Team:Uppsala University/Notebook

From 2012.igem.org

(Difference between revisions)
Line 42: Line 42:
<br>
<br>
<br> Participant: Pikkei  
<br> Participant: Pikkei  
-
<br> Experiment:  
+
<br> Experiment: Re-streak from agar stabs:  
-
<br> Re-streak from agar stabs:  
+
<br>  pSB1C3 - BBa_K322922 (cat + sacB)
<br>  pSB1C3 - BBa_K322922 (cat + sacB)
<br>  pSB1C3 - BBa_K322921 (sacB)
<br>  pSB1C3 - BBa_K322921 (sacB)
Line 50: Line 49:
<br>
<br>
<br>Participant: Karl, Sabri, Pikkei
<br>Participant: Karl, Sabri, Pikkei
-
<br>Experiment:
+
<br>Experiment: Take up from kit and transform:
-
<br>Take up from kit and transform:
+
<br>  pSB1A2 - Plac (BBa_R0010) from plate 1, well 1D
<br>  pSB1A2 - Plac (BBa_R0010) from plate 1, well 1D
<br>  pSB2K3 - pBAD (BBa_I50500) from plate 1, well 14N
<br>  pSB2K3 - pBAD (BBa_I50500) from plate 1, well 14N
Line 57: Line 55:
<br>
<br>
<br>Participant: Emy, Caroline
<br>Participant: Emy, Caroline
-
<br>Experiment:
+
<br>Experiment: Start o/n culture:
-
<br>Start o/n culture:
+
<br>  pSB1C3 - tetA
<br>  pSB1C3 - tetA
<br>  pSB1C3 - CTX-M-15
<br>  pSB1C3 - CTX-M-15
Line 73: Line 70:
<br>  pSB3K3 - red
<br>  pSB3K3 - red
<br>  MG1655 pSB4K1 -lacIs (N246Q)
<br>  MG1655 pSB4K1 -lacIs (N246Q)
 +
<br>
<br>
<br>
<br>Wednesday 27 June
<br>Wednesday 27 June
Line 97: Line 95:
<br>  13. pSB1K3 - J23101
<br>  13. pSB1K3 - J23101
<br>  14. MG1655 - pSB4K1 - lacI (N246Q)
<br>  14. MG1655 - pSB4K1 - lacI (N246Q)
 +
<br>  pSB1C3 - CTX-M-15
 +
<br>  pSB1C3 - ACC(6')_lb_cr
 +
<br>  pSB1C3 - SYFP2
 +
<br>  pSB1K3 - red
 +
<br>  pSB3K3 - red
 +
<br>  pSB1C3 - tetA
 +
<br>  1. pSB1C3 - red
 +
<br>  2. pSB3C5 - red
 +
<br>  3. pSB3T5 - red
 +
<br>Followed Plasmid Purification Protocol
 +
<br>
 +
<br>Participant: Hampus, Sabri
 +
<br>Experiment: Cloning of 22. pEL3S15 - red and 13. pSB1K3 - J23101
 +
<br>  30. pEL3S15 - J23101
 +
<br>Cut with X and P
 +
<br>
 +
<br>Participant: Caroline, Emy, Ellen, Joel
 +
<br>Experiment: Assembly
 +
<br>  pSB3T5 - lacIs - TλT1
 +
<br>  pSB3T5 - SYFP2 - TλT1
 +
<br>  pSB3T5 - T5lac - B0034
 +
<br>Followed BioBrick Assembly Manual
 +
<br>
 +
<br>
 +
<br>Thursday 28 June
 +
<br>
 +
<br>Participant: Joel
 +
<br>Experiment: Gel Electrophoresis of yesterday's screening products
 +
<br>  18.1-2 pSB1C3 – cat-sacB
 +
<br>  19.1-2 pSB1C3 - sacB
 +
<br>  20.1-2 BBa_K157000 – Fok_a
 +
<br>  21.1-2 BBa_K157000 – Fok_i
 +
<br>Result: None of the band were correct
 +
<br>
 +
<br>Participant: Caroline, Emy, Ellen, Joel
 +
<br>Experiment: Transformation
 +
<br>  pSB3T5 - lacIs - TλT1
 +
<br>  pSB3T5 - SYFP2 - TλT1
 +
<br>  pSB3T5 - T5lac - B0034
 +
<br>Followed TOP10 transformation protocol
 +
<br>
 +
<br>Participant: Fredrik, Julia, Joakim K, Olle
 +
<br>Experiment: Plasmid Prep and Glycerol Stock
 +
<br>  18.1-2 pSB1C3 – cat-sacB
 +
<br>  19.1-2 pSB1C3 - sacB
 +
<br>  20.1-2 BBa_K157000 – Fok_a
 +
<br>  21.1-2 BBa_K157000 – Fok_i
 +
<br>Followed Plasmid Purification Protocol
 +
<br>
 +
<br>Participant: Lisa, Sabri
 +
<br>Experiment: o/n culture
 +
<br>  .1-2 pSB1A2 - Plac
 +
<br>  .1-2 pSB1A2 - PlacQ
 +
<br>
 +
<br>Participant: Joakim, Anna
 +
<br>Experiment: Make new SOB & SOC
 +
<br>
 +
<br>Participant: Tobias, Christoffer
 +
<br>Experiment: Transformation
 +
<br>  pEL3S15 - J23101
 +
<br>
 +
<br>
 +
<br>Friday 29 June
 +
<br>
 +
<br>Participant: Julia, Hampus, Tobias
 +
<br>Experiment: Re-streak
 +
<br>  pEL3S15 - J23101
 +
<br>
 +
<br>Participant: Olle, Sabri, Lisa
 +
<br>Experiment: Transformation
 +
<br>  23. pSB1A1 - P1004 (cat)  from registry well 7B
 +
<br>  24. pSB2K3 - tetR  from registry well 16P
 +
<br>
 +
<br>Participant: Sabri, Lisa, Josefin, Niklas
 +
<br>Experiment: Assembly
 +
<br>  pSB1K3 - B0034 - SYFP2
 +
<br>  pSB3C5 - J23101 - tet inv
 +
<br>
 +
<br>Participant: Caroline, Emy, Ellen, Joel, Tobias
 +
<br>Experiment: Assembly
 +
<br>  pSB3T5 - PlacQ - ACC(6')
 +
<br>  pSB3T5 - PlacQ - CTX-M-15
 +
<br>  pSB3T5 - PlacQ - tetA
 +
<br>  pSB3K3 - Plac - B0034 - SYFP2
 +
<br>  pSB1C3 - TλT1
 +
<br>
 +
<br>Participant: Caroline, Emy, Ellen, Joel, Tobias
 +
<br>Experiment: Re-streak
 +
<br>  pSB3T5 - lacIs - TλT1
 +
<br>  pSB3T5 - SYFP2 - TλT1
 +
<br>  pSB3T5 - T5lac - B0034
 +
<br>
 +
<br>Participant: Anna, Ellen
 +
<br>Experiment: o/n culture
 +
<br>  J23116
 +
<br>
 +
<br>Participant: Anna, Ellen
 +
<br>Experiment: Plasmid Prep
 +
<br>  15.1-2 pSB1A2 - Plac
 +
<br>  17.1-2 pSB1A2 - PlacQ
 +
<br>
 +
<br>
 +
<br>Saturday 30 June
 +
<br>
 +
<br>Participant: Hampus, Julia, Tobias
 +
<br>Experiment: Screening
 +
<br>  pEL3S15 - J23101
 +
<br>  pSB3T5 - lacIs -
 +
<br>  pSB3T5 - SYFP2 -
 +
<br>  pSB3T5 - pT5lac - B0034
 +
<br>Result: Bad gel
 +
<br>
 +
<br>
 +
<br>Participant: Caroline, Fredrik, Emy, Hampus
 +
<br>Experiment: o/n culture
 +
<br>  30.1-4 pEL3S15 - J23101
 +
<br>  31.1-4 pEL3T5 - lacIs -
 +
<br>  32.1-4 pSB3T5 - SYFP2 -
 +
<br>  33.1-2 pSB3T5- pT5lac - B0034
 +
<br>  20.1 BBa_K157000 - Fok_a
 +
<br>  21.1 BBa_K157000 - Fok_i
 +
<br>
 +
<br>Participant: Ellen, Emy, Caroline, Joel
 +
<br>Experiment: Plasmid Prep
 +
<br>  J61002 - J23106
 +
<br>  J61002 - J23116
 +
<br>
 +
<br>Participant: Ellen, Emy, Caroline, Joel
 +
<br>Experiment: Transformera
 +
<br>  pSB3T5 - PlacIq - AAC(6')
 +
<br>  pSB3T5 - PlacIq - CTX-M
 +
<br>  pSB3T5 - PlacIq - tetA
 +
<br>  pSB3K3 - Plac - B0034 - SYFP2
 +
<br>  pSB1C3 - T....
 +
<br>
 +
<br>Participant: Ellen, Emy, Caroline, Joel
 +
<br>Experiment: Assembly
 +
<br>  pSB3T5 - J23106 - AAC(6')
 +
<br>  pSB3T5 - J23106 - CTX-M
 +
<br>  pSB3T5 - J23106 - tetA
 +
<br>  pSB3T5 - J23116 - AAC(6')
 +
<br>  pSB3T5 - J23116 - CTX-M
 +
<br>  pSB3T5 - J23116 - tetA
 +
<br>
 +
<br>Participant: Fredrik
 +
<br>Experiment: Transformation
 +
<br>  24. pSB2K3 - tet inv
 +
<br>  39. pSB1K3 - B0034 - SYFP2
 +
<br>  40. pSB3C5 - J23101 - tet inv
 +
<br>
 +
<br>Participant: Fredrik
 +
<br>Experiment: Re-streak
 +
<br>  16. pSB2K3 - pBAD
 +
<br>  23. pSB1A1 - P1004
 +
<br>  24. pSB1K3 - tet inv
 +
<br>
 +
<br>
 +
<br>Sunday 1 July
 +
<br>
 +
<br>Participant: Hampus, Julia, Lisa
 +
<br>Experiment: Ran new gel of yesterday's screening
 +
<br>  pEL3S15 - J23101
 +
<br>  pSB3T5 - lacIs -
 +
<br>  pSB3T5 - SYFP2 -
 +
<br>  pSB3T5 - pT5lac - B0034
 +
<br>Result: Contamination
 +
<br>
 +
<br>Participant:
 +
<br>Experiment:
 +
<br> 
 +
<br> 
 +
<br>
 +
<br>
 +
<br>Participant:
 +
<br>Experiment:
 +
<br> 
 +
<br> 
 +
<br>
 +
<br>
 +
<br>Participant:
 +
<br>Experiment:
 +
<br> 
 +
<br> 
 +
<br>
 +
<br>
 +
<br>Participant:
 +
<br>Experiment:
 +
<br> 
 +
<br> 
 +
<br>
</td>
</td>

Revision as of 15:44, 28 July 2012

Team Uppsala University – iGEM 2012


This page is currently under construction.



Week 2

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Tuesday 26 June

Participant: Pikkei
Experiment: Re-streak from agar stabs:
pSB1C3 - BBa_K322922 (cat + sacB)
pSB1C3 - BBa_K322921 (sacB)
BBa_K157000 - BBa_K243000 (Fok_a)
BBa_K157000 - BBa_K243001 (Fok_i)

Participant: Karl, Sabri, Pikkei
Experiment: Take up from kit and transform:
pSB1A2 - Plac (BBa_R0010) from plate 1, well 1D
pSB2K3 - pBAD (BBa_I50500) from plate 1, well 14N
pSB1A2 - PlacQ (BBa_K091111) from plate 5, well 12C

Participant: Emy, Caroline
Experiment: Start o/n culture:
pSB1C3 - tetA
pSB1C3 - CTX-M-15
pSB1C3 - ACC(6')_lb_cr
pSB1C3 - SYFP2
pSB1C3 - red
pSB3C5 - red
pSB3T5 - red
pSB1A3 - red
pSB4A5 - red
pSB1A2 - red
pSB1K3 - J23101
pSB1K3 - red
pSB3K3 - red
MG1655 pSB4K1 -lacIs (N246Q)


Wednesday 27 June

Participant: Pikkei
Experiment: Colony PCR and o/n culture
18. pSB1C3 – cat-sacB
19. pSB1C3 - sacB
20. BBa_K157000 – Fok_a
21. BBa_K157000 – Fok_i
Screened two clones of each
Left in PCR-machine o/n

Participant: Sabri, Fredrik
Experiment: Re-streak
15. pSB1A2 - Plac
17. pSB1A2 - PlacQ

Participant: Sibel, Lisa, Sabri, Olle, Hampus, Caroline, Emy, Joakim, Joel, Anna, Anders)
Experiment: Plasmid Prep and Glycerol Stock
4. pSB1A3 - red
5. pSB4A5 - red
12. pSB1A2 - B0034
13. pSB1K3 - J23101
14. MG1655 - pSB4K1 - lacI (N246Q)
pSB1C3 - CTX-M-15
pSB1C3 - ACC(6')_lb_cr
pSB1C3 - SYFP2
pSB1K3 - red
pSB3K3 - red
pSB1C3 - tetA
1. pSB1C3 - red
2. pSB3C5 - red
3. pSB3T5 - red
Followed Plasmid Purification Protocol

Participant: Hampus, Sabri
Experiment: Cloning of 22. pEL3S15 - red and 13. pSB1K3 - J23101
30. pEL3S15 - J23101
Cut with X and P

Participant: Caroline, Emy, Ellen, Joel
Experiment: Assembly
pSB3T5 - lacIs - TλT1
pSB3T5 - SYFP2 - TλT1
pSB3T5 - T5lac - B0034
Followed BioBrick Assembly Manual


Thursday 28 June

Participant: Joel
Experiment: Gel Electrophoresis of yesterday's screening products
18.1-2 pSB1C3 – cat-sacB
19.1-2 pSB1C3 - sacB
20.1-2 BBa_K157000 – Fok_a
21.1-2 BBa_K157000 – Fok_i
Result: None of the band were correct

Participant: Caroline, Emy, Ellen, Joel
Experiment: Transformation
pSB3T5 - lacIs - TλT1
pSB3T5 - SYFP2 - TλT1
pSB3T5 - T5lac - B0034
Followed TOP10 transformation protocol

Participant: Fredrik, Julia, Joakim K, Olle
Experiment: Plasmid Prep and Glycerol Stock
18.1-2 pSB1C3 – cat-sacB
19.1-2 pSB1C3 - sacB
20.1-2 BBa_K157000 – Fok_a
21.1-2 BBa_K157000 – Fok_i
Followed Plasmid Purification Protocol

Participant: Lisa, Sabri
Experiment: o/n culture
.1-2 pSB1A2 - Plac
.1-2 pSB1A2 - PlacQ

Participant: Joakim, Anna
Experiment: Make new SOB & SOC

Participant: Tobias, Christoffer
Experiment: Transformation
pEL3S15 - J23101


Friday 29 June

Participant: Julia, Hampus, Tobias
Experiment: Re-streak
pEL3S15 - J23101

Participant: Olle, Sabri, Lisa
Experiment: Transformation
23. pSB1A1 - P1004 (cat) from registry well 7B
24. pSB2K3 - tetR from registry well 16P

Participant: Sabri, Lisa, Josefin, Niklas
Experiment: Assembly
pSB1K3 - B0034 - SYFP2
pSB3C5 - J23101 - tet inv

Participant: Caroline, Emy, Ellen, Joel, Tobias
Experiment: Assembly
pSB3T5 - PlacQ - ACC(6')
pSB3T5 - PlacQ - CTX-M-15
pSB3T5 - PlacQ - tetA
pSB3K3 - Plac - B0034 - SYFP2
pSB1C3 - TλT1

Participant: Caroline, Emy, Ellen, Joel, Tobias
Experiment: Re-streak
pSB3T5 - lacIs - TλT1
pSB3T5 - SYFP2 - TλT1
pSB3T5 - T5lac - B0034

Participant: Anna, Ellen
Experiment: o/n culture
J23116

Participant: Anna, Ellen
Experiment: Plasmid Prep
15.1-2 pSB1A2 - Plac
17.1-2 pSB1A2 - PlacQ


Saturday 30 June

Participant: Hampus, Julia, Tobias
Experiment: Screening
pEL3S15 - J23101
pSB3T5 - lacIs -
pSB3T5 - SYFP2 -
pSB3T5 - pT5lac - B0034
Result: Bad gel


Participant: Caroline, Fredrik, Emy, Hampus
Experiment: o/n culture
30.1-4 pEL3S15 - J23101
31.1-4 pEL3T5 - lacIs -
32.1-4 pSB3T5 - SYFP2 -
33.1-2 pSB3T5- pT5lac - B0034
20.1 BBa_K157000 - Fok_a
21.1 BBa_K157000 - Fok_i

Participant: Ellen, Emy, Caroline, Joel
Experiment: Plasmid Prep
J61002 - J23106
J61002 - J23116

Participant: Ellen, Emy, Caroline, Joel
Experiment: Transformera
pSB3T5 - PlacIq - AAC(6')
pSB3T5 - PlacIq - CTX-M
pSB3T5 - PlacIq - tetA
pSB3K3 - Plac - B0034 - SYFP2
pSB1C3 - T....

Participant: Ellen, Emy, Caroline, Joel
Experiment: Assembly
pSB3T5 - J23106 - AAC(6')
pSB3T5 - J23106 - CTX-M
pSB3T5 - J23106 - tetA
pSB3T5 - J23116 - AAC(6')
pSB3T5 - J23116 - CTX-M
pSB3T5 - J23116 - tetA

Participant: Fredrik
Experiment: Transformation
24. pSB2K3 - tet inv
39. pSB1K3 - B0034 - SYFP2
40. pSB3C5 - J23101 - tet inv

Participant: Fredrik
Experiment: Re-streak
16. pSB2K3 - pBAD
23. pSB1A1 - P1004
24. pSB1K3 - tet inv


Sunday 1 July

Participant: Hampus, Julia, Lisa
Experiment: Ran new gel of yesterday's screening
pEL3S15 - J23101
pSB3T5 - lacIs -
pSB3T5 - SYFP2 -
pSB3T5 - pT5lac - B0034
Result: Contamination

Participant:
Experiment:




Participant:
Experiment:




Participant:
Experiment:




Participant:
Experiment:




Week 1

Back to top
Prepared TOP10 competent cells
Prepared LB medium, TEB medium and selective agarplates

Sponsors






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