Team:Colombia/Parts

From 2012.igem.org

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'''pchS:''' It encode a histidine kinase sensor of a two-component signal transduction system involved in the sensing of the unusual Quorum Sensing signal 3OH-PAME.  
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'''pchS:''' It encode a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.
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'''pchR:''' Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.
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'''pchR:''' Receptor of a two-component signal transduction system involved in the sensing of the unusual Quorum Sensing signal 3OH-PAME. It is an atypical response regulator that has a histidine kinase as its output domain, autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.
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'''pchA:'''LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.
'''pchA:'''LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.
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'''PxpcR:'''
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'''PxpsR:'''Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.
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'''PxpsR-eYFP:'''Fluorescent reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.
== ''Aliivibrio fischeri'' ==
== ''Aliivibrio fischeri'' ==

Revision as of 03:21, 27 September 2012

Template:Https://2012.igem.org/User:Tabima

Contents

Parts

Toxin- Antitoxin (TA) Modules

Our Basic Parts

TA BioBricks1-2.png


Our Composite Parts

TA BioBricks2-2.png

We send all this parts to the Registry!!!!

[http://partsregistry.org/Part:BBa_K831000:Design HipA7] [http://partsregistry.org/Part:BBa_K831003:Design TisB] [http://partsregistry.org/Part:BBa_K831001:Design HipB] [http://partsregistry.org/Part:BBa_K831002:Design MqsA] [http://partsregistry.org/Part:BBa_K831004:Design istR ]

[http://partsregistry.org/Part:BBa_K831015 prm-hipA7] [http://partsregistry.org/Part:BBa_K831009 plac-hipB] [http://partsregistry.org/Part:BBa_K831007 ptetR-mqsR] [http://partsregistry.org/Part:BBa_K831014 plac-istR]

Ralstonia:

Ralstoniaparts.png


pchS: It encode a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.

pchR: Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.

pchA:LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.

PxpsR:Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.

PxpsR-eYFP:Fluorescent reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.

Aliivibrio fischeri

Alis.png

The overall model can be seen in figures in the main page of Aliivibrio/Streptomyces.

chiP: [http://www.ncbi.nlm.nih.gov/gene/3279194 Chitoporin] is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,

chiA: [http://www.ncbi.nlm.nih.gov/gene/3277358 Chitinase] is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.

chiS: [http://www.ncbi.nlm.nih.gov/gene/3279287 The histidine-kinase sensor] is part of a two-component signalling system, that activates a promoter, in this case pChitin.

cbp: [http://www.ncbi.nlm.nih.gov/gene/3279341 Chitin-binding protein] is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.

PChitin: This 100bp region upstream of chiP is thought to be activated by the activation of chiS.

Alert System:

Cutandpasteparts.png