Team:Tsinghua-A/Wetlab

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<h2 id="titleText" style="color:rgb(133,212,246)">Tsinghua-A::<span style="color: rgb(0,118,167);">Wetlab</span></h2>
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     <h2 class="textTitle" >Prokaryotic cells</h2>
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<p>'Life' is too big a word, especially when we are talking about synthetic biology, but we still find it interesting, in our life, when everyone meets alternatives and makes his decisions, that colorful behaviors and lifestyles just differentiate from small left-or-right choices, and that widely-differed ones make up a well-operated society. This year our study will be concentrated on controllable choice-making E. Coli, with left-or-right directions of gene expression to be determined, thus to realize the life-like differentiation and dedifferentiation, and synthesized function of the cell 'teamwork'. That's the idea why we named our project after 'E. CoLIFE'.<a class="textLink" href="www.baidu.com">readmore</a></p>
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<p>In prokaryotic cells, the system consists of three circuits. One induces flip, one flips and the other expresses. According to the properties of the promoter pBAD, we build the first circuit, using arabinose as an inducement to generate the recombinase Cre.<a class="textLink" href="https://2012.igem.org/Team:Tsinghua-A/Wetlab/Part1">readmore</a></p>
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<p>'Life' is too big a word, especially when we are talking about synthetic biology, but we still find it interesting, in our life, when everyone meets alternatives and makes his decisions, that colorful behaviors and lifestyles just differentiate from small left-or-right choices, and that widely-differed ones make up a well-operated society. This year our study will be concentrated on controllable choice-making E. Coli, with left-or-right directions of gene expression to be determined, thus to realize the life-like differentiation and dedifferentiation, and synthesized function of the cell 'teamwork'. That's the idea why we named our project after 'E. CoLIFE'.</p>
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<p>Life process in eukaryotic cells is much more complicate, especially when it is related to signal transportation. We choose microRNA to transport cues and Bxb1 to realize the flipping process. Using microRNA can not only simplify the design of the system, making sure the transportation is precise and efficient, but also ensure the independency with other life processes due to the orthogonality of the microRNA and other signals. <a class="textLink" href="https://2012.igem.org/Team:Tsinghua-A/Wetlab/Part2">readmore</a></p>
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    <h2 class="textTitle">Optimization</h2>
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<p>The efficiency of cre-loxp system is extremely vital to our design; it may fluctuate a lot in that the DNA are reversible.<br/>
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Based on our model, we designed some experiments to test the inversion efficiency. We used the flow cytometer to analyz our results.<a class="textLink" href="https://2012.igem.org/Team:Tsinghua-A/Wetlab/Optimization">readmore</a></p>
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    <h2 class="textTitle">Parts</h2>
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<p>We constructed 4 parts and more parts are under construction.<a class="textLink" href="https://2012.igem.org/Team:Tsinghua-A/Wetlab/Part3">readmore</a></p>
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                <a class="navigationButtonA" href="#" style="color:rgb(133,212,246); ">Prokaryotic cells</a></li>
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                <a class="navigationButtonA" href="#2" style="color:rgb(133,212,246);">Eukaryotic cells</a></li>
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                <a class="navigationButtonA" href="#4" style="color:rgb(133,212,246)">Optimization</a></li>
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Latest revision as of 20:05, 26 October 2012

Tsinghua-A::Wetlab

Prokaryotic cells

In prokaryotic cells, the system consists of three circuits. One induces flip, one flips and the other expresses. According to the properties of the promoter pBAD, we build the first circuit, using arabinose as an inducement to generate the recombinase Cre.readmore

Eukaryotic cells

Life process in eukaryotic cells is much more complicate, especially when it is related to signal transportation. We choose microRNA to transport cues and Bxb1 to realize the flipping process. Using microRNA can not only simplify the design of the system, making sure the transportation is precise and efficient, but also ensure the independency with other life processes due to the orthogonality of the microRNA and other signals. readmore

Optimization

The efficiency of cre-loxp system is extremely vital to our design; it may fluctuate a lot in that the DNA are reversible.
Based on our model, we designed some experiments to test the inversion efficiency. We used the flow cytometer to analyz our results.readmore

Parts

We constructed 4 parts and more parts are under construction.readmore

Retrieved from "http://2012.igem.org/Team:Tsinghua-A/Wetlab"