Team:Tianjin/Notebook
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(→Sept. 18th, 2012) |
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+ | <div id="secondpane"> <!--Code for menu starts here--> | ||
+ | <p class="menu_head">Notebook Contents</p> | ||
+ | <div class="menu_body"> | ||
+ | <div class="menu_children"> | ||
+ | <a href="https://2012.igem.org/Team:Tianjin/Notebook/Modeling">Modeling Notebook</a> | ||
+ | </div> | ||
+ | <div class="menu_children"> | ||
+ | <a href="https://2012.igem.org/Team:Tianjin/Notebook/HumanPractice">Human Practice Notebook</a> | ||
+ | </div> | ||
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+ | <p class="menu_head">July, 2012</p> | ||
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+ | </div> | ||
+ | <p class="menu_head">August, 2012</p> | ||
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+ | <p class="menu_head">September, 2012</p> | ||
+ | <div class="menu_body"> | ||
+ | <div class="Calender" style="height:187px;"> | ||
+ | <div class="Calender-non">Sun | ||
+ | </div> | ||
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<center><span style="font-size:46px;font-family:Cambria;margin-top:10px;line-height:80%">Notes of Experiments</span></center> | <center><span style="font-size:46px;font-family:Cambria;margin-top:10px;line-height:80%">Notes of Experiments</span></center> | ||
==July 6th, 2012== | ==July 6th, 2012== | ||
Line 24: | Line 406: | ||
##3M KOAc / 2M HOAc | ##3M KOAc / 2M HOAc | ||
#Experiment technologies training, such as making gel, gel electrophoresis. | #Experiment technologies training, such as making gel, gel electrophoresis. | ||
- | [[File:TJU2012-Note-gel-1.png|center| | + | [[File:TJU2012-Note-gel-1.png|center|350px|gel 1]] |
- | [[File:TJU2012-Note-gel-2.png|center| | + | |
+ | |||
+ | [[File:TJU2012-Note-gel-2.png|center|350px|gel 2]] | ||
==July 11th, 2012== | ==July 11th, 2012== | ||
Line 33: | Line 417: | ||
##PCR principle and operation | ##PCR principle and operation | ||
##Gel Extraction | ##Gel Extraction | ||
- | [[File:TJU2012-Note-gel-3.png|center| | + | [[File:TJU2012-Note-gel-3.png|center|350px|gel 3]] |
- | [[File:TJU2012-Note-gel-4.png|center| | + | |
+ | |||
+ | [[File:TJU2012-Note-gel-4.png|center|350px|gel 4]] | ||
==July 13th, 2012== | ==July 13th, 2012== | ||
1. Received the oligo and began to mutate 16S rrnB operator. | 1. Received the oligo and began to mutate 16S rrnB operator. | ||
- | [[File:TJU2012-Note-gen-1.png|center| | + | [[File:TJU2012-Note-gen-1.png|center|350px|Gene1]] |
2. Mutated RBS of RFP and checked through gel electrophoresis. | 2. Mutated RBS of RFP and checked through gel electrophoresis. | ||
- | [[File:TJU2012-Note-gen-2.png|center| | + | [[File:TJU2012-Note-gen-2.png|center|250px|Gene2]] |
- | [[File:TJU2012-Note-gel-5.png|center| | + | [[File:TJU2012-Note-gel-5.png|thumb|center|250px|~1800bp, constant with anticipation]] |
==July 14th, 2012== | ==July 14th, 2012== | ||
+ | #Transform two plasmids of we got yestday together into E.coli.[[File:TJU2012-Note-gen-3.png|center|500px|Gene 3]] | ||
+ | #PCR verification of colonies on the LB plates. | ||
+ | #Inculcated the right colonies in Liquid LB. | ||
+ | |||
==July 15th, 2012== | ==July 15th, 2012== | ||
+ | #Extract plasmid of cells inculcated after one day. | ||
+ | #Enzyme test of the extracted plasmid.[[File:TJU2012-Note-gel-6.png|center|200px|Gel 6]] | ||
+ | #Inclucated the right cells in Liquid LB and added Ala partly. | ||
+ | |||
==July 20th, 2012== | ==July 20th, 2012== | ||
+ | Analyse the results. | ||
+ | [[File:TJU2012-Note-form-1.png|center|500px|Form 1]] | ||
+ | [[File:TJU2012-Note-fig-2.png|center|300px|Fig 2]] | ||
+ | |||
==July 24th, 2012== | ==July 24th, 2012== | ||
+ | #Linked the GRP gene and O-RBS RFP gene. Gel extract the previous product again, and ligate using T4 with the following gradient.[[File:TJU2012-Note-form-2.png|center|500px|form 2]] | ||
+ | #In the same method to construct three other systems. | ||
+ | [[File:TJU2012-Mode-cal-fig-2.png|center|500px|fig 3]] | ||
+ | [[File:TJU2012-Mode-cal-fig-3.png|center|500px|fig 4]] | ||
+ | [[File:TJU2012-Mode-cal-fig-4.png|center|500px|fig 5]] | ||
+ | |||
==July 30th, 2012== | ==July 30th, 2012== | ||
+ | #Learned Fluorescence spectrophotometer. | ||
+ | #measure the strength of GFP and RFP. | ||
+ | [[File:TJU2012-Note-form-3.png|center|500px|form 3]] | ||
+ | |||
==Aug. 2nd, 2012== | ==Aug. 2nd, 2012== | ||
+ | #mutated the RBS of the Amp resistance gene.[[File:TJU2012-Note-gen-4.png|center|250px|Gene 4]] | ||
+ | #linked genes and transformed them into E. coli. | ||
+ | |||
==Aug. 4th, 2012== | ==Aug. 4th, 2012== | ||
+ | Results of plates | ||
+ | [[File:TJU2012-Note-fig-3.png|center|300px|fig 3]] | ||
+ | [[File:TJU2012-Note-form-4.png|center|500px|form 4]] | ||
+ | |||
==Aug. 8th, 2012== | ==Aug. 8th, 2012== | ||
+ | Began to construct the five parts needed in Assembler in Yeast. Part 1 and part 2. | ||
+ | |||
==Aug. 11th, 2012== | ==Aug. 11th, 2012== | ||
+ | Verified part 1 and 2. Synthesized the two small parts and linked through overlap PCR. Verified through ligase digestion and then gel electrophoresis. | ||
+ | [[File:TJU2012-Note-fig-4.png|center|600px|fig 4]] | ||
+ | |||
+ | |||
+ | [[File:TJU2012-Note-fig-5.png|center|500px|fig 5]] | ||
+ | |||
==Aug. 12th, 2012== | ==Aug. 12th, 2012== | ||
+ | Began to build part 3, 4 and 5. Synthesized the two small parts and linked through overlap PCR. | ||
+ | |||
==Aug. 14th, 2012== | ==Aug. 14th, 2012== | ||
- | ==Aug. | + | Verify part 3, 4 and 5. Verified through ligase digestion and then gel electrophoresis. |
+ | [[File:TJU2012-Note-fig-6.png|center|500px|fig 6]] | ||
+ | |||
+ | |||
+ | [[File:TJU2012-Note-fig-7.png|center|500px|fig 7]] | ||
+ | |||
+ | |||
+ | [[File:TJU2012-Note-fig-8.png|center|500px|fig 8]] | ||
+ | |||
+ | ==Aug. 18th, 2012== | ||
+ | Transformed all the five parts into Yeast for Assembler in Yeast. | ||
+ | |||
==Aug. 20th, 2012== | ==Aug. 20th, 2012== | ||
- | ==Aug. | + | #Results of the transformation.[[File:TJU2012-Note-fig-9.png|center|300px|fig 9]] |
+ | #Extract plasmids from the Yeast. | ||
+ | |||
+ | ==Aug. 22nd, 2012== | ||
+ | #Transformed the plasmid from the Yeast into cells.[[File:TJU2012-Note-fig-10.png|thumb|center|300px|There are purple spots on the plates]] | ||
+ | #Extracted plasmid and checked through gel electrophoresis.[[File:TJU2012-Note-gel-7.png|center|300px|gel 7]] | ||
+ | #Some other results of Assembler in Yeast. | ||
+ | [[File:TJU2012-Note-fig-11.png|center|300px|fig 11]] | ||
+ | |||
+ | |||
+ | [[File:TJU2012-Note-fig-12.png|center|300px|fig 12]] | ||
+ | |||
+ | |||
+ | [[File:TJU2012-Note-fig-13.png|center|300px|fig 13]] | ||
+ | |||
+ | |||
+ | [[File:TJU2012-Note-fig-14.png|center|300px|fig 14]] | ||
+ | |||
==Aug. 25th, 2012== | ==Aug. 25th, 2012== | ||
+ | #Began to build biobricks.[[File:Tianjin_BB2.PNG|center|500px|BB 2]] | ||
+ | #Began to search articles on phages. | ||
+ | |||
==Sept. 1st, 2012== | ==Sept. 1st, 2012== | ||
+ | Went to Peking University for exchanges. | ||
+ | [[File:TJU2012-Note-fig-15.png|center|500px|fig 15]] | ||
+ | |||
+ | |||
+ | [[File:TJU2012-Note-fig-16.png|center|500px|fig 16]] | ||
+ | |||
==Sept. 2nd, 2012== | ==Sept. 2nd, 2012== | ||
- | ==Sept. | + | #Found out phi X174 and firstly proposed our ideas. |
- | ==Sept. | + | #The second sections of our biobricks. |
- | ==Sept. | + | [[File:Tianjin_BB1.PNG|center|500px|BB 1]] |
+ | |||
+ | ==Sept. 5th, 2012== | ||
+ | #Some experiments on phages. | ||
+ | #Optimized our biobricks. | ||
+ | #Designed primers needed for mutating Phi X174 and send orders. | ||
+ | |||
+ | ==Sept. 11th, 2012== | ||
+ | #Began to mutate gene G and E. | ||
+ | #Optimized our biobricks. | ||
+ | |||
+ | ==Sept. 18th, 2012== | ||
+ | Began to sort out our materials for wiki and upload the website. | ||
+ | [[File:new1.jpg|center|500px|fig 17]] | ||
+ | our team in Hongkong | ||
+ | |||
+ | |||
+ | [[File:new2.jpg|center|500px|fig 18]] | ||
+ | our gold award | ||
+ | |||
+ | |||
+ | [[File:new3.jpg|center|500px|fig 19]] | ||
+ | presentation in MIT | ||
{{:Team:Tianjin/footer}} | {{:Team:Tianjin/footer}} |
Latest revision as of 05:16, 9 May 2013
July 6th, 2012
- We discuss our all of the possible projects, and finally choose one. And then allocate works to specific member.
- Do some pre-experiment and make some reagants.
- Liquid LB
- 10g tryptone
- 5g yeast extract
- 10g NaCl
- Solid LB
- 10g tryptone
- 5g yeast extract
- 10g NaCl
- 20g agar
- Liquid LB
- Cleaned up the lab and laboratory instruments
July 8th, 2012
- Solutions for extracting plasmid.
- 50mM Glucose / 25mM Tris-Cl / 10mM EDTA,pH=8.0
- 0.2N NaOH / 1% SDS
- 3M KOAc / 2M HOAc
- Experiment technologies training, such as making gel, gel electrophoresis.
July 11th, 2012
- Designed primers and sent orders.
- Optimized the project and allocated work.
- Experiment technologies and knowledge training
- PCR principle and operation
- Gel Extraction
July 13th, 2012
1. Received the oligo and began to mutate 16S rrnB operator.
2. Mutated RBS of RFP and checked through gel electrophoresis.
July 14th, 2012
- Transform two plasmids of we got yestday together into E.coli.
- PCR verification of colonies on the LB plates.
- Inculcated the right colonies in Liquid LB.
July 15th, 2012
- Extract plasmid of cells inculcated after one day.
- Enzyme test of the extracted plasmid.
- Inclucated the right cells in Liquid LB and added Ala partly.
July 20th, 2012
Analyse the results.
July 24th, 2012
- Linked the GRP gene and O-RBS RFP gene. Gel extract the previous product again, and ligate using T4 with the following gradient.
- In the same method to construct three other systems.
July 30th, 2012
- Learned Fluorescence spectrophotometer.
- measure the strength of GFP and RFP.
Aug. 2nd, 2012
- mutated the RBS of the Amp resistance gene.
- linked genes and transformed them into E. coli.
Aug. 4th, 2012
Results of plates
Aug. 8th, 2012
Began to construct the five parts needed in Assembler in Yeast. Part 1 and part 2.
Aug. 11th, 2012
Verified part 1 and 2. Synthesized the two small parts and linked through overlap PCR. Verified through ligase digestion and then gel electrophoresis.
Aug. 12th, 2012
Began to build part 3, 4 and 5. Synthesized the two small parts and linked through overlap PCR.
Aug. 14th, 2012
Verify part 3, 4 and 5. Verified through ligase digestion and then gel electrophoresis.
Aug. 18th, 2012
Transformed all the five parts into Yeast for Assembler in Yeast.
Aug. 20th, 2012
- Results of the transformation.
- Extract plasmids from the Yeast.
Aug. 22nd, 2012
- Transformed the plasmid from the Yeast into cells.
- Extracted plasmid and checked through gel electrophoresis.
- Some other results of Assembler in Yeast.
Aug. 25th, 2012
- Began to build biobricks.
- Began to search articles on phages.
Sept. 1st, 2012
Went to Peking University for exchanges.
Sept. 2nd, 2012
- Found out phi X174 and firstly proposed our ideas.
- The second sections of our biobricks.
Sept. 5th, 2012
- Some experiments on phages.
- Optimized our biobricks.
- Designed primers needed for mutating Phi X174 and send orders.
Sept. 11th, 2012
- Began to mutate gene G and E.
- Optimized our biobricks.
Sept. 18th, 2012
Began to sort out our materials for wiki and upload the website.
our team in Hongkong
our gold award
presentation in MIT