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Team:Technion/23 July 2012
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- The ligations and transformations worked. The yield was very small but we still got a few colonies on the plates that were plated with the rest of the cells (after centrifugation).<br> | - The ligations and transformations worked. The yield was very small but we still got a few colonies on the plates that were plated with the rest of the cells (after centrifugation).<br> | ||
- Did colony PCR reactions on 3 colonies from Fus1 HF, 3 colonies from Fus1 GC and 6 colonies of Fus2.<br> | - Did colony PCR reactions on 3 colonies from Fus1 HF, 3 colonies from Fus1 GC and 6 colonies of Fus2.<br> | ||
| - | - Only 1 colony of Fus1 gave positive results and 4 colonies of Fus2 gave positive results.<br> | + | - Only 1 colony of Fus1 gave positive results (#1) and 4 colonies of Fus2 gave positive results (#7, #9, #10, #11).<br> |
| - | - Put starters 10 ml each of the only positive colony of Fus1 and two positive colonies of Fus2. I also left the plate with the isolated colonies in the 37 incubator to grow some more. Hopefully, tomorrow we can miniprep the plasmids and send them for sequencing. | + | - Put starters 10 ml each of the only positive colony of Fus1 and two positive colonies of Fus2 (#7 and #11). I also left the plate with the isolated colonies in the 37 incubator to grow some more. Hopefully, tomorrow we can miniprep the plasmids and send them for sequencing. |
==Inbal== | ==Inbal== | ||
Latest revision as of 16:34, 24 July 2012
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Ilya
- The ligations and transformations worked. The yield was very small but we still got a few colonies on the plates that were plated with the rest of the cells (after centrifugation).
- Did colony PCR reactions on 3 colonies from Fus1 HF, 3 colonies from Fus1 GC and 6 colonies of Fus2.
- Only 1 colony of Fus1 gave positive results (#1) and 4 colonies of Fus2 gave positive results (#7, #9, #10, #11).
- Put starters 10 ml each of the only positive colony of Fus1 and two positive colonies of Fus2 (#7 and #11). I also left the plate with the isolated colonies in the 37 incubator to grow some more. Hopefully, tomorrow we can miniprep the plasmids and send them for sequencing.
