kan
|-
|-
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| 20K|| <partinfo>BBa_K124014</partinfo>|| M S Holin|| Kill Switch|| No Promoter|| "Amp||  Kan"
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| 20K|| <partinfo>BBa_K124014</partinfo>|| M S Holin|| Kill Switch|| No Promoter|| Amp, Kan
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| 20M|| <partinfo>BBa_K124017</partinfo>|| M Lysis cassette|| Kill Switch|| No Promoter|| Kan
| 20M|| <partinfo>BBa_K124017</partinfo>|| M Lysis cassette|| Kill Switch|| No Promoter|| Kan
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| 21O|| <partinfo>BBa_K106674</partinfo>|| GFP|| Fluorescence|| Med. Const.|| ?amp
| 21O|| <partinfo>BBa_K106674</partinfo>|| GFP|| Fluorescence|| Med. Const.|| ?amp
|-
|-
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| 22A|| <partinfo>BBa_K131000</partinfo>|| Colicin E2|| Kill Switch|| SOS promoter|| "Kan||  Amp"
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| 22A|| <partinfo>BBa_K131000</partinfo>|| Colicin E2|| Kill Switch|| SOS promoter|| Kan, Amp
|-
|-
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| 22C|| <partinfo>BBa_K131010</partinfo>|| Colicin E2|| Kill Switch|| "AHL induce,  c GFP"|| "Kan||  Amp"
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| 22C|| <partinfo>BBa_K131010</partinfo>|| Colicin E2|| Kill Switch|| AHL induce,  c GFP || "Kan, Amp
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|-
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| 24E|| <partinfo>BBa_K112022</partinfo>|| Lysis Device || Kill Switch|| "No Prom, weak anti protein"|| "?Amp||  Kan"
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| 24E|| <partinfo>BBa_K112022</partinfo>|| Lysis Device || Kill Switch|| No Prom, weak anti protein || Amp, Kan
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|-
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| Plate 4|| || || || ||
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| colspan="6" align="center" | Plate 4
|-
|-
| 3I|| <partinfo>BBa_K398406</partinfo>|| Solvent Tolerant|| Other (Hex. Tol)|| Strong const.|| Chlor
| 3I|| <partinfo>BBa_K398406</partinfo>|| Solvent Tolerant|| Other (Hex. Tol)|| Strong const.|| Chlor
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| 21G|| <partinfo>BBa_K314203</partinfo>|| Tse Tox. Ind.Anti|| Kill switch|| HSL ind immunity|| Chlor
| 21G|| <partinfo>BBa_K314203</partinfo>|| Tse Tox. Ind.Anti|| Kill switch|| HSL ind immunity|| Chlor
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|-
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| Plate 5|| || || || ||
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| colspan="6" align="center" | Plate 5
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| 1A|| <partinfo>BBa_K515107</partinfo>|| Photo-Switch|| Death test/Fluor|| pTet|| Chlor
| 1A|| <partinfo>BBa_K515107</partinfo>|| Photo-Switch|| Death test/Fluor|| pTet|| Chlor

Latest revision as of 04:05, 16 June 2012

British Columbia - 2012.igem.org

Killswitch BioBricks
Numbers Team Year Trigger What it Does Where?
<partinfo>BBa_I716462</partinfo> UC-Berkeley 2007 Arabinose promoter BamHI
<partinfo>BBa_K512002</partinfo> USC2011 IPTG, targets GFP gene CRISPR-mediated GFP plasmid degradation
<partinfo>BBa_K112808</partinfo> UC-Berkeley 2008 No PromoterT4 Lysis device Plate 3, 6G
<partinfo>BBa_K541545</partinfo> FAGEM 2011 IPTG Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
<partinfo>BBa_K593009</partinfo> METU-Ankara2011T7 Promoter ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
<partinfo>BBa_K628006</partinfo> St. Andrews 2011 pBad Protegrin-1 Kill Switch, (Antimicrobial peptide)
Strasbourg 2010 I don't know what the heck.
Tokyo Metropolitan 2011 Killer Bee e.coli?
UNIST Korea 2011 Killed by high and low temps, not if dark.
Harvard 2010 Barnase cleaves RNA!
<partinfo>BBa_I716211</partinfo> UC Berkeley 2007 No Promoter Harvard got IPTG inducible Barnase/Barnstar from Anderson Lab at UCB
Hong Kong 2010 Possible new killswitch, not implemented
<partinfo>BBa_K565001</partinfo> Valencia2011 ? 12 kb Colicin G
<partinfo>BBa_K565002</partinfo> Valencia 2011 ? 12 kb Colicin H
<partinfo>BBa_K565004</partinfo> Valencia 2011 No Promoter Microcin C51 production device (5 restriction sites)
<partinfo>BBa_K185004</partinfo> SJTU-BioX-Shanghai 2009 No Promoter RelE toxin+Double terminator
UNICAMP Brazil 2009 All sorts of kill switches, but may not all work in E. coli.
<partinfo>BBa_K284017</partinfo> UNICAMP-Brazil 2009 Lactate (Yeast) Lysozyme
<partinfo>BBa_K131000</partinfo> Calgary2008 SOS Promoter Colicin 2E3, 22A
<partinfo>BBa_K112806</partinfo> UC Berkeley 2008 T7 T4 Endolysin
<partinfo>BBa_K302035</partinfo> Newcastle 2010 Sucrose limit MazEF
<partinfo>BBa_K124014</partinfo> Brown 2008 No Promoter Mutated Holin Gene (Inconsistent sequencing)
<partinfo>BBa_K419003</partinfo> TzuChiU_Formosa 2010 pLysLysis Cassette - no antiholin
<partinfo>BBa_K317039</partinfo> Tokyo-NoKoGen 2010AHL AHL sensitive lysis cassette
<partinfo>BBa_K317040</partinfo> Tokyo-NoKoGen 2010 IPTG IPTG sensitive lysis cassette
<partinfo>BBa_K299806</partinfo> Warsaw 2010 MinC cell division inhibitor
<partinfo>BBa_K150007</partinfo> Heidelberg2008 Native Operon Colicin E1
<partinfo>BBa_K150010</partinfo> Heidelberg2008 Native Operon Colicin E9
<partinfo>BBa_K145230</partinfo> KULeuven 2008 HSL-LuxR ccdb
<partinfo>BBa_K117009</partinfo> NTU-Singapore 2008 IPTG Colicin E7
<partinfo>BBa_K117000</partinfo> NTU-Singapore 2008 Lysin (activates Colicin E7)
<partinfo>BBa_K108001</partinfo> Tsinghua 2008 Enterobacter phage lysis


Distribution BioBricks
Location Part Name Function Use Other Plasmid Resistance
Plate 1
5P <partinfo>BBa_P1003</partinfo> Kan Resistance Resistance Full Cassette Kan, Amp, Tet
7B <partinfo>BBa_P1004</partinfo> Chlor Resist Resistance Full Cassette Kan, Amp
14C <partinfo>BBa_C0261</partinfo> AHL Synthase AHL Kill Switch No Promoter Amp
24E <partinfo>BBa_I13600</partinfo> CFP reporter fluorescence 0 Amp
Plate 2
17F <partinfo>BBa_I20260</partinfo> GFP reporter Fluorescence Strong const. Kan
14N <partinfo>BBa_K117009</partinfo> Colicin E7 Kill Switch Lactose prom Amp
14D <partinfo>BBa_K117000</partinfo> Lysis gene Kill Switch Aids colicin Amp
9B <partinfo>BBa_J09250</partinfo> GFP reporter Fluoresence Strong Const. Kan
Plate 3
2D <partinfo>BBa_I746909</partinfo> Superfold GFP Fluoresence T7 Amp
5G <partinfo>BBa_K137076</partinfo> H2O2 gen. Kill Switch AHL induce Amp
6G <partinfo>BBa_K112808</partinfo> Lysis Device Kill Switch No Promoter Amp
13M <partinfo>BBa_K093012</partinfo> RFP Fluorescence Strong const. Amp
14E <partinfo>BBa_K081018</partinfo> TetR Resistance Strong const. Amp, Kan
15B <partinfo>BBa_K112000</partinfo> Holin Kill Switch No promoter ?Spec
15D <partinfo>BBa_K112005</partinfo> "RBS, Holin" Kill Switch No Promoter ?Spec
15H <partinfo>BBa_k112017</partinfo> lysozyme Kill Switch No Promoter ?Spec
15J <partinfo>BBa_K112106</partinfo> BgIII endonuc. Kill Switch No Promoter ?Spec
20I <partinfo>BBa_K124003</partinfo> Lysis Cassette Kill Switch ? Kan
20K <partinfo>BBa_K124014</partinfo> M S Holin Kill Switch No Promoter Amp, Kan
20M <partinfo>BBa_K124017</partinfo> M Lysis cassette Kill Switch No Promoter Kan
21O <partinfo>BBa_K106674</partinfo> GFP Fluorescence Med. Const. ?amp
22A <partinfo>BBa_K131000</partinfo> Colicin E2 Kill Switch SOS promoter Kan, Amp
22C <partinfo>BBa_K131010</partinfo> Colicin E2 Kill Switch AHL induce, c GFP "Kan, Amp
24E <partinfo>BBa_K112022</partinfo> Lysis Device Kill Switch No Prom, weak anti protein Amp, Kan
Plate 4
3I <partinfo>BBa_K398406</partinfo> Solvent Tolerant Other (Hex. Tol) Strong const. Chlor
6M <partinfo>BBa_K112806</partinfo> T4 Endolysin Kill Switch No Prom Amp
7C <partinfo>BBa_K314200</partinfo> Tse Toxin Kill Switch No Prom Chlor
8G <partinfo>BBa_K206000</partinfo> pBAD Promoter Promoter Arabinose ind Amp
21G <partinfo>BBa_K314203</partinfo> Tse Tox. Ind.Anti Kill switch HSL ind immunity Chlor
Plate 5
1A <partinfo>BBa_K515107</partinfo> Photo-Switch Death test/Fluor pTet Chlor
12O <partinfo>BBa_I13604</partinfo> Con. YFP ind CFP Fluor Strong const./lacI Amp
14I <partinfo>BBa_K299806</partinfo> minC Kill Switch No Promoter Amp