The KlADH4-Promoter from the yeast ''Kluyveromyces lactis'' regulates the expression of a mitochondrial alcohol dehydrogenase in an ethanol-dependent way.
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The upstream activating sequence of the promoter contains the consensus sequence of the of the RAP1 (repressor activator protein 1) binding site as well as 5 stress response elements. Those 6 site are essential to the ethanol inducibility of the promoter and their respective transcription factors are present in ''K. lactis'' as well as ''S. cerevisiae''.(4,6)
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=== Light Switchable Promoter ===
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The idea behind a lightswitchable system is to create a gene expression system which can be induced and deactivated by light of a certain wavelengths.
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This system is extremely attractive, as induction does not require the addition of a specific substance. This makes induction cheap, fast, precise and also compatible to the bavarian purity law.
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=== Constitutive Promoter ===
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When all enzymes are expressed under control of promoters with the same strength, the enzyme with the lowest kinetic rates will cause a bottleneck in the pathway. Hence a multitude of promoters of different strengths is needed.
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