Team:Technion/Attributions
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During our project we have used parts that were given to us by different labs or were taken from the registry/distribution kit. | During our project we have used parts that were given to us by different labs or were taken from the registry/distribution kit. | ||
- | + | ===Donated parts=== | |
- | *''' Ann K. Ganesan''' - WT T7 RNAP | + | * ''' Ann K. Ganesan''' - WT T7 RNAP |
* '''Changwon Kang''' - WT SP6 RNAP | * '''Changwon Kang''' - WT SP6 RNAP | ||
* '''Christopher A. Voight''' - T7*, T7*(T3), T7*(N4), T7*(K1F) RNAPs with suitable induced promoters: pT7, pT3, pK1F, pN4 | * '''Christopher A. Voight''' - T7*, T7*(T3), T7*(N4), T7*(K1F) RNAPs with suitable induced promoters: pT7, pT3, pK1F, pN4 | ||
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* '''Roee's Lab''' (Roee Amit) - Cerulean FP under control of pLac/Ara, mCitrin FP and mCherry under control of pTetO. | * '''Roee's Lab''' (Roee Amit) - Cerulean FP under control of pLac/Ara, mCitrin FP and mCherry under control of pTetO. | ||
- | + | ===Parts from Distribution Kit=== | |
{|border="3" | {|border="3" | ||
|+ | |+ | ||
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|[http://partsregistry.org/Part:pSB3C5 pSB3C5]|| Low to medium copy BioBrick standard vector | |[http://partsregistry.org/Part:pSB3C5 pSB3C5]|| Low to medium copy BioBrick standard vector | ||
|} | |} | ||
+ | ===General=== | ||
==Contributions== | ==Contributions== |
Revision as of 16:03, 26 September 2012
Contents |
Attributions
During our project we have used parts that were given to us by different labs or were taken from the registry/distribution kit.
Donated parts
- Ann K. Ganesan - WT T7 RNAP
- Changwon Kang - WT SP6 RNAP
- Christopher A. Voight - T7*, T7*(T3), T7*(N4), T7*(K1F) RNAPs with suitable induced promoters: pT7, pT3, pK1F, pN4
- Gallivan Lab (Gallivan Justin P., Lynch Sean A.) - Theophylline riboswitch and pTAC
- Roee's Lab (Roee Amit) - Cerulean FP under control of pLac/Ara, mCitrin FP and mCherry under control of pTetO.
Parts from Distribution Kit
Name | Description | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] | Double terminator | [http://partsregistry.org/Part:BBa_F2620 BBa_F2620] | TetR controllable LuxR and lux pR genes | [http://partsregistry.org/Part:BBa_I0462 BBa_I0462] | LuxR protein generator | [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] | GFP under pTetO control | [http://partsregistry.org/Part:BBa_J06702 BBa_J06702] | mCherry, bacterial with RBS and forward terminator | [http://partsregistry.org/Part:BBa_R0062 BBa_R0062] | Promoter (luxR & HSL regulated -- lux pR) | [http://partsregistry.org/Part:pSB1C3 pSB1C3] | High copy BioBrick assembly plasmid | [http://partsregistry.org/Part:pSB3C5 pSB3C5] | Low to medium copy BioBrick standard vector |
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General
Contributions
- We have submitted DNA for 25 BioBricks to the registry. Due to lack of time we haven't managed to put all the parts inside the shipping plasmid.
- We have managed to successfully clone 4 out of 8 lambda phage fragments that the phage's genome has been divided into.
- We have created new reporter system to test our YES gates and hope that it will become standard system for testing other parts as well. We used it to characterize T7 RNAP activity, the results can be seen here.
- We have managed to combine a theophylline riboswitch with T7, T7*(T3), T7*(K1F), T7*(N4) by using fusion PCR under the control of a tac promoter, more details can be seen at RS section. The riboswitch with T7 and T7*(N4) were cloned into the shipping plasmid and submitted as BioBricks. Due to lack of time we hadn't transferred RS+RNAP constructs under pLux control, as was planned at the beginning.
- We have produced a working YES gate #1 with T7* RNAP.
- We have added MCS to pSB1C3 plasmid backbone. This backbone is very convenient to work with, some of our parts had the same restriction sites as standard prefix and suffix found in the backbone, so we couldn't insert them into the pSB1C3 plasmid. Addition of MCS helps to circumvent this problem.
- We entered experience for the following existing BioBricks: [http://partsregistry.org/Part:pSB3C5:Experience pSB3C5] and [http://partsregistry.org/Part:BBa_I0462:Experience BBa_I0462].