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From 2012.igem.org
(Difference between revisions)
(Created page with "http://www.blackwellpublishing.com/genecloning/pdfs/chapter7.pdf") |
(Prokaryotic System, Protein Characterization, and Yeast Genome Integration) |
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- | http://www.blackwellpublishing.com/ | + | '''Yeast System''' <br/> |
+ | <li>[http://www.google.com/url?q=http%3A%2F%2Fbiochemie.web.med.uni-muenchen.de%2FYeast_Biol%2F04%2520Yeast%2520Molecular%2520Techniques.pdf&sa=D&sntz=1&usg=AFQjCNEnj2JbQh6nxUnja5PpZ3Vch5Jy6g Yeast Vectors 1] | ||
+ | <li>[http://www.google.com/url?q=http%3A%2F%2Fwww.blackwellpublishing.com%2Fgenecloning%2Fpdfs%2Fchapter7.pdf&sa=D&sntz=1&usg=AFQjCNEl8DNQGCHr4QH-Rb7zmn6oSAZm2A Yeast Vectors 2] | ||
+ | <li>[http://www.google.com/url?q=http%3A%2F%2Fwww.genetics.org%2Fcontent%2F122%2F1%2F19.full.pdf&sa=D&sntz=1&usg=AFQjCNHIPhHrNJnRxDusytVTLpu59shf0Q A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces ceratisiae]<br/><br/> | ||
+ | |||
+ | '''Yeast Integrative Plasmid Vectors (YIp)'''<br/> | ||
+ | <li>[http://partsregistry.org/Part:BBa_K300001 AL1 and GAL10 (galactose catabolism) selection] | ||
+ | <li>[http://partsregistry.org/Part:BBa_K165061 TRP1 selection]<br/><br/> | ||
+ | |||
+ | '''Prokaryotic System'''<br/><br/> | ||
+ | |||
+ | ''Promoters'' | ||
+ | <li>[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 BBa-J23100 (strong)] | ||
+ | <li>[http://partsregistry.org/Part:BBa_J23108 BBa-J23108 (medium)] | ||
+ | <li>[http://partsregistry.org/Part:BBa_J23109 BBa-J23109 (weak)]<br/> | ||
+ | ''Terminators'' | ||
+ | <li>[http://partsregistry.org/Part:BBa_B0015 BBa-B0015]<br/> | ||
+ | ''RBS'' | ||
+ | <li>[http://partsregistry.org/Part:BBa_B0034 Constitutive]<br/><br/> | ||
+ | |||
+ | '''Secretion''' | ||
+ | <li>[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fgene%2F3990775&sa=D&sntz=1&usg=AFQjCNFRRVh0vsU3zS9AzvSGko6WIQe0Rg HlyA Gene Sequence] | ||
+ | <li>[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F2693460&sa=D&sntz=1&usg=AFQjCNGNhjPJSpqeHsdjbo29PuRXFmWmFA A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium] | ||
+ | <li>[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F1791766&sa=D&sntz=1&usg=AFQjCNFNdr3RpmJXoTQ69ehSmrLMYMRp8Q Analysis of the haemolysin transport process through the secretion from Escherichia coli of PCM, CAT or beta-galactosidase fused to the Hly C-terminal signal domain] | ||
+ | <li>[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F2277639&sa=D&sntz=1&usg=AFQjCNHlr8EyE98_b0wRCRqamhBzJpAw8g Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins]<br/><br/> | ||
+ | |||
+ | '''Kumamolisin Info''' | ||
+ | |||
+ | <li>57 kDa (beta-galactosidase: 540 kDa; [http://www.mpbio.com/detailed_info.php?family_key=02104939] source) | ||
+ | <li>1701 bp | ||
+ | <li>567 aa | ||
+ | <li>MSDMEKPWKEGEEARAVLQGHARAQAPQAVDKGPVAGDERMAVTVVLRRQRAGELAAHVERQAAIAPHAREHLKREAFAASHGASLDDFAELRRFADAHGLALDRANVAAGTAVLSGPVDAINRAFGVELRHFDHPDGSYRSYLGEVTVPASIAPMIEAVLGLDTRPVARPHFRMQRRAEGGFEARSQAAAPTAYTPLDVAQAYQFPEGLDGQGQCIAIIELGGGYDEASLAQYFASLGVPAPQVVSVSVDGASNQPTGDPSGPDGEVELDIEVAGALAPGAKFAVYFAPNTDAGFLDAITTAIHDPTLKPSVVSISWGGPEDSWTSAAIAAMNRAFLDAAALGVTVLAAAGDSGSTDGEQDGLYHVDFPAASPYVLACGGTRLVASGGRIAQETVWNDGPDGGATGGGVSRIFPLPAWQEHANVPPSANPGASSGRGVPDLAGNADPATGYEVVIDGEATVIGGTSAVAPLFAALVARINQKLGKAVGYLNPTLYQLPADVFHDITEGNNDIANRAQIYQAGPGWDPCTGLGSPIGVRLLQALLPSASQPQPGSTENLYFQSGALE |
Revision as of 17:35, 18 June 2012
Yeast System
Yeast Integrative Plasmid Vectors (YIp)
Prokaryotic System
Promoters
Terminators
RBS
Secretion
Kumamolisin Info