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1
Synechocystis 6803 Media
1.1
1L agar for freshwater cyanobacteria (no glucose)
1.2
1L agar for freshwater cyanobacteria (with glucose)
1.3
1L liquid media for freshwater cyanobacteria
Synechocystis 6803 Media
1L agar for freshwater cyanobacteria (no glucose)
Mix 10 g agar and 1 mM thiosulfate (= 0.248 g), top off with H20 to 500 mL total volume.
Autoclave the product of (1)
Mix 20mL 50x BG-11 solution and 480 mL of H20
Autoclave the product of (3)
Mix (2) and (4), pour plates and let cool
1L agar for freshwater cyanobacteria (with glucose)
Mix 10 g agar and 1mM thiosulfate (= 0.248 g), top off with H20 to 500 mL total volume
Autoclave the product of (1)
Mix 20 mL 50x BG-11 solution and 230 mL of H20
Autoclave the product of (3)
Mix 5 mL glucose and 245 mL of H20. [2]
Autoclave the product of (5)
Mix (2) and (4) and (6), pour plates and let cool
1L liquid media for freshwater cyanobacteria
Add 20 mL 50x BG-11 and 1 mM thiosulfate (= 0.248 g), top off with H20 to 1L total volume
Filter the solution into the desired container with a 0.2 µm filter.