Team:Calgary/Notebook/Protocols

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<h2>General Protocols</h2>
<h2>General Protocols</h2>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/agarosegel">Agarose Gel Electrophresis</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/agarosegel">Agarose Gel Electrophresis</a></li>
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<li><a href="">Bacterial Genomic DNA Purification</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/gemomicprep">Bacterial Genomic DNA Purification</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/transformation">Bacterial Transformation</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/transformation">Bacterial Transformation</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/construction">Construction Techniques</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/construction">Construction Techniques</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Carbazole GC-MS Analysis">GC-MS Analysis</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/gelextraction">Gel Extraction</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/gelextraction">Gel Extraction</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Gibson Assembly">Gibson Assembly</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/lbagar">LB Agar Plates</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/lbagar">LB Agar Plates</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/m9media">M9 Minimal Media Preparation</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/oextraction">Organic Extraction</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/onculture">Overnight Cultures</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/onculture">Overnight Cultures</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/pcrpurification">PCR Purification</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/pcrpurification">PCR Purification</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/picogreen">PicoGreen Assay</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/plasmidminiprep">Plasmid Purification (from <i>E. coli</i>)</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/plasmidminiprep">Plasmid Purification (from <i>E. coli</i>)</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/compcells">Preparing Chemically Competent Cells (<i>E. coli</i>)</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/compcells">Preparing Chemically Competent Cells (<i>E. coli</i>)</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/glycerolstock">Preparing Glycerol Stocks (<i>E. coli</i>)
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/glycerolstock">Preparing Glycerol Stocks (<i>E. coli</i>)
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dnarehydration">Rehydration of Registry DNA</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dnarehydration">Rehydration of Registry DNA</a></li>
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<li><a href="">Site-Directed Mutagenesis</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dmszfreezedry">Reviving Freeze-dried Bacterial Cultures from DSMZ</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/taqpcr">Taq PCR Protocol</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mutagenesis">Site-Directed Mutagenesis</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/soe">Splice Overlap Extension PCR (SOE PCR)</a></li>
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<h2>Electrochemistry Protocols</h2>
<h2>Electrochemistry Protocols</h2>
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<li><a href="l">Electorchemistry protocols (include agarose bead making) </a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/cvs">Cyclic Voltammetry</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/potstd">Potentiostatic Standard Curve Generation</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/potentiostatic">Reporter Expression Detection</a></li>
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<h2>Desulfurization</h2>
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<h2>Desulfurization Protocols</h2>
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<li><a href="">Desulphuirzation assay protocol</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/catalase">Catalase assay</a></li>
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/catalase">Catalase assay</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/hpac">HPAC assay</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/desulfur">Desulfurization Assay</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/hpac">HpaC assay</a></li>
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<h2>Decarboxylation Protocols</h2>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/PetroBrick Validation Assay">PetroBrick Validation Assay </a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/oleT in  Validation Assay">oleT Validation Assay </a></li>
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<h2>Denitrogenation Protocols</h2>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay"><i>AmdA</i> Characterization Assay</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/carbazole">Carbazole Degradation Assay</a></li>
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<h2>Decatecholization Protocols</h2>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/decatecholization">Decatecholization Assay</a></li>
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<h2>Transposon Mutant Library for Toxin Detection</h2>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/tnscreen">Transposon-Mediated Mutant Library Generation</a></li>
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<h2>Kill Switch Protocols</h2>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgcircuit">Characterization of mgtA regulation with GFP </a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgtacircuit">Characterization of mgtA regulation with S7 killgene </a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Prha Characterization">Characterization of <i>P<sub>rha</sub></i> with GFP</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/escapersassay">Kill Assay for rhamnose system</a></li>
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</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/GlycineAssays">Glycine Auxotrophic Assays (Glycine Media Test, and Petrobrick Test)
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/nucleaseassay">Nuclease assay</a></li>
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</a></li>
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<h2>Bioreactor Assays Protocols</h2>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/bacgrowth">Bacterial Growth Experiments</a></li>
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<p>Belt Selection Tests:</p>
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<ul>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/bactest">Bacteria Test</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/hcseptest">Hydrocarbon Separation Test</a></li><li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/hctest">Hydrocarbon Test</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/hdbeltskim">NA and Hexadecane Belt Skim Test</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/tailingtest">Tailings Test</a></li>
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</ul>
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<h2>Decarboxylation</h2>
 
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<li><a href="">Petrobrick/oleT assay protocols/ GC MS</a></li>
 
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<h2>Denitrogenation</h2>
 
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<h2>Decatecholization</h2>
 
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/decatecholization">Decatecholization (xylE and incubation with the Petrobrick)</a></li>
 
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<h2>Transposon</h2>
 
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<h2>Kill Switch</h2>
 
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<li><a href="">Rhamnose, magnesium and manganese, kill gene assay (nuclease)</a></li>
 
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<h2>Bioreactor</h2>
 
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<li><a href="">Bioreactor assays</a></li>
 
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<h2>Modeling</h2>
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<h2>Modeling Protocols</h2>
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<li><a href="">Modelling validation experiments</a></li>
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Modelvalidation">Modelling validation experiments</a></li>

Latest revision as of 03:54, 27 October 2012

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Protocols

Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.