Team:Uppsala University/Notebook
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Week 2Back to top |
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Tuesday 26 June
Participant: Pikkei Experiment: Re-streak from agar stabs: pSB1C3 - BBa_K322922 (cat + sacB) pSB1C3 - BBa_K322921 (sacB) BBa_K157000 - BBa_K243000 (Fok_a) BBa_K157000 - BBa_K243001 (Fok_i) Participant: Karl, Sabri, Pikkei Experiment: Take up from kit and transform: pSB1A2 - Plac (BBa_R0010) from plate 1, well 1D pSB2K3 - pBAD (BBa_I50500) from plate 1, well 14N pSB1A2 - PlacQ (BBa_K091111) from plate 5, well 12C Participant: Emy, Caroline Experiment: Start o/n culture: pSB1C3 - tetA pSB1C3 - CTX-M-15 pSB1C3 - ACC(6')_lb_cr pSB1C3 - SYFP2 pSB1C3 - red pSB3C5 - red pSB3T5 - red pSB1A3 - red pSB4A5 - red pSB1A2 - red pSB1K3 - J23101 pSB1K3 - red pSB3K3 - red MG1655 pSB4K1 -lacIs (N246Q) Wednesday 27 June Participant: Pikkei Experiment: Colony PCR and o/n culture 18. pSB1C3 – cat-sacB 19. pSB1C3 - sacB 20. BBa_K157000 – Fok_a 21. BBa_K157000 – Fok_i Screened two clones of each Left in PCR-machine o/n Participant: Sabri, Fredrik Experiment: Re-streak 15. pSB1A2 - Plac 17. pSB1A2 - PlacQ Participant: Sibel, Lisa, Sabri, Olle, Hampus, Caroline, Emy, Joakim, Joel, Anna, Anders) Experiment: Plasmid Prep and Glycerol Stock 4. pSB1A3 - red 5. pSB4A5 - red 12. pSB1A2 - B0034 13. pSB1K3 - J23101 14. MG1655 - pSB4K1 - lacI (N246Q) |
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Week 1Back to top |
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Prepared TOP10 competent cells
Prepared LB medium, TEB medium and selective agarplates |