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Contents 1 What's a YES gate? 2 YES gate #1 3 YES gate #2 4 YES gate #3

What's a YES gate?

A yes gate is one of the simplest molecular logic gate operations. The truth table for a YES gate is:

Input	Output
1	1
0	0

The YES gates in our project consisted of an inducer which triggered the expression from a dedicated promoter. Consequentially, a fluorescent protein and an RNA polymerase are produced. The general scheme of a YES gate is presented in figure 1.

When planing a complex system, there is no way of knowing which combination of promoter and RNAP will work well. Therefore, we planned to attempt the cloning of different RNAPs under the various promoters.
The choice of FPs to follow each promoter was not random. The different considerations will be described below.

YES gate #1

The first YES gate consists of a P_TetO promoter which is repressed by TetR. The expression is induced by anhydrotetracycline (aTc). At first, we attempted the cloning of mCitrine downstream to P_TetO in plasmids we got from Roee. These showed no fluorescence when tested in the plate reader. Therefore, we turned to the registry for alternatives. We found BBa_I13522 and I13600 which express P_TetO+GFP and P_TetO+CFP respectively.
Continue the tale of failed clones and tell about P_TetO+mCherry.

YES gate #2

The second YES gate consists of P_Lac/Ara promoter which is repressed by

YES gate #3