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• Jamboree/Team Abstracts
  ...m is adaptable to sensing a wide range of analytes, we have focused on the detection of arsenic-containing compounds and naphthalene, which are common contamina ====[[Team:Gaston Day School | Team Gaston Day School]]: Detection of Heavy Metal Contaminants in Water====
  173 KB (25,944 words) - 17:38, 29 September 2013
• Team:TU Munich/Notebook/Labjournal
  '''Detection:''' * To test wheather the increased protein concentrations lead to overloaded bonds, two SDS-PAGE gels which have been stained with Coomass
  793 KB (114,680 words) - 14:38, 3 May 2013
• Team:TU Munich/Notebook/Meetings
  ...third field of research. This module is promising, because it is likely to lead to easy successes (only one gene required for the production of thaumatin.. ...tibody! A search engine exists as well…does anybody know the name???) or detection via tag (strep-tag or his-tag)
  103 KB (15,460 words) - 13:23, 26 September 2012
• Team:TU Munich/Project/Limonene
  ...oved expression and carries a C-Terminal ''Strep''-Tag for purification or detection by westernblot. It is an improved version of BBa_I742111. Our in vivo analysis strongly indicates that the consensus sequence does lead to a 2-3-fold enhanced expression in yeast, though (see Fig. 8B). This is c
  26 KB (3,889 words) - 01:18, 27 October 2012
• Team:Bielefeld-Germany/Project/Background
  ...drinking water. Consequently the increasing consumption of pharmaceuticals lead to an increasing pressure on the aquatic environment and on the availabilit UV-light detection: 230 nm Extinction, 310 nm Emission <br>
  36 KB (5,073 words) - 00:21, 27 October 2012
• Team:Gaston Day School/Project
  ...f the detector could potentially save lives around the world through early detection of the contamination. ... GFP (BBa_I13401). However, the [http://partsregistry.org/Part:BBa_I721001 lead responsive promoter] was not known to function, according to the [http://op
  4 KB (683 words) - 20:55, 3 October 2012
• Team:Amsterdam/project/features and applications
  ...patterns to the newly synthesized strand during DNA replication. This will lead to a dilution of the amount of ‘written’-plasmids over time, mostly due On and off detection of a signal's presence is useful, but often in biology gaining insight in t
  10 KB (1,634 words) - 03:52, 27 September 2012
• Team:UIUC-Illinois/Notebook/MeetingNotes
  survey- Anthony will lead first JC this week of 2/26 o Whoever will lead JC meeting of the week will create a small power point outlining the paper
  158 KB (27,926 words) - 19:24, 2 October 2012
• Team:Fatih-Medical/Future Work
  ...st probably, are able to bind EpCAM strictly. This makes us think that the detection of CTCs as a result of binding to an EpCAM antigen with C215 antibody is ac ... specific markers are discovered. It should be considered that the current detection techniques are expensive and time-consuming; but, a modified organism with
  7 KB (1,098 words) - 02:54, 27 September 2012
• Team:ETH Zurich/Project overview
  ...tc. <span class='eth_reference'>[Sinha2002]</span> If unattended, this can lead to sun burns, skin damage or even cancer. Thus, a proper skin protection is === Direct UV detection: a novel UV-B light receptor in ''E.coli''===
  7 KB (1,155 words) - 02:14, 27 October 2012
• Team:Potsdam Bioware/Project/Part Antibody
  ...s construct, the work object of our selection module. This interaction can lead to selective collection of a mutated and thus optimized antibody. ...amino acids (DYKDE) that allows us a handy purification and simplifies its detection. A TEV protease cleavage site was integrated between the scFv and the trans
  28 KB (4,268 words) - 18:41, 26 October 2012
• Team:Amsterdam/project/applications/
  ...patterns to the newly synthesized strand during DNA replication. This will lead to a dilution of the amount of ‘written’-plasmids over time, mostly due <h4>Clean Water Supply Detection</h4>
  8 KB (1,321 words) - 05:36, 25 September 2012
• Team:Wageningen UR/MethodsDetection
  = Detection of Virus Like Particles = A key part of our project is the detection of [[Team:Team:Wageningen_UR/VLPs|VLPs]]. We need sufficient visualization
  20 KB (3,208 words) - 16:45, 26 October 2012
• Team:Amsterdam/project/applications/main applications
  ...patterns to the newly synthesized strand during DNA replication. This will lead to a dilution of the amount of ‘written’-plasmids over time, mostly due <h4>Clean Water Supply Detection</h4>
  8 KB (1,300 words) - 14:49, 24 September 2012
• Team:Amsterdam/modeling/odemodel
  Using the here presented model, we will examine how to infer the signal detection time from the amount of methylated plasmids. The cellular division rate det ...patterns to the newly synthesized strand during DNA replication. This will lead to a dilution of the amount of ‘written’-plasmids over time, mostly due
  13 KB (2,106 words) - 07:16, 24 September 2012
• Team:Amsterdam/achievements/time inference model
  Using the here presented model, we will examine how to infer the signal detection time from the amount of methylated plasmids. The cellular division rate det ...patterns to the newly synthesized strand during DNA replication. This will lead to a dilution of the amount of ‘written’-plasmids over time, mostly due
  13 KB (2,121 words) - 17:51, 26 September 2012
• Team:Amsterdam/data/time inference model
  Using the here presented model, we will examine how to infer the signal detection time from the amount of methylated plasmids. The cellular division rate det ...patterns to the newly synthesized strand during DNA replication. This will lead to a dilution of the amount of ‘written’-plasmids over time, mostly due
  13 KB (2,121 words) - 03:53, 27 September 2012
• Team:Stanford-Brown/VenusLife/Chamber
  ... excitation wavelength and light filters on the camera. For bioluminescent detection, the chamber could be equipped with a charge-coupled-device (CCD) camera, o ...cells themselves. The tunability of the signal can be paired with existing detection techniques as well, such as bioluminescence and fluorescence.
  7 KB (990 words) - 07:59, 27 November 2012
• Team:ETH Zurich/Motivation
  ... of the major suspects in provoking DNA alteration in melanocytes that can lead to melanoma. ...ses of malignant melanoma are diagnosed worldwide, of that 20-25 % finally lead to the death of the patient, making the malignant melanoma one of the most
  3 KB (489 words) - 18:50, 26 October 2012
• Team:Chalmers-Gothenburg/Results
  ...anMX] but in neither strains could the receptor be proved as functional in detection of hCG. ...ast with these fragments, homologous recombination should occur which will lead to the exchange of the ''CWP2'' gene with the kanMX cassette.
  50 KB (8,240 words) - 21:01, 26 September 2012
• Team:Calgary/Project/FRED/Detecting
  TITLE=A Transposon-Mediated Mutant Library for Toxin Detection| ...es to efficiently isolate the genetic elements that detect and potentially lead to the breakdown of these toxins.
  14 KB (2,260 words) - 03:10, 27 October 2012
• Team:University College London/Module 1/Design
  =Module 1: Detection= ...://static.igem.org/mediawiki/2012/e/ef/UcligemDetection_BioBrick.png" alt="Detection" /></div></html>
  3 KB (436 words) - 17:11, 26 September 2012
• Team:Groningen/in development
  ...ring errors during the production of an eventual Food Warden product could lead to issues with the germination of the spores, ...is positive control pigment should of course not interfere with the visual detection of the warning pigment. To ensure this,
  19 KB (2,633 words) - 23:58, 26 October 2012
• Team:Calgary/Notebook/Hydrocarbon
  ...e abiotic standards also largely decreased by about the same amount. This lead us to conclude that the reason for decrease was not due to the bacteria met ...rforming a PCR purification. Again, the gel purification procedure did not lead to successful purification of our target gene. Due to the problems we have
  101 KB (16,854 words) - 18:44, 21 September 2012
• Team:Bielefeld-Germany/Labjournal
  * the detection of multiresistent pathogens ...9002045 A disposable Laccase–Tyrosinase based biosensor for amperometric detection of phenolic compounds in must and wine]
  333 KB (50,931 words) - 03:59, 27 October 2012
• Team:NTNU Trondheim/Notebook
  ...B-lactamase in old culture media may degrade ampicillin at a high rate and lead to decreased plasmid stability, also when a new culture is inoculated using ...have three different modules involved in our project; a production part, a detection part and a lysis part. We decided to split in three groups and investigate
  95 KB (15,220 words) - 17:15, 25 September 2012
• Team:Grenoble/Modeling/Conclusion
  ...In the signaling part, we saw that when the dipeptide is detected, it will lead to the production of adenylate cyclase. Then, when the adenylate cyclase is ...given in the quorum sensing part = 10<SUP>-15</SUP> L), we obtain that our detection threshold is around 20 molecules.
  3 KB (563 words) - 16:13, 26 September 2012
• Team:Grenoble/Project/Overview
  <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#10">detection</a>, <h3>Detection:</h3>
  7 KB (1,034 words) - 16:52, 8 March 2013
• Team:NTNU Trondheim/Test/Notebook
  ...B-lactamase in old culture media may degrade ampicillin at a high rate and lead to decreased plasmid stability, also when a new culture is inoculated using ...have three different modules involved in our project; a production part, a detection part and a lysis part. We decided to split in three groups and investigate
  95 KB (15,213 words) - 09:43, 21 September 2012
• Team:Bielefeld-Germany/Protocols/Analytics
  *Connect the power lead and run the stacking gel with 10 mA until the blue dye front enters the sep * Fluorescence-detection
  17 KB (2,614 words) - 22:30, 26 October 2012
• Team:HKUST-Hong Kong/Potential Application
  ... challenges, as reflected by the low detecting rate. Primarily, the first detection of colon cancer is either depending on stool tests or endoscopic screening ...se of the simplest therapeutic access, orally taking medicine, to perform detection. In the meanwhile, it combines the function of a cancer detector and comba
  14 KB (1,834 words) - 14:29, 25 September 2012
• Team:WHU-China/Notebooks
  title : 'Cellulose Detection', href : '#Cellulose Detection'
  104 KB (15,269 words) - 04:04, 27 October 2012
• Team:TU-Delft/HP/DocTalk
  ... future. He gave a couple of examples that would be improved with this new detection method, those are listed below. <br/> ...e patients do not have unfortunately. If we would have a broad platform of detection many different gaseous compounds, the diagnosing of the unknown infections
  4 KB (687 words) - 14:23, 14 November 2012
• Team:UNITN-Trento/Project
  <li>Lead acetate strips</li> ...pher (MICROGC A3000 Agilent) equipped with a 50C Poraplot U column for the detection of H₂S. Damiano keeps his H₂S stock in 10 L champagne
  64 KB (9,720 words) - 22:48, 26 October 2012
• Team:NRP-UEA-Norwich/Future
  ...sensors exist, but usually have problems with specific and quantitative NO detection (Bedioui, F .et al. 2002). To do this in a more specific manner, a comparat ... bacterial species that compose soil flora produce and reduce nitric oxide lead to different levels of NO in soil, which can affect soil fertility and thus
  12 KB (1,915 words) - 23:41, 26 September 2012
• Team:UNITN-Trento/Project/CrustAway
  <li>Lead acetate strips</li> ...pher (MICROGC A3000 Agilent) equipped with a 50C Poraplot U column for the detection of H₂S. Damiano keeps his H₂S stock in 10 L champagne
  64 KB (9,720 words) - 22:47, 26 October 2012
• Team:Calgary/Notebook/Denitrogenation
  ...rforming a PCR purification. Again, the gel purification procedure did not lead to successful purification of our target gene. Due to the problems we have ... to measure bacterial growth and had an ammonia assay performed on it. The detection of ammonia acted as a proxy for detecting carbazole degradation as it is an
  41 KB (7,115 words) - 00:16, 27 October 2012
• Team:Slovenia/ImplementationHepatitisC
  <p>We estimated, based on the detection of IFN-α produced by HEK293T cells, that sufficient quantities of the ther ...ses lead to severe behavioral or psychological disorders. This can in turn lead to the discontinuation of therapy.</p>
  33 KB (4,616 words) - 20:46, 26 October 2012
• Team:Potsdam Bioware/Project/At a Glance
  ...10⁶ can be achieved, which is a good starting point to identify lead compounds for binding. However, to generate high affinity antibodies, furth ...rate cells that transport enough molecules to their surface for successful detection. However we have shown that the switch from presenting antibodies to secret
  23 KB (3,610 words) - 03:54, 27 October 2012
• Team:Austin Texas/Spinach reporter
  ...rious fluorescent plate readers, often wasting whole trials due to machine detection error.The promoter that would be tested for Spinach fluorescence was the T7 ...ere grown in a shaking incubator and then measured in a plate reader. This lead to the hypothesis that low oxygen conditions that existed in the machine du
  9 KB (1,413 words) - 03:58, 4 October 2012
• Team:WHU-China/Modeling
  title : 'Cellulose Detection', href : '#Cellulose Detection'
  60 KB (8,621 words) - 03:54, 27 October 2012
• Team:OUC-China
  <p>Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the inp ...A(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between
  20 KB (2,699 words) - 13:25, 26 October 2012
• Team:Gaston Day School
  <h1><center>Detection of Heavy Metal Contaminants in Water</h1><br> ... last year’s nitrate detector in order to focus on cadmium, arsenic, and lead contaminants in water. These metals are known to be used in insecticides, f
  6 KB (896 words) - 21:13, 3 October 2012
• Team:Grenoble/Biology/Protocols/Gel
  Using parts from another type of tank may lead to the loss of safety protections. We managed to assign a The detection does not let the device generate electricity until the migration buffer has
  4 KB (557 words) - 08:20, 25 September 2012
• Team:Chalmers-Gothenburg/Biodetection of hCG
  yeast. Homologous recombination will lead to the exchange of ''CWP2'' with kanMX in the yeast's genome. ...anMX] but in neither strains could the receptor be proved as functional in detection of hCG.
  13 KB (2,003 words) - 20:59, 26 September 2012
• Team:JUIT-India/Project
  ...biology tools to insert two genes into a bacterial cassette along with its detection systems. This nitrate, as we know, can in turn, be utilized by the plant it ...sive use of fertilizers. The microbes naturally present in the paddy field lead to the emission of nitrous oxide by utilizing these chemical fertilizers. .
  12 KB (1,774 words) - 18:48, 26 September 2012
• Team:Colombia/Safety
  ... cause irritation of mucous membrane and upper respiratory track but never lead to death. ...lor markers and even more importantly we hope to build our bacterial-based detection system on organisms representing low-to-no risk for the normal microbiota o
  14 KB (2,161 words) - 00:17, 27 September 2012
• Team:Wageningen UR/ProjectDescription
  ... the outside of the particle. The research done on these three VLPs should lead to one standardized tool. The formation of the first VLP samples is confirmed by using these two detection methods, and the gene is standardized conform the rules of iGEM. This gene
  2 KB (367 words) - 10:48, 21 September 2012
• Team:Virginia/Modeling
  ... pertussis is the lack of a cheap, fast and reliable method. Our method of detection, where phages are used to indicate the presence of pathogens, fix these sho ...nd outflow of population at the rates shown on the arrows. An inflow would lead a positive term in the ODE for the compartment, while an outflow would be r
  17 KB (2,435 words) - 02:24, 27 October 2012
• Team:TU-Eindhoven/LEC/Lab
  ...fferent GECO proteins at last. To make sure that the transformations would lead to the desired strain, it was decided to start the first step with transfor <h3>Detection of GECO protein in yeast</h3>
  11 KB (1,751 words) - 02:17, 27 September 2012
• Team:Goettingen/Project/Methods
  ...ix. Thus, the absorption spectrum is biased so that it is suitable for DNA detection. The determination of separated molecule sizes is done accodrding ... strong anionic detergent, binds to hydrophobic amino acid residues, which lead the proteins become negatively charged relative to their mass (~1.4 g SDS/g
  29 KB (4,601 words) - 18:05, 26 September 2012
• Team:Cambridge/Project/MagnesiumRiboswitch
  ...n was upstream of sfGFP, consequently we hope that expression of LacI will lead to repression of sfGFP. ...uct.png|center|700px|thumb|The construct made in the pJS130 vector for the detection of changes in Mg2+ ion concentrations]]
  4 KB (560 words) - 16:51, 26 October 2012
• Team:Wageningen UR/InsideModification
  ...wing this we can assume that the fusion of a K-coil to the C-terminal will lead to the formation of a VLP with a coil presented on the inside (figure 14). ...R/MethodsDetection#Detection_of_Virus_Like_Particles" title="Detection">6. Detection</a>
  13 KB (2,166 words) - 20:52, 26 October 2012
• Team:Wageningen UR/Applications
  ...s, we make them available for future optimizations and uses, to ultimately lead to a limitless range of applications. It is our hope that one day, this ran ...R/MethodsDetection#Detection_of_Virus_Like_Particles" title="Detection">6. Detection</a>
  12 KB (1,790 words) - 16:14, 26 October 2012
• Team:Paris Bettencourt/Human Practice/WikiScreen
  ...rose (e.g in the soil, on human skin). The deactivation of expression will lead to a build up of mazF and the death of the cell". ...etic biology. Biosensors can be applied in a wide range of uses - from the detection of environmental toxics up to clinical diagnostics. Because cells have to s
  32 KB (4,752 words) - 02:59, 27 September 2012
• Team:Nanjing China Bio/notbook
  ...toxicity, expression of anti-cancer agents, and gene-triggering strategies detection of bacterial therapies. Each group has two students to work together on re
  11 KB (1,563 words) - 07:45, 25 September 2012
• Team:TU-Delft/part1
  ...ivity and selectivity of the of the olfactory system it can be of value in detection of environmental toxins [2] or pharmaceutical screening. In this iGEM proje ..., indicating that constitutive mRNA expression by the GPD promoter did not lead to constitutive protein expression in all cells.
  17 KB (2,804 words) - 13:05, 17 December 2012
• Team:Nanjing China Bio/notebook
  ...toxicity, expression of anti-cancer agents, and gene-triggering strategies detection of bacterial therapies. Each group has two students to work together on re
  11 KB (1,559 words) - 16:47, 26 September 2012
• Team:TU-Eindhoven/Lab test
  ...fferent GECO proteins at last. To make sure that the transformations would lead to the desired strain, it was decided to start the first step with transfor <h3>Detection of GECO protein in yeast</h3>
  8 KB (1,319 words) - 01:42, 27 September 2012
• Team:NTU-Taida/Human Practice/Safety-in-iGEM
  <p style="text-indent: 2em;">After the detection, the bacteria have to be destroyed in case the drug-resistant ability sprea ... abandoned due to its virulent characteristics in some bacteria, which may lead to pathogenic consequences (ex: using pili of ''Pseudomonas aeruginosa'', w
  21 KB (3,264 words) - 19:42, 26 October 2012