Team:Shenzhen/Result/YAO.Suicider
From 2012.igem.org
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<p> 5. Centrifuging for 14,000rpm for 5sec, abandon the supermate, 0.1ml 1x TE suspense the cell precipitation, spread it to SD/Ura solid plat, 30°C culture reversely for 3 days.</p> | <p> 5. Centrifuging for 14,000rpm for 5sec, abandon the supermate, 0.1ml 1x TE suspense the cell precipitation, spread it to SD/Ura solid plat, 30°C culture reversely for 3 days.</p> | ||
<p> 6. Holin function identification: </p> | <p> 6. Holin function identification: </p> | ||
- | <p> Pick a better-cultured colony and inoculate it to 50ml YPDA medium, 30°C,220rpm culture reelingly to OD-0.1, transfer 20ml to 50ml sterile medium (alpha-galactose), versus is added by equivalent water. Measuring the OD value one time for one hour and drawing the curve. </p> | + | <p> Pick a better-cultured colony and inoculate it to 50ml YPDA medium, 30°C,220rpm culture reelingly to OD-0.1, transfer 20ml to 50ml sterile medium (alpha-galactose), versus is added by equivalent water. Measuring the OD value one time for one hour and drawing the curve. </p> |
<p> Result analyzation: </p> | <p> Result analyzation: </p> | ||
- | <p> | + | <p> If the bacteria in alpha-galactose grow slower that in water, that shows holing can work. </p></ul> |
</div> | </div> | ||
</div> | </div> | ||
{{:Team:Shenzhen/Temp/gallery.htm}} | {{:Team:Shenzhen/Temp/gallery.htm}} |
Revision as of 15:36, 25 September 2012