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From 2012.igem.org

Auxin toxicity test in tadpoles

The purpose of this experiement

A recent study led by Japanese researches implemented a rapid protein depletion system using Auxin, a plant hormone. The Auxin-Inducible Depletion (AID) system allowed a rapid and reversible degradation of target proteins in response to Auxin (indole-3-acetic acid (IAA) and 1-naphthaleneacetic acid (NAA)). The originality of this project lies in the fact that the AID system has been transplanted in non-plant cells as well as yeast and cell lines derived from chicken, mouse, hamster, monkey and human cells. Concerning cell treatment by the adding of Auxin in media, Kohei Nishimura et al. carried out experiences using 500µM of Auxin. Our project aimed to integrate the AID system in tadpoles for the control of protein synthesis in specific organ (link to exp). First we planned an Auxin toxicity test (IAA and NAA) in order to determine the suitable Auxin concentration for tadpoles. Experiments were conducted in [0, 125, 250, and 500] µM Auxin over 3 days and each morning the media were renewed.(Preparation of different media for embryos development see experiments link).

Results