Team:Michigan/Notebook/Protocols

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Protocols

General

Plate Production

Cloning

Removing BioBricks from the Registry WellsFile:MichRem.pdf

Production of Competent CellsFile:MichRem.pdf

Heat Shock Transformation

DNA Purification

Restriction Enzyme Digest

Ligation

PCR (Phusion)

Colony PCR

Characterization

Transmission Electron Microscopy Protocol

FimE Asymmetrical Digest Assay Protocol