Team:Amsterdam/ernst/
From 2012.igem.org
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- | Prokaryotes have been selected through evolutionary processes for accurate sensing | + | <h1>Neeeee</h1> |
+ | <p>A Prokaryotes have been selected through evolutionary processes for accurate sensing | ||
and acting upon their living environments. This bacterial versatility can be used by | and acting upon their living environments. This bacterial versatility can be used by | ||
us, humans, to sense the environments in places we have trouble reaching. Maybe we | us, humans, to sense the environments in places we have trouble reaching. Maybe we | ||
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maintenance in order to get an idea of the time-variation of the studied system. Could | maintenance in order to get an idea of the time-variation of the studied system. Could | ||
we make the cell ‘remember’ what it has sensed and when so we can leave it alone for a | we make the cell ‘remember’ what it has sensed and when so we can leave it alone for a | ||
- | while and make it report back to us later?< | + | while and make it report back to us later?</p> |
- | + | <p>Meet E. memo, a ‘cellular logbook’, which uses the naturally occurring phenomenon | |
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- | Meet E. memo, a ‘cellular logbook’, which uses the naturally occurring phenomenon | + | |
of <b>DNA methylation</b> to robustly store signals it has sensed in its environment. The | of <b>DNA methylation</b> to robustly store signals it has sensed in its environment. The | ||
Amsterdam iGEM 2012 team, consisting of six students, will dedicate the summer to | Amsterdam iGEM 2012 team, consisting of six students, will dedicate the summer to | ||
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could be linked to any of the many biological sensors that are available in the DNA | could be linked to any of the many biological sensors that are available in the DNA | ||
parts registry. E. memo therefore holds great promise as a detect & store–system for | parts registry. E. memo therefore holds great promise as a detect & store–system for | ||
- | experimental and industrial purposes.< | + | experimental and industrial purposes.</p> |
- | + | <p>Just storing whether certain signals have been sensed by the cell is only half of the story | |
- | Just storing whether certain signals have been sensed by the cell is only half of the story | + | |
however. The proposed memory mechanism would be a form of volatile memory, | however. The proposed memory mechanism would be a form of volatile memory, | ||
of which the traces slowly dissappear as the E. memo-population keeps proliferating. | of which the traces slowly dissappear as the E. memo-population keeps proliferating. | ||
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when a signal has been sensed from the percentage of bits that is methylated, which | when a signal has been sensed from the percentage of bits that is methylated, which | ||
slowly decreases as the cells keep proliferating. This way, we won’t just store whether | slowly decreases as the cells keep proliferating. This way, we won’t just store whether | ||
- | a certain signal has occured; we will also know when it happened.< | + | a certain signal has occured; we will also know when it happened.</p> |
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+ | <h2>Molecular mechanism</h2> | ||
In short, we will introduce a site-specific methyltransferase into the iGEM default | In short, we will introduce a site-specific methyltransferase into the iGEM default | ||
chassis organism E. coli, that will only be active/transcribed when the measured signal | chassis organism E. coli, that will only be active/transcribed when the measured signal | ||
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the span of time we can use to do measurements on.</p> | the span of time we can use to do measurements on.</p> | ||
</div> | </div> | ||
+ | </div> | ||
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</html> | </html> | ||
{{Team:Amsterdam/ernst/Foot}} | {{Team:Amsterdam/ernst/Foot}} |
Latest revision as of 12:40, 4 September 2012