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Team:Fatih-Medical/Sherlocoli/Modelling
From 2012.igem.org
Modelling
As Fatih Medical team we aim to make Cancer diagnosis by means of bacteria and Circulating Tumor Cells (CTC) interaction. CTCs have exceeded Epithelial Cell Adhesion Molecules (EpCam) on their cell membranes. On EpCam surface there are a lot of binding sites; identifying these binding sites we searched for anti-EpCams and found out that the most appropriate is C215 so we decide to operate with C215. In order to activate system that we used in Cancer Detection Module the Outer Membrane Protein A (OmpA) must be dimerized therefore we will use two binding sites on EpCam surface. (Figure 1)
Figure 1
When EpCam binds to the C215 that attached to the OmpA Sherlocoli will catch CTC thereby trigger activation of system and start module work. (Figure 2)
Figure 2
Because of our team consists of only medical students we didn’t have any experience in modeling but thanks to some team members who worked with MATLAB SİMBİOLOGY program and could make rational equations finally we have our modeling.
After analyzing EpCam anti-EpCam interactions from different articles[1] we’ve reached to the related data that characterise it. (table 1)
