Team:EPF-Lausanne/Protocol/Gel

(Difference between revisions)

Revision as of 14:31, 1 July 2012

Agarose concentration depends on the size of the DNA to be run. Example using 1% agarose gel and small gel box (80 ml of gel):

Add 0.8 g of agarose to a 80 ml clean glass bottle.
Pour 1.6 ml of 50xTAE in a graduated cylinder. Fill up to 80 ml with di water.
Add the resulting 80 ml of 1xTAE to the glass bottle with agarose.
Microwave, at 7, the bottle (no cap!) until it boils.
Carefully remove bottle (can be super heated!) and check for the total absence of particles. Microwave again if needed.
Prepare a small gel box and fill it up with the agarose solution (maybe not the whole solutio is needed).
Add 3 µl (0.05 µl per ml of gel in the box) of Red Gel (it's in the iGEM drawer) and stirr until disolved.
UNFINISHED

@@ Line 11: / Line 11: @@
 Example using 1% agarose gel and small gel box (80 ml of gel):
-* Add 0.8 g of agarose to a 80 ml clean  glass bottle.
+# Add 0.8 g of agarose to a 80 ml clean  glass bottle.
-* Pour 1.6 ml of 50xTAE in a graduated cylinder. Fill up to 80 ml with di water.
+# Pour 1.6 ml of 50xTAE in a graduated cylinder. Fill up to 80 ml with di water.
-* Add the resulting 80 ml of 1xTAE to the glass bottle with agarose.
+# Add the resulting 80 ml of 1xTAE to the glass bottle with agarose.
-* Microwave, at 7, the bottle (no cap!) until it boils.
+# Microwave, at 7, the bottle (no cap!) until it boils.
-* Carefully remove bottle (can be super heated!) and check for the total absence of particles. Microwave again if needed.
+# Carefully remove bottle (can be super heated!) and check for the total absence of particles. Microwave again if needed.
-* Prepare a small gel box and fill it up with the agarose solution (maybe not the whole solutio is needed).
+# Prepare a small gel box and fill it up with the agarose solution (maybe not the whole solutio is needed).
-* Add 3 µl (0.05 µl per ml of gel in the box) of Red Gel (it's in the iGEM drawer) and stirr until disolved.
+# Add 3 µl (0.05 µl per ml of gel in the box) of Red Gel (it's in the iGEM drawer) and stirr until disolved.
-* UNFINISHED
+# UNFINISHED
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