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| - | {{:Team:EPF-Lausanne/Template/Header}} | + | {{:Team:EPF-Lausanne/Template/Header|project}} |
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| | = Introduction = | | = Introduction = |
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| - | LovTAP-VP16 is the name of the protein our light switch is based on. But it has never been expresed before and, therefore, there is no literature telling us under what conditions it works or whether it works at all. It's a fusion protein between [[Team:EPF-Lausanne/Modeling/lovtap#LovTAP|LovTAP]], which is itself a fusion protein, and a part of [[Team:EPF-Lausanne/Modeling/lovtap#VP16|VP16]], a viral transcription activator.
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| - | In this case we don't want any kind of steric interaction between LovTAP and VP16, since that might alter the functionality of one or both parts and we just want VP16 to be transported by LovTAP. To ensure this, a linker might be needed to physically separate the domains.
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| | == How LovTAP is thought to work == | | == How LovTAP is thought to work == |
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| - | === Allosteric regulation ===
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| - | In a protein, generally an enzyme, an allosteric site is any part of the protein other than the active site.
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| - | Allosteric regulation of a protein consists in modifying its properties by interacting with an allosteric site.
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| - | One example would be the regulation in the tryptophan (trp) operon,
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| - | a group of genes studied in E. coli that are required for the synthesis of the amino acid tryptophan.
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| - | The expression of these genes can be blocked by the homodimeric protein tryptophan repressor
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| - | (TrpR), by binding the operator of the operon. TrpR repressing function is only active when
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| - | tryptophan is bound to its allosteric sites, i.e. it blocks the production of tryptophan when the
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| - | concentration of tryptophan is high.
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| - | === LovTAP ===
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| - | In a paper published in 2008 [http://www.pnas.org/content/105/31/10709.abstract], Strickland et al. propose to modify the protein TrpR such that
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| - | its activity can be controlled by light. This is done by fusing it to a light sensitive protein, the
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| - | plant phototropin LOV2 (Light-Oxygen-Voltage), whose sensitivity to blue light is conferred by the
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| - | ligand chromophore flavin mononucleotide (FMN). The fusion is done in a way that both domains
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| - | share a common α-helix, which would create a sort of lever that could transfer the conformational
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| - | changes in LOV2 when light-activated towards TrpR, triggering its activation.
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| - | ==== VP16 ====
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| - | [http://pubs.acs.org/doi/full/10.1021/bi0482912|Paper] stating that the part of VP16 we used (456-490) behaves as transcriptional activator.
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| | {{:Team:EPF-Lausanne/Template/Footer}} | | {{:Team:EPF-Lausanne/Template/Footer}} |
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