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Team:Alberta/Project
From 2012.igem.org
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Overall project
A microbial color wheel
This year, the University of Alberta team is developing a biological sensor circuit with three pigment colours to generate a multiple coloured output. We developed this idea because traditional biological reporters have been limited to a small set of fluorescent proteins and colour genes, which produce only an all-or-none output. Furthermore, having only a single channel of output limits the application of other sensors in a single biological device. Therefore, it is clear that the traditional sensor system requires easy-to-use bioreporters that are capable of responding to chemical gradients and mixing independent output channels. To construct the biosensor circuit, we used existing genetic parts pioneered by the 2009 and 2010 iGEM teams, which will result in the circuit being capable of responding to chemical gradients and producing a multi-coloured output in the form of a colour wheel. This biosensor will become the new age of easy–to-read reporters that are incorporated into new versions of the Genomikon kit, which directly impact both research and education usage.
