Team:Edinburgh/Project/Bioelectric-Interface

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Bio-electric Interface:
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Background
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Part of our project involves focusing on designing, constructing and testing a user-friendly bio-electric interface. Our idea was to create a standardised and regulated electron export system that would work in <i>Escherichia coli</i> as well as other bacteria. As a result, this system would create an output measurable by simple electronic methods, making the whole process easy and reliable. The bio-electric interface would connect the biological and electronic systems and allow integration of biological devices with computers via the generation of electrons. <br>Our ultimate is to achieve a non-toxic, portable and user-friendly device. We've decided to use the molecular electron export machinery genes from the organism <i>Shewanella oneidensis</i> and half fuel cells to measure the change in potential over time, reflecting the export of electrons outside the cell.
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<i>Shewanella oneidensis</i> is one of the organisms known to produce electricity. It gives it an evolutionary advantage over its competitors by allowing <i>S. oneidensis</i> to utilise a multitude of extracellular electron acceptors. Even though the <i>S. oneidensis</i> electron export system is a complicated, multi-component molecular machinery, <a href="#bibliography" onclick="expand('works-cited');">Jensen et al [1]</a> demonstrated that the <b>mtrCAB gene cluster </b> is sufficient to engineer an efficient extracellular electron transport system. As such, we've decided to research and model the system of extracellular electron transport in <i>S. oneidensis</i> and an engineered strain of <i>E. coli</i>. We have identified MtrCAB, CymA and NapC to be elements with the most potential to influence the efficiency of electron export and the <i>ccm</i> cluster to be responsible for cytochrome c maturation [1]. Following these findings, we have modeled the system and attempted to test the selected genes in <i>E coli</i>.
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In both organisms, electrons are being generated by the TCA cycle and need to be transported to a terminal electron acceptor. The first step is the generation of an intracellular quinol pool which serves as an electron shuttle, transporting electrons in the inner membrane of the cell <a href="#bibliography" onclick="expand('works-cited');">[2,3]</a>. The electrons are then transferred to appropriate transmembrane proteins: NapC in <i>E. coli</i> (NapC is a tetraheme cytochrome c quinol dehydrogenase, involved in the nitrate reduction pathway <a href="#bibliography" onclick="expand('works-cited');">[2]</a>) and CymA in <i>S. oneidensis</i> (tetraheme cytochrome c quinol dehydrogenase, related to NapC <a href="#bibliography" onclick="expand('works-cited');">[3]</a>). The transmembrane proteins then interact with MtrA (<i>S. oneidensis</i> decaheme cytochrome c) which is free floating in the periplasm. Reduced MtrA then transfers electrons to MtrC (<i>S. ondeidensis</i> outer membrane decaheme cytochrome c); the MtrA and MtrC interaction  is facilitated by MtrB (<i>S. oneidensis</i> outer membrane beta barrel porin). This way, electrons are transported across the membranes and MtrC can interact with a multitude of electron acceptors outside the cell. <i>S. oneidensis</i> can also utilise nanowires and mediators to further improve the efficiency of the process <a href="#bibliography" onclick="expand('works-cited');">[4]</a>.<br /><br />
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<b>Figure 1:</b> Engineering MtrCAB electron export system in <i>E. coli.</i> a) electron export system in <i>S. oneidensis</i> b) plasmids used by <a href="#bibliography" onclick="expand('works-cited');">Jensen et al.</a> for <i>E coli</i> transformation. C) electron export system in engineered </i>E coli</i> strains (transformed with mtrA or mtrCAB) Jensen H M et al. PNAS 2010;107:19213-19218
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<span class="subtle-emphasis">&lt;&lt;Prev</span> </span></a><span style="color:white;">__</span>1/4</span></a><span style="color:white;">__</span><a href="https://2012.igem.org/Team:Edinburgh/Project/Bioelectric-Interface/Bio-electric-Interface-BioBricks-Cloning"><span class="intense-emphasis">Next&gt;&gt;</span></a></span>
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Latest revision as of 19:50, 26 October 2012

Bio-electric Interface:

Background

Part of our project involves focusing on designing, constructing and testing a user-friendly bio-electric interface. Our idea was to create a standardised and regulated electron export system that would work in Escherichia coli as well as other bacteria. As a result, this system would create an output measurable by simple electronic methods, making the whole process easy and reliable. The bio-electric interface would connect the biological and electronic systems and allow integration of biological devices with computers via the generation of electrons.
Our ultimate is to achieve a non-toxic, portable and user-friendly device. We've decided to use the molecular electron export machinery genes from the organism Shewanella oneidensis and half fuel cells to measure the change in potential over time, reflecting the export of electrons outside the cell.

Shewanella oneidensis is one of the organisms known to produce electricity. It gives it an evolutionary advantage over its competitors by allowing S. oneidensis to utilise a multitude of extracellular electron acceptors. Even though the S. oneidensis electron export system is a complicated, multi-component molecular machinery, Jensen et al [1] demonstrated that the mtrCAB gene cluster is sufficient to engineer an efficient extracellular electron transport system. As such, we've decided to research and model the system of extracellular electron transport in S. oneidensis and an engineered strain of E. coli. We have identified MtrCAB, CymA and NapC to be elements with the most potential to influence the efficiency of electron export and the ccm cluster to be responsible for cytochrome c maturation [1]. Following these findings, we have modeled the system and attempted to test the selected genes in E coli.

In both organisms, electrons are being generated by the TCA cycle and need to be transported to a terminal electron acceptor. The first step is the generation of an intracellular quinol pool which serves as an electron shuttle, transporting electrons in the inner membrane of the cell [2,3]. The electrons are then transferred to appropriate transmembrane proteins: NapC in E. coli (NapC is a tetraheme cytochrome c quinol dehydrogenase, involved in the nitrate reduction pathway [2]) and CymA in S. oneidensis (tetraheme cytochrome c quinol dehydrogenase, related to NapC [3]). The transmembrane proteins then interact with MtrA (S. oneidensis decaheme cytochrome c) which is free floating in the periplasm. Reduced MtrA then transfers electrons to MtrC (S. ondeidensis outer membrane decaheme cytochrome c); the MtrA and MtrC interaction is facilitated by MtrB (S. oneidensis outer membrane beta barrel porin). This way, electrons are transported across the membranes and MtrC can interact with a multitude of electron acceptors outside the cell. S. oneidensis can also utilise nanowires and mediators to further improve the efficiency of the process [4].




Figure 1: Engineering MtrCAB electron export system in E. coli. a) electron export system in S. oneidensis b) plasmids used by Jensen et al. for E coli transformation. C) electron export system in engineered E coli strains (transformed with mtrA or mtrCAB) Jensen H M et al. PNAS 2010;107:19213-19218



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Bibliography (expand)

1. Jensen, H. M., Albers, A. E., Malley, K. R., Londer, Y. Y. , Cohen, B. E., Helms, B. A., Weigele, P., Groves, J. T. & Ajo-Franklin, C. M. (2010). Engineering of a synthetic electron conduit in living cells. PNAS 107, 19213-19218

2. Stewart, V., Lu, Y. & Darwin, A. J. (2002). Periplasmic Nitrate Reductase (NapABC Enzyme) supports Anaerobic Respiration by Escherichia coli K-12. Journal of Bacteriology 184, 1314-1323

3. Marritt, S. J., Lowe, T. G., Bye, J., McMillan, D.G.G., Shi, L., Frederickson, J., Zachara, J., Richardson, D. J., Cheesman, M. R., Jeuken L.J.C. & Butt, J. N. (2012). A functional description of CymA, an electron-transfer hub supporting anaerobic respiratory flexibility in Shewanella. Biochemical Journal 444, 465-474

4. Richter, K., Schicklberger, M., Gescher, J. (2011). Dissimilatory reduction of extracellular electron acceptors in anaerobic respiration. Applied and Environmental Microbiology 78, 913-921

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