Team:KIT-Kyoto/kazukokekokko
From 2012.igem.org
(Difference between revisions)
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<td width="800px" height="510px" valign="top"> | <td width="800px" height="510px" valign="top"> | ||
<div id="MIGI"> | <div id="MIGI"> | ||
+ | LB medium | ||
+ | <br> | ||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td>Bacto-tryptone</Td><Td>10g</Td></Tr> | ||
+ | <Tr><Td>Bacto-yeast extract</Td><Td>5g</Td></Tr> | ||
+ | <Tr><Td>NaCl</Td><Td>10g</Td></Tr> | ||
+ | <Tr><Td>dH<sub>2</sub>O</Td><Td>1000mL</Td></Tr> | ||
+ | </Table> | ||
+ | <br> | ||
+ | <br> | ||
+ | LB plate | ||
+ | <br> | ||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td>Bacto-tryptone</Td><Td>10g</Td></Tr> | ||
+ | <Tr><Td>Bacto-yeast extract</Td><Td>5g</Td></Tr> | ||
+ | <Tr><Td>NaCl</Td><Td>10g</Td></Tr> | ||
+ | <Tr><Td>dH<sub>2</sub>O</Td><Td>1000mL</Td></Tr> | ||
+ | </Table> | ||
+ | <br> | ||
+ | <br> | ||
+ | Solution Ⅰ | ||
+ | <br> | ||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td>Glucose</Td><Td>50mM</Td></Tr> | ||
+ | <Tr><Td>EDTA</Td><Td>10mM</Td></Tr> | ||
+ | <Tr><Td>Tris-HCl</Td><Td>25mM</Td></Tr> | ||
+ | </Table> | ||
+ | <br> | ||
+ | <br> | ||
+ | Solution Ⅱ | ||
+ | <br> | ||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td>NaOH</Td><Td>2N</Td></Tr> | ||
+ | <Tr><Td>SDS</Td><Td>1%</Td></Tr> | ||
+ | </Table> | ||
+ | <br> | ||
+ | <br> | ||
+ | Solution Ⅲ | ||
+ | <br> | ||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td>K-acetate</Td><Td>5M</Td></Tr> | ||
+ | </Table> | ||
+ | <br> | ||
+ | <br> | ||
BP reaction by invitrogen gateway system | BP reaction by invitrogen gateway system | ||
<Table Border Cellspacing="0"> | <Table Border Cellspacing="0"> | ||
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<Table Border Cellspacing="0"> | <Table Border Cellspacing="0"> | ||
<Tr><Td>BP DNA sample</Td><Td>50-150ng/reaction</Td></Tr> | <Tr><Td>BP DNA sample</Td><Td>50-150ng/reaction</Td></Tr> | ||
- | < | + | <tr><td>Destination vector</td><td>150ng/reaction</td></tr> |
<Tr><Td>TE Buffer</Td><Td>up</Td></Tr> | <Tr><Td>TE Buffer</Td><Td>up</Td></Tr> | ||
<Tr><Td>Total</Td><Td>8uL or 9uL</Td></Tr> | <Tr><Td>Total</Td><Td>8uL or 9uL</Td></Tr> |
Revision as of 11:07, 10 September 2012
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LB medium
LB plate
Solution Ⅰ
Solution Ⅱ
Solution Ⅲ
BP reaction by invitrogen gateway system
LR reaction by invitrogen gateway system
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