Team:ETH Zurich/Project

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Contents

Overview

UVR8-TetRDBD

Binding of TetR to the tetO

We implement a novel two-hybrid screening for E.Coli using the TetR-DNA binding domain. TetRDBD on its own is unable to bind to the Tet-Promoter. After fusing it in different ways to UVR8 - a UV-B-detecting-protein from plants - repression occured.


LovTAP

LovTap is a fusion of a photoreceptor with a transcriptional regulator TrpR. This fusion protein can be activated by blue light and therefore act as a transcriptional switch. We want to use this system for the UV intensity measurement and as an input for our decoder.


Cph8

Cph8 consists of the light-receptor Cph1 that is deactivated by red light and the histidine kinase EnvZ responsible for the downstream signaling.


Decoder

Boolean logic of Decoder

Our decoder aims the combination of blue and red light to distinguish different lightsources like sunlight or room light. For this we use LovTAP and Cph8 and perform the calculation with the help of three NOR gates each represented by a hybrid promoter.



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