Team:Cambridge/Diary/Week 12
From 2012.igem.org
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===Monday=== | ===Monday=== | ||
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+ | PCR of the biobrick backbone is still proving troublesome, as no bands are coming out. Oli's trying the PCRs at ever higher | ||
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+ | But at least both a restriction digest and a colony PCR confirmed the identity of our Magnesium Riboswitch construct. One of the colonies seems to be fluorescing far more green than the other, which may be down to a mutation that has upregulated our sfGFP. We'll need to do some characterization before we can confirm that, though. | ||
===Tuesday=== | ===Tuesday=== |
Revision as of 15:55, 13 September 2012
Week: | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
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Contents |
Monday
PCR of the biobrick backbone is still proving troublesome, as no bands are coming out. Oli's trying the PCRs at ever higher
But at least both a restriction digest and a colony PCR confirmed the identity of our Magnesium Riboswitch construct. One of the colonies seems to be fluorescing far more green than the other, which may be down to a mutation that has upregulated our sfGFP. We'll need to do some characterization before we can confirm that, though.
Tuesday
Wednesday
Thursday
Friday