Team:Uppsala University/Notebook

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Team Uppsala University – iGEM 2012


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Week 2

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Tuesday 26 June

Participant: Pikkei
Experiment:
Re-streak from agar stabs:
pSB1C3 - BBa_K322922 (cat + sacB)
pSB1C3 - BBa_K322921 (sacB)
BBa_K157000 - BBa_K243000 (Fok_a)
BBa_K157000 - BBa_K243001 (Fok_i)

Participant: Karl, Sabri, Pikkei
Experiment:
Take up from kit and transform:
pSB1A2 - Plac (BBa_R0010) from plate 1, well 1D
pSB2K3 - pBAD (BBa_I50500) from plate 1, well 14N
pSB1A2 - PlacQ (BBa_K091111) from plate 5, well 12C

Participant: Emy, Caroline
Experiment:
Start o/n culture:
pSB1C3 - tetA
pSB1C3 - CTX-M-15
pSB1C3 - ACC(6')_lb_cr
pSB1C3 - SYFP2
pSB1C3 - red
pSB3C5 - red
pSB3T5 - red
pSB1A3 - red
pSB4A5 - red
pSB1A2 - red
pSB1K3 - J23101
pSB1K3 - red
pSB3K3 - red
MG1655 pSB4K1 -lacIs (N246Q)

Wednesday 27 June

Participant: Pikkei
Experiment: Colony PCR and o/n culture
18. pSB1C3 – cat-sacB
19. pSB1C3 - sacB
20. BBa_K157000 – Fok_a
21. BBa_K157000 – Fok_i
Screened two clones of each
Left in PCR-machine o/n

Participant: Sabri, Fredrik
Experiment: Re-streak
15. pSB1A2 - Plac
17. pSB1A2 - PlacQ

Participant: Sibel, Lisa, Sabri, Olle, Hampus, Caroline, Emy, Joakim, Joel, Anna, Anders)
Experiment: Plasmid Prep and Glycerol Stock
4. pSB1A3 - red
5. pSB4A5 - red
12. pSB1A2 - B0034
13. pSB1K3 - J23101
14. MG1655 - pSB4K1 - lacI (N246Q)


Week 1

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Prepared TOP10 competent cells
Prepared LB medium, TEB medium and selective agarplates

Sponsors






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