Producing high yields of B.subtilis spores

Preparation

Plate and grow B. subtilis overnight on antibiotic resistant plates.

Method

• Pick a single colony from a new plate of B. subtilis and use to inoculate 50ml CDSM.
• Grow this for 11 hours in a 37°C incubator with vigorous aeration.
• Transfer 2.5mL of this to 70ml of new CDSM culture. Incubate new CDSM culture and original 50mL CDSM culture for a further 37hrs, to a total of 48hrs.
• Longer incubation may be needed for full release of spores.