Team:Shenzhen/Project/YAO.Channel
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<p>Figure 2. Signal peptides directing proteins to mitochondria. 1) Three kinds of signal peptides. 2) Directing proteins toward TOM and TIM complex. 3) Protein transported to different destinations. </p></div> | <p>Figure 2. Signal peptides directing proteins to mitochondria. 1) Three kinds of signal peptides. 2) Directing proteins toward TOM and TIM complex. 3) Protein transported to different destinations. </p></div> | ||
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<ul><p> 2. Toc34 is a GTPase protein that functions as signal peptides receptor, N-terminally anchored at chloroplast outer membrane, just as Tom20 and Tom70 do in mitochondria. When equipped with Tom20 or Tom70 targeting peptide, it will theoretically be assembled onto mitochondrial outer membrane in the same way as Toc75, but with the assistance of Mim1 instead of SAM complex.</p></ul> | <ul><p> 2. Toc34 is a GTPase protein that functions as signal peptides receptor, N-terminally anchored at chloroplast outer membrane, just as Tom20 and Tom70 do in mitochondria. When equipped with Tom20 or Tom70 targeting peptide, it will theoretically be assembled onto mitochondrial outer membrane in the same way as Toc75, but with the assistance of Mim1 instead of SAM complex.</p></ul> | ||
<ul><p> 3. Presently we only focus on the approaches for assembly of TOC proteins as it’s much easier than the assembly of TIC proteins. Hopefully we would come up with a feasible way in future time to assemble TIC complex. </p></ul> | <ul><p> 3. Presently we only focus on the approaches for assembly of TOC proteins as it’s much easier than the assembly of TIC proteins. Hopefully we would come up with a feasible way in future time to assemble TIC complex. </p></ul> | ||
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<p>Figure 3. Assembling Toc75 & Tic110 onto mitochondria membranes. 1) Adding mitochondrial signal peptides to TOC & TIC proteins. 2) Directing of TOM & TIC proteins toward mitochondria. 3) TOC & TIC installed onto the membranes of mitochondria. </p></div> | <p>Figure 3. Assembling Toc75 & Tic110 onto mitochondria membranes. 1) Adding mitochondrial signal peptides to TOC & TIC proteins. 2) Directing of TOM & TIC proteins toward mitochondria. 3) TOC & TIC installed onto the membranes of mitochondria. </p></div> | ||
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When the TOC and TIC proteins were successfully assembled onto the membranes of mitochondria, basically speaking the goal for building up a new transport machinery is achieved. The TOC and TIC proteins will act in tandem to transport proteins across the two membranes of YAO into the matrix, as long as the proteins to be transported are equipped with the signal peptides that can be recognized by Toc34 (Toc34-targeting signal peptides BioBricks are not available presently).</p></ul> | When the TOC and TIC proteins were successfully assembled onto the membranes of mitochondria, basically speaking the goal for building up a new transport machinery is achieved. The TOC and TIC proteins will act in tandem to transport proteins across the two membranes of YAO into the matrix, as long as the proteins to be transported are equipped with the signal peptides that can be recognized by Toc34 (Toc34-targeting signal peptides BioBricks are not available presently).</p></ul> | ||
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<p>Figure 4. Test of the new transport machinery. 1) Adding signal peptides recognized by TOC & TIC to reporters, and directing towards TOC & TIC complex. 2) Reporters transported into mitochondrial matrix. 3) Removal of the original TOM & TIM complex. </p></div> | <p>Figure 4. Test of the new transport machinery. 1) Adding signal peptides recognized by TOC & TIC to reporters, and directing towards TOC & TIC complex. 2) Reporters transported into mitochondrial matrix. 3) Removal of the original TOM & TIM complex. </p></div> | ||
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Revision as of 07:43, 25 September 2012