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Team:Technion/Project/Phage
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| + | ==Overview== | ||
| + | The main objective of this project is to creat¬¬¬e phage lambda that goes through its lytic cycle only under specific conditions that are met in the bacterial host. The idea is to replace one phage protein location in the genome, under a new regulatory promoter. This will allow the phage lytic cycle only in inducible conditions, controlled by the engineered plasmids that express RNA-polymerases. | ||
| + | This project included planning the genetic manipulation of the phage genome. This includes: | ||
| + | |||
| + | *Phage deviation in to fragments that will ease the genetic manipulation, and re-factoring of the phages genome, after cutting it into fragments. | ||
| + | |||
| + | *Planning of the Q gene deletion and re-insertion under the desirable regulation, the RNA-polymerase promoter. | ||
| + | |||
| + | *The design of the antibiotic resistance gene insertion into the phage genome, in order to create additional selection to bacteria that contain the phage lysogenic genome. | ||
| + | |||
==The chosen phage lambda strain== | ==The chosen phage lambda strain== | ||
Revision as of 09:33, 26 September 2012
- Home
- Team
- Project
- Parts
- Notebook
- Safety
- Attributions & Achievements
- Human Practices
- Sponsors & Thanks
Contents |
Overview
The main objective of this project is to creat¬¬¬e phage lambda that goes through its lytic cycle only under specific conditions that are met in the bacterial host. The idea is to replace one phage protein location in the genome, under a new regulatory promoter. This will allow the phage lytic cycle only in inducible conditions, controlled by the engineered plasmids that express RNA-polymerases. This project included planning the genetic manipulation of the phage genome. This includes:
- Phage deviation in to fragments that will ease the genetic manipulation, and re-factoring of the phages genome, after cutting it into fragments.
- Planning of the Q gene deletion and re-insertion under the desirable regulation, the RNA-polymerase promoter.
- The design of the antibiotic resistance gene insertion into the phage genome, in order to create additional selection to bacteria that contain the phage lysogenic genome.
