Week 1

'''Friday'''

Today we started with a briefing from Mustafa where the main action points of the day were discussed. We had been planning the project of this year.We determined our parts that will be necessary:
*K124014 holin
*K559010 halorhodopsin+terminator(X2)
*K112806 endolysine/T4
*K112808 endolysine/holin/CMV/antiholin
These 4 parts was diluted and tagged. Then we coded these parts:
*C1:J23100 cons-promoter   
*C2:CMV promoter 
*P1:CL promoter 
*H1:Holorodopsin 
*P2:IPTG 
*L1:endolsin 
*L2:holin 
*l3:eth+antiholin

We prepared 35 plates and 200 ml LB broth for next experiments.
*Transformation (L1,L2,L3) 

*C1,C2 streak 
*Coloniese are incubated in liquid culture.(C1)

'''Saturday'''

* Coloniese are incubated liquid culture.( 1,5 ml AMP ) for :
C1(x2),C2,N1,L2,L3. 
* We made this process 2 times because of high mistake risk for first times

'''Sunday''' 

* Single colony isolation has made for C1-x,C1-y,C2,L1,L2 and L3.
* C1-x and C1-y are digested with EcoR1 and Pst1.
* We prepared liquid culture for C2,L1,L3 again.Because there was not enough DNA for digestion. 

'''Monday'''

* Single colony isolation has been made for L1,L2 and L3.
* Digestion has been made with EcoR1 and Pst1 for L1,L3 and C2.
* electrophoresis was performed for C1-x,C2,L1,L2 and L3.
* After electrophoresis,results of L1,L3 was true but the others was wrong.

'''Tuesday'''

* Digestion has been made with Xba1 and Pst1 for L1 and L3.
* 34 mg/ml chloramphenicol included 49 tubes was prepared. 
* Chloramphenicol included 17 plates was prepared.
* IPTG induceble promoter was coded as P2.
* Transformation has been made for H1,L1P2 and L3P2
* We prepared liquid culture for C1,C2,L2

'''Wednesday'''

* Single colony isolation has been made for C1,C2,L2
* We prepared liquid culture for C2 again.Because there was not enough DNA for digestion.
* C1 and L2 are digested.
* electrophoresis was performed for C1 and L2.
* The result of C1 was true but the result of L2 was wrong so a new digestion will been performed and after that electrophoresis will been performed for new L2 and old L2.
* We prepared liquid culture for P2L1,P2L3,C2,H1 and L2.

'''Thursday'''

* We made isolation for P2L1,P2L3,H1,C2,L2 and then digestion has been made with EcoR1 and Pst1 for all of these parts.After that electrophoresis was performed for these parts.
* The results of P2L1 and P2L3 might be true , we are not sure about this so we need some expert assistance and we need to sequence these part for being sure. Unfortunately the result of L2 was wrong again.
* We prepared a new liquid culture for L2.