Search results

Create the page "DNA Submission" on this wiki!

• Team:Cornell/testing/notebook/wetlab/3
  ...nd internal cut site (we expect lower mutation efficiency because template DNA is not methylated). Second, we transformed DH5a with the mutated plasmid. T ...cut site within the nah operon) worked. Unfortunately, there wasn't enough DNA to see anything when visualized on a gel. They decided to proceed with elec
  105 KB (17,618 words) - 03:59, 4 October 2012
• Team:Cornell/notebook/wetlab/august
  ...nd internal cut site (we expect lower mutation efficiency because template DNA is not methylated). Second, we transformed DH5a with the mutated plasmid. T ...cut site within the nah operon) worked. Unfortunately, there wasn't enough DNA to see anything when visualized on a gel. They decided to proceed with elec
  125 KB (19,514 words) - 22:59, 26 October 2012
• Team:KIT-Kyoto/Notebook-week4p
  <Tr><Td>　DNA sample　</Td><Td>　1uL　</Td></Tr> <Tr><Td>　DNA sample　</Td><Td>　1uL　</Td></Tr>
  15 KB (2,364 words) - 13:32, 26 September 2012
• Team:Calgary/Notebook/Hydrocarbon
  ...oolkit' were a restriction digest protocol, PCR purification, and finally, DNA construction digest. Aims moving forward include obtaining strains of the < ...ed by nanodrop to confirm successful plasmid extraction, and the resulting DNA concentrations were as follows: </p>
  101 KB (16,854 words) - 18:44, 21 September 2012
• Team:Calgary/Notebook/Denitrogenation
  ... these cultures, and plan on attempting to amplify genes from the isolated DNA next week. </p> ...3.5 ng/microlitre of <i>antB</i> DNA and 129 ng/microlitre of <i>carAc </i>DNA. These concentrations were both sufficient to begin a restriction digest an
  41 KB (7,115 words) - 00:16, 27 October 2012
• Regions/Europe/Pre-Jamboree
  <li><a href="#13">DNA submission</a></li> ...s://2012.igem.org/Regions/Europe/Transnatural">here</a> the details of the submission.
  9 KB (1,518 words) - 08:39, 22 September 2012
• Team:BostonU/Notebook
  ... with DVL0_ER (SJ4). We performed Gel Electrophoresis on this set of DNA.</li> ...protocol. We then quantified the amount of DNA we had using NanoDrop DNA quantification protocol andset up a restriction digest (SpeI, BSA, Bu
  46 KB (7,749 words) - 17:47, 26 October 2012
• Regions/Asia/Jamboree/Pre-regional Jamboree
  <li><a href="#13">DNA Submissions</a></li> ... this<a href="https://igem.org/Safety"> page </a> for more information.The submission due date is <strong>September 7th</strong>.
  24 KB (3,849 words) - 02:43, 4 October 2012
• Regions/Asia/pre-jamboree
  ... this<a href="https://igem.org/Safety"> page </a> for more information.The submission due date is <strong>September 7th</strong>. ... <a href="https://2012.igem.org/Safety">page</a> for more information. The submission due date will be on <strong>September 7th</strong>.
  18 KB (2,899 words) - 03:19, 22 August 2012
• Main Page Archive
  ...artsregistry.org/wiki/index.php/DNA_Submission_Instructions">BioBrick part DNA due to Registry</a></li> <li><i>submission <a href="https://2012.igem.org/Calendar_of_Events">deadlines</a> are coming
  70 KB (11,003 words) - 20:59, 14 November 2012
• Team:Calgary/Notebook/Desulfurization
  ...oolkit' were a restriction digest protocol, PCR purification, and finally, DNA construction digest. Aims moving forward include obtaining strains of the < ...tures, which were then miniprepped the following day to obtain the plasmid DNA of the putative <i>hpaC</i> biobrick. Digestions were performed on the mini
  65 KB (10,832 words) - 16:10, 3 September 2013
• Team:UNITN-Trento/Notebook
  ...roduct on the gel . I proceed with the restriction of CysE and pSB1C3 (the submission vector) with E and P, and then ligation and transformation in Nova Blue cel ...time we used the registry protocol; This time we used higher quantities of DNA. For the insert we used PCR products rather than a plasmid.<br/>
  82 KB (13,657 words) - 08:27, 24 September 2012
• Team:Cambridge/Overview/Labbook
  ...on of DNA = 360ng/&mu;l, so 2&mu;l needed for the desired 0.6&mu;g of DNA. DNA added to bacteria. *Nanodrop was used to determine the concentration of DNA in the biobrick after resuspension. The result reading was 103ng/ul.
  14 KB (2,109 words) - 02:36, 27 October 2012
• Team:TU Munich/Notebook/Meetings
  ...wn to work by a different research lab. It would be a good idea to use the DNA they submitted to the registry and make it functional (e.g. removing the fo *How do we get the physical DNA (ask a research lab to send it to us, extract from organisms via PCR, synth
  103 KB (15,460 words) - 13:23, 26 September 2012
• Team:St Andrews/Notebook
  ...Very quickly, "BioSilta", "GenScript", "Clontech", "Geneious", "Integrated DNA Technologies" and "Thermo Fisher" pledge their support and Team St Andrews ...ope" are all well received. Children and adults alike are fascinated when DNA is extracted from bananas, using everyday kitchen utensils, before their ey
  27 KB (4,526 words) - 02:47, 27 September 2012
• Requirements
  <li><strong>Part Submission:</strong> ...href="http://partsregistry.org/wiki/index.php/DNA_Submission_Instructions">submission instructions</a></li>
  5 KB (725 words) - 18:49, 4 September 2012
• Team:WashU/Week12
  We also PCR'd the Z construct in the submission plasmid, Psb1c3. We then digested the results with both EcoRI and PstI, as ...llowed by ligation of Z + TOPO, U + TOPO, and our main construct CS42S for submission.
  5 KB (772 words) - 02:26, 15 September 2012
• Team:UC Davis/Notebook/Protocols
  <li>DNA template</li> <li>T4 DNA Ligase
  49 KB (6,768 words) - 02:52, 27 October 2012
• World Championship Jamboree/Handbook
  ====DNA Submissions==== ...mission | DNA submission requirements]] for more information about the DNA submission process.
  35 KB (5,686 words) - 18:00, 23 October 2012
• Team:Rutgers/BEAS
  ..., LovTAP is a fusion protein. It consists of a light-response domain and a DNA binding domain, each of which are parts of other natural proteins.</p> <h4 class="shadow">DNA Binding</h4>
  12 KB (1,704 words) - 04:06, 4 October 2012