Team:Technion/23 July 2012
From 2012.igem.org
(Difference between revisions)
(Created page with "{{:Team:Technion/Template:Notebook|content= __NOTOC__ ==Ilya== - The ligations and transformations worked. The yield was very small but we still got a few colonies on the plates ...") |
|||
Line 4: | Line 4: | ||
- The ligations and transformations worked. The yield was very small but we still got a few colonies on the plates that were plated with the rest of the cells (after centrifugation).<br> | - The ligations and transformations worked. The yield was very small but we still got a few colonies on the plates that were plated with the rest of the cells (after centrifugation).<br> | ||
- Did colony PCR reactions on 3 colonies from Fus1 HF, 3 colonies from Fus1 GC and 6 colonies of Fus2.<br> | - Did colony PCR reactions on 3 colonies from Fus1 HF, 3 colonies from Fus1 GC and 6 colonies of Fus2.<br> | ||
+ | - Only 1 colony of Fus1 gave positive results and 4 colonies of Fus2 gave positive results.<br> | ||
+ | - Put starters 10 ml each of the only positive colony of Fus1 and two positive colonies of Fus2. I also left the plate with the isolated colonies in the 37 incubator to grow some more. Hopefully, tomorrow we can miniprep the plasmids and send them for sequencing. | ||
==Inbal== | ==Inbal== |
Revision as of 15:22, 23 July 2012
|
|
|
|
|
|
|
|
|
Ilya
- The ligations and transformations worked. The yield was very small but we still got a few colonies on the plates that were plated with the rest of the cells (after centrifugation).
- Did colony PCR reactions on 3 colonies from Fus1 HF, 3 colonies from Fus1 GC and 6 colonies of Fus2.
- Only 1 colony of Fus1 gave positive results and 4 colonies of Fus2 gave positive results.
- Put starters 10 ml each of the only positive colony of Fus1 and two positive colonies of Fus2. I also left the plate with the isolated colonies in the 37 incubator to grow some more. Hopefully, tomorrow we can miniprep the plasmids and send them for sequencing.