Team:Cambridge/Protocols/IPTGInduction

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==IPTG Induction of Ratiometrica in E. coli==
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=IPTG Induction of Ratiometrica in E. coli=
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'''[[Team:Cambridge/Safety/RiskAssessments/IPTGindiction|Risk Assessment]]'''
This protocol is an adaptation of this one: http://www.genetics.wustl.edu/tslab/?page_id=107
This protocol is an adaptation of this one: http://www.genetics.wustl.edu/tslab/?page_id=107
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<center>'''[[Team:Cambridge/Protocols|Back to Protocols]]'''</center>
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Revision as of 13:27, 23 August 2012

IPTG Induction of Ratiometrica in E. coli

Risk Assessment

This protocol is an adaptation of this one: http://www.genetics.wustl.edu/tslab/?page_id=107 and is used to test the induction of the pSPANK promoter with IPTG in E.coli

Preparation

Prepare LB/AMP/IPTG plates with 100ug/ml of Ampicillin and a final concentration of 0.5mM. Also have a couple of normal amp plates ready.

Protocol

1) Pick cultures and grow in 2ml LB/AMP (100ug/ml) in a 15ml snap cap tube overnight at 370C at 160RPM.

2)Dilute 1:100 and grow for 3-4 hours in 2ml LB/AMP (100ug/ml) in a 15ml snap cap tube

3)Spread 250uL and 25uL onto you IPTG plates. Also spread some on normal amp plates as a control. Allow to grow in incubator.


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