Molecular mechanism

In short, we will introduce a site-specific methyltransferase into the iGEM default chassis organism E. coli, that will only be active/transcribed when the measured signal is encountered by the logbook-cell. The activated methyltransferase will then move over to a plasmid region we’ve termed the bit and append a methyl-group to it. By 1 linking the methyltransferase to a Zinc-Finger, its site-specificity is greatly increased, reducing the amount of undesired background methylation events to a minimum. Furthermore, by slowing down the cell replication cycle of the cells, we can increase the span of time we can use to do measurements on.