Team:Utah State/Results

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<p align="center"><img src="https://static.igem.org/mediawiki/2012/c/c1/Resultssilk.PNG" alt="usu_silk" width="600" height="300"></p>
<p align="center"><img src="https://static.igem.org/mediawiki/2012/c/c1/Resultssilk.PNG" alt="usu_silk" width="600" height="300"></p>
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After cloning separate spider silk units (U, W, F, and B) into pSB1C3 we wanted to show the range of spider silk subunits available to produce different spider silk. We decided to make ‘spider steps’ or ‘spider ladder’ out of the ‘B’ and ‘F’ constructs. These are shown in the agarose gel in the figure below. From this figure there is an insert and a vector band (pSB1C3) and the insert size increases with increased number of repeating subunits. Also, at the high level of repeating unit the insert is larger than the vector backbone. In addition to showing spider silk units, we believe that this is a classic demonstration of the standard assembly which is an excellent tool to teach a new person the methods of standard assembly.
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Revision as of 22:12, 3 October 2012

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