Team:WashU/Saffroninakansynecho

From 2012.igem.org

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Our project consisted of three different phases. <br>
Our project consisted of three different phases. <br>
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Phase 1: Produce zeaxanthin in our bacteria. <br>
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Phase 1: Produce zeaxanthin in our two chosen organisms (<i>E. coli</i> and <i>Synechocystis</i>). <br>
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Phase 2: Insert our genes into the bacteria and produce the proteins needed to convert zeaxanthin into crocin (ZCD and UGTCS2 proteins). <br>
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Phase 2: Overproduce the two genes needed for the conversion of crocin and saffranal (ZCD and UGTCs2 proteins). <br>
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Phase 3: Express crocin and characterize its expression. <br>
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Phase 3: Synthesize crocin <i> in vivo </i> and characterize its production <br>
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Revision as of 19:01, 17 August 2012




Overview

Our project consisted of three different phases.
Phase 1: Produce zeaxanthin in our two chosen organisms (E. coli and Synechocystis).
Phase 2: Overproduce the two genes needed for the conversion of crocin and saffranal (ZCD and UGTCs2 proteins).
Phase 3: Synthesize crocin in vivo and characterize its production

Saffron in a Kan

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