Team:Osaka/week1
From 2012.igem.org
(Difference between revisions)
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!number!!Part Name!!Resistance!!Description | !number!!Part Name!!Resistance!!Description | ||
|- | |- | ||
- | |124||<bbpart>BBa_K602015</bbpart>||C|| < | + | |124||<bbpart>BBa_K602015</bbpart>||C|| <i>D. radiodurans<i> PprI+PprA expression device |
|- | |- | ||
- | |125||<bbpart>BBa_K602016</bbpart>||C|| < | + | |125||<bbpart>BBa_K602016</bbpart>||C|| <i>D. radiodurans<i> PprI+RecA expression device |
|- | |- | ||
- | |126||<bbpart>BBa_K602017</bbpart>||C|| < | + | |126||<bbpart>BBa_K602017</bbpart>||C|| <i>D. radiodurans<i> PprA+RecA expression device |
|- | |- | ||
- | |127||<bbpart>BBa_K602018</bbpart>||C|| < | + | |127||<bbpart>BBa_K602018</bbpart>||C|| <i>D. radiodurans<i> PprI+PprM expression device |
|- | |- | ||
- | |128||<bbpart>BBa_K602019</bbpart>||C|| < | + | |128||<bbpart>BBa_K602019</bbpart>||C|| <i>D. radiodurans<i> PprA+PprM expression device |
|- | |- | ||
- | |129||<bbpart>BBa_K602020</bbpart>||C|| < | + | |129||<bbpart>BBa_K602020</bbpart>||C|| <i>D. radiodurans<i> PprM+RecA expression device |
|} | |} |
Revision as of 04:51, 17 August 2012
August 1 (Wed)
- Preparation of LB agar plates (Amp)
- Restriction digests of parts (See Table 1)
number | Part Name | Resistance | Description |
---|---|---|---|
124 | <bbpart>BBa_K602015</bbpart> | C | D. radiodurans<i> PprI+PprA expression device |
125 | <bbpart>BBa_K602016</bbpart> | C | <i>D. radiodurans<i> PprI+RecA expression device |
126 | <bbpart>BBa_K602017</bbpart> | C | <i>D. radiodurans<i> PprA+RecA expression device |
127 | <bbpart>BBa_K602018</bbpart> | C | <i>D. radiodurans<i> PprI+PprM expression device |
128 | <bbpart>BBa_K602019</bbpart> | C | <i>D. radiodurans<i> PprA+PprM expression device |
129 | <bbpart>BBa_K602020</bbpart> | C | <i>D. radiodurans<i> PprM+RecA expression device |
- Ligation
- 143(A): 124+1-1G(vector)
- 144(A): 125+1-1G(vector)
- 145(A): 126+1-1G(vector)
- 146(A): 127+1-1G(vector)
- 147(A): 128+1-1G(vector)
- 148(A): 129+1-1G(vector)
- 149(A): 138+1-1G(vector)
- 150(A): 139+1-1G(vector)
- 151(A): 140+1-1G(vector)
- 152(A): 141+1-1G(vector)
- Transformation of ligation products