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Create the page "10% DMSO stocks" on this wiki!

• Team:TU Munich/Notebook/Labjournal
  <a href="#Week_10">Week 10</a> 13.8-19.8<br> <a href="#Week_14">Week 14</a> 10.9-16.9<br>
  793 KB (114,680 words) - 14:38, 3 May 2013
• Team:Potsdam Bioware/Lab/Labjournal/September
  Transformation via manual, 10 µl of miniprep sample and 1 µL of pCEP4 were used * vortex for 10 s 3 x
  236 KB (34,819 words) - 22:05, 26 October 2012
• Team:Bielefeld-Germany/Labjournal
  <li><a href="#10"><strong>Week 9</strong></a></li> <li><a href="#11"><strong>Week 10</strong></a></li>
  333 KB (50,931 words) - 03:59, 27 October 2012
• Team:MIT/Notebook
  ...24 G, 224 PI-SceI, started gel at 100V at 9:40, run for 25 mins, finish at 10:05</td></tr> ...ments">Inoculated culture 1 from -80C iGem box using 1000:1 Amp (10 ul for 10 ml), split into 2 x 5ml tubes. Put in 37 C shaker at 9:00 PM</td></tr>
  76 KB (12,442 words) - 22:58, 26 October 2012
• Team:British Columbia/ConsortiaNotebook
  <area shape="rect" coords="10,5,220,70" href="https://2012.igem.org/Team:British_Columbia/LabNotebook" /> <area shape="rect" coords="10,90,220,155" href="https://2012.igem.org/Team:British_Columbia/ConsortiaNote
  53 KB (9,047 words) - 06:59, 28 September 2012
• Team:UIUC-Illinois/Notebook/LabNotebook
  <li><a name="week10puf" >Week 10</a></li> <td>- Made glycerol stocks of C0012, E0030, I732017, PSB1A3, PSB1AK3, PSB1K3 and put them in the -80 f
  59 KB (9,788 words) - 05:14, 26 October 2012
• Team:British Columbia/BiobrickConstruction
  <area shape="rect" coords="10,5,220,70" href="https://2012.igem.org/Team:British_Columbia/LabNotebook" /> <area shape="rect" coords="10,90,220,155" href="https://2012.igem.org/Team:British_Columbia/BiobrickConst
  30 KB (5,082 words) - 03:34, 4 October 2012
• Team:TU Darmstadt/Labjournal/Degradation
  * 2-5 1.PCR, 6-9 2.PCR, 10-13 3.PCR ** c(Promo-LacO-RBS-Phoa-FsC)=10 ng/µL
  139 KB (19,958 words) - 02:51, 27 September 2012
• Team:NTNU Trondheim/Notebook
  ...ned in the notebook, see [https://2012.igem.org/Team:NTNU_Trondheim/Stocks Stocks] and [https://2012.igem.org/Team:NTNU_Trondheim/Constructs Constructs]. For ...protocols]. The concentration after gel extraction was: c_plld = 10,6 ng/µl.
  95 KB (15,220 words) - 17:15, 25 September 2012
• Team:NTNU Trondheim/Test/Notebook
  ...ned in the notebook, see [https://2012.igem.org/Team:NTNU_Trondheim/Stocks Stocks] and [https://2012.igem.org/Team:NTNU_Trondheim/Constructs Constructs]. For ...protocols]. The concentration after gel extraction was: c_plld = 10,6 ng/µl.
  95 KB (15,213 words) - 09:43, 21 September 2012
• Team:Grenoble/Biology/Notebook/August
  <li><b>Lane 2:</b> pSB4C5 (miniprep) PCR product (DMSO)</li> <li><b>Lane 3:</b> pSB4C5 (miniprep) PCR product (DMSO)</li>
  14 KB (2,258 words) - 10:07, 26 September 2012
• Team:NTNU Trondheim/Notebook/July
  ...e culture with nitrogen gas to remove oxygen. Samples were collected every 10 minutes. Updated information about stored DNA samples and glycerol stocks on the wiki.
  51 KB (7,919 words) - 17:23, 25 September 2012
• Team:Grenoble/Biology/Notebook/July/week 30
  ...FP, and on colony to amplify RBS_Cya. We did the PCR both with and without DMSO.<br/> <li><b>Lane 2:</b> pSB4K5 PCR product (DMSO)</li>
  15 KB (2,516 words) - 14:04, 26 September 2012
• Team:Stanford-Brown/Protocols
  -We have 50x stocks of BG11 in the fridge that are or can be aliquoted into 20mL samples '''Antibiotic Stocks'''
  34 KB (5,685 words) - 22:10, 3 October 2012
• Team:Wisconsin-Madison/protocol
  <li>10 mM EDTA (pH 8.0)</li> Prepare Solution I from standard stocks in batches of -100 ml, autoclave for 15 minutes at 15 psi (l.05 kg/cm2 ) on
  42 KB (7,189 words) - 18:05, 3 October 2012
• Team:MIT/MaterialsAndMethods
  ...omponents to a 1.5 mL tube at room temperature and mix: attB-PCR product (=10 ng/uL; final amount ~15-150 ng) 1-7 uL , Donor vector (150 ng/uL) 1 uL ,TE ...o terminate the reaction. Vortex briefly. Incubate samples at 37&deg;C for 10 minutes. </LI>
  42 KB (6,746 words) - 03:12, 4 October 2012
• Team:TU Darmstadt/Protocols/Metabolism
  * Inoculate 10 ml of LB-Media with an E. coli colony and incubate at 37 °C overnight * Centrifuge the cells for 10 min at 5000 rpm and 4 °C
  17 KB (2,545 words) - 23:00, 26 September 2012
• Team:Calgary/Notebook/Hydrocarbon
  ...2011 (see https://2011.igem.org/Team:Washington/alkanebiosynthesis). These stocks are as follows: </p> <li> 10 mL of 1 mg/mL Thiamine </li>
  101 KB (16,854 words) - 18:44, 21 September 2012
• Team:UNITN-Trento/Protocols
  filter:blur(add=false, direction=270 strength=10); -ms-filter:blur(add=false, direction=270 strength=10);
  45 KB (6,225 words) - 11:07, 25 September 2012
• Team:Columbia-Cooper-NYC/Columbia notebook 2
  * Relocated and reorganized the iGEM biobrick kit and glycerol stocks * Added 10µl of ho1 and PcyA to 5ml of antibiotics
  37 KB (5,174 words) - 07:36, 8 January 2013