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User contributions
From 2012.igem.org
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- 15:17, 24 July 2012 (diff | hist) Team:Cambridge/Safety (→Additional safety information)
- 20:02, 23 July 2012 (diff | hist) N Team:Cambridge/HumanPractices (Created page with "==Human Practices== '''N.B.''' This page is a work in progress and will be added to and improved over the course of our project. 23.07.2012 Started our human practices in ear...") (top)
- 13:56, 23 July 2012 (diff | hist) Team:Cambridge/Safety (→Additional safety information)
- 13:56, 23 July 2012 (diff | hist) Team:Cambridge/Safety (→Additional safety information)
- 13:55, 23 July 2012 (diff | hist) Team:Cambridge/Safety (→iGEM safety questions)
- 09:21, 23 July 2012 (diff | hist) Team:Cambridge/Safety/MSDS (→Materials Safety Data Sheets)
- 08:35, 23 July 2012 (diff | hist) Team:Cambridge/Safety/MSDS (→Materials Safety Data Sheets)
- 08:34, 23 July 2012 (diff | hist) N Team:Cambridge/Safety/MSDS (Created page with "==Materials Safety Data Sheets== {| style="color:#1b2c8a;background-color:#3ae2e8;" cellpadding="3" cellspacing="0" border="1" bordercolor="#fff" width="62%" align="center" !ali...")
- 15:15, 22 July 2012 (diff | hist) Team:Cambridge/Safety
- 12:47, 19 July 2012 (diff | hist) Team:Cambridge/Team (→Jolyon)
- 12:44, 19 July 2012 (diff | hist) Team:Cambridge/Team
- 16:30, 18 July 2012 (diff | hist) Team:Cambridge/Team (→Charlie)
- 16:29, 18 July 2012 (diff | hist) N File:Cambridge Charlie.png
- 15:46, 16 July 2012 (diff | hist) N Team:Cambridge/Protocols/TransformationofE.coli (Created page with "==Transformation of E.coli== A simple method of transforming competent E.coli cells with your DNA of choice. ===Theory=== Artificial transformation is a process whereby E.coli ...")
- 15:45, 16 July 2012 (diff | hist) Team:Cambridge/Protocols
- 15:40, 16 July 2012 (diff | hist) Team:Cambridge/Protocols/TransformationofB.subtilis
- 11:26, 13 July 2012 (diff | hist) N Team:Cambridge/SDSPAGE (Created page with "==Protein Analysis by SDS PAGE== These are some notes made in preparation for running SDS PAGE to verify we have Reflectin. Like DNA gels PAGE gels can be made with various diff...")
- 11:12, 13 July 2012 (diff | hist) N Team:Cambridge/RestrictionDigest (Created page with "==Restriction Enzyme Digestion== A method that allows to create a restriction map of a given DNA fragment. Widely used to test for the correct integration of a cloned sequence in...")
- 10:58, 13 July 2012 (diff | hist) N Team:Cambridge/MiniPrep (Created page with "==MiniPrep== A method used to extract DNA from bacterial cells. ===Theory=== The miniprep kit relies on a column, which fits into a microcentrifuge tube which has alterable aff...")
- 10:42, 13 July 2012 (diff | hist) N Team:Cambridge/TransformationofB.subtilis (Created page with "==Transformation of ''Bacillus subtilis''== A technique used to introduce foreign DNA into competent Bacillus cells. ===Theory=== ''Bacillus subtilis'' is a rod-shaped, soil-dwe...")
- 10:36, 13 July 2012 (diff | hist) N Team:Cambridge/GelExtractionofDNA (Created page with "==Gel Extraction of DNA (Spin Column Extraction)== A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis Gel ext...")
- 10:30, 13 July 2012 (diff | hist) Team:Cambridge/GelElectrophoresis (→Gel Electrophoresis)
- 10:25, 13 July 2012 (diff | hist) N Team:Cambridge/GelElectrophoresis (Created page with "==Gel Electrophoresis== A method to separate DNA strands of different lengths. ===Theory=== DNA is negatively charged due to the phosphate ions (PO<sub>4</sub><sup>3-</sup>) ba...")
- 10:07, 13 July 2012 (diff | hist) Team:Cambridge/Protocols
- 15:39, 12 July 2012 (diff | hist) Team:Cambridge/Gibsonassembly (→Gibson Assembly:)
- 15:38, 12 July 2012 (diff | hist) Team:Cambridge/Gibsonassembly (→Gibson Assembly:)
- 15:37, 12 July 2012 (diff | hist) Team:Cambridge/PCRcolony (→Colony PCR:)
- 15:37, 12 July 2012 (diff | hist) Team:Cambridge/PCRcolony (→Colony PCR:)
- 15:30, 12 July 2012 (diff | hist) Team:Cambridge/PCRprotocol (→PCR using Phusion DNA polymerase:)
- 15:29, 12 July 2012 (diff | hist) Team:Cambridge/PCRprotocol (→PCR using Phusion DNA polymerase:)
- 15:13, 12 July 2012 (diff | hist) Team:Cambridge/Gibsonassembly (→Gibson Assembly:)
- 15:08, 12 July 2012 (diff | hist) Team:Cambridge/Gibsonassembly (→Gibson Assembly:)
- 15:08, 12 July 2012 (diff | hist) Team:Cambridge/Gibsonassembly (→Gibson Assembly:)
- 14:59, 12 July 2012 (diff | hist) N Team:Cambridge/Gibsonassembly (Created page with "==Gibson Assembly:== There are no cells involved in this procedure so biohazard risks are very small. Coats should still be worn however and gloves are particularly important t...")
- 13:17, 12 July 2012 (diff | hist) Team:Cambridge/Protocols
- 13:15, 12 July 2012 (diff | hist) Team:Cambridge/PCRcolony (→Colony PCR:)
- 13:13, 12 July 2012 (diff | hist) Team:Cambridge/PCRprotocol (→PCR using Phusion DNA polymerase:)
- 13:11, 12 July 2012 (diff | hist) Team:Cambridge/PCRprotocol (→PCR using Phusion DNA polymerase:)
- 13:08, 12 July 2012 (diff | hist) Team:Cambridge/Protocols
- 13:07, 12 July 2012 (diff | hist) Team:Cambridge/Protocols
- 13:05, 12 July 2012 (diff | hist) Team:Cambridge/Protocols
- 13:04, 12 July 2012 (diff | hist) Team:Cambridge/Protocols
- 13:04, 12 July 2012 (diff | hist) Team:Cambridge/Protocols
- 13:03, 12 July 2012 (diff | hist) Team:Cambridge/Protocols
- 13:01, 12 July 2012 (diff | hist) N Team:Cambridge/PCRcolony (Created page with "==Colony PCR:== Used to amplify DNA directly from cell culture, the reaction is the same as for normal PCR but with modified reaction composition and cycle settings, here shown...")
- 11:43, 12 July 2012 (diff | hist) Team:Cambridge/Protocols
- 11:42, 12 July 2012 (diff | hist) N Team:Cambridge/PCRprotocol (Created page with "==PCR using Phusion DNA polymerase:== If this reaction is to be done from scratch, add the reagents in the order they are listed in the table. When multiple PCR experiments nee...")
- 10:48, 12 July 2012 (diff | hist) N Team:Cambridge/Protocols (Created page with "* PCR using Phusion DNA polymerase * Colony PCR * Gibson Assembly")
- 10:45, 12 July 2012 (diff | hist) Team:Cambridge/Notebook (→Diary)
- 14:40, 2 July 2012 (diff | hist) Team:Cambridge
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