Team:UANL Mty-Mexico/Notebook/wetlab
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<p><br><h3>Wetlab</h3><br></p> | <p><br><h3>Wetlab</h3><br></p> | ||
- | <p>Here our most representative genetic | + | <p>Here we present our most representative results, it includes main electrophoresis gels of the more important genetic contructions, as well as the maps of the vectors </p> |
<center> | <center> | ||
<div class="slide1"><img src="https://static.igem.org/mediawiki/2012/7/71/1BifasicGel.jpg" width="600" ></div> | <div class="slide1"><img src="https://static.igem.org/mediawiki/2012/7/71/1BifasicGel.jpg" width="600" ></div> | ||
- | <caption align="bottom"><b>Figure 1.</b>This gel is a partial restriction of the bifasic switch necessary for the HuBac project (iGEM 2011 UANL Mty-Mexico).</caption> | + | <caption align="bottom"><b>Figure 1.</b> This gel is a partial restriction of the bifasic switch necessary for the HuBac project (iGEM 2011 UANL Mty-Mexico).</caption> |
- | <div class="slide2"><img src="https://static.igem.org/mediawiki/2012/d/db/2BiofMap.jpg" width="600" ></div> | + | |
- | <caption align="bottom"><b>Figure 2.</b>.</caption> | + | <div class="slide2"><img src="https://static.igem.org/mediawiki/2012/d/db/2BiofMap.jpg" width="600" ></div> |
- | <div class="slide3"><img src="https://static.igem.org/mediawiki/2012/5/5c/3TubesRedclone.jpg" width="600" ></ | + | <caption align="bottom"><b>Figure 2.</b> Map of the S4-GFP-S1 contruction in the pSB1C3 vector, and a simulation of a digestion with EcoRI and PstI.</caption> |
+ | <br><br> | ||
+ | <div class="slide3"><img src="https://static.igem.org/mediawiki/2012/5/5c/3TubesRedclone.jpg" width="600" ><br><caption align="bottom"><b>Figure 3.</b> Ready for the minipreps! Here are some transformed bacteria cultures, in the right we can see the clone 1 of the 1-3A+L2 genetic construction.</caption> | ||
+ | <br><br> | ||
<div class="slide4"><img src="https://static.igem.org/mediawiki/2012/b/b8/4L2Clone.jpg" width="600"></div> | <div class="slide4"><img src="https://static.igem.org/mediawiki/2012/b/b8/4L2Clone.jpg" width="600"></div> | ||
+ | <caption align="bottom"><b>Figure 4.</b> Here's a partial digest of the construction 1-3A+L2 cutted with XhoI.</caption> | ||
+ | <br><br> | ||
<div class="slide5"><img src="https://static.igem.org/mediawiki/2012/2/24/5aL2Clone.jpg" width="600" ></div> | <div class="slide5"><img src="https://static.igem.org/mediawiki/2012/2/24/5aL2Clone.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 5.</b> Positive restriction digest of 1-3A+L2 and 1-3A (pSB1C3) as control vector.</caption> | ||
+ | <br><br> | ||
<div class="slide6"><img src="https://static.igem.org/mediawiki/2012/6/65/5bL2Clonemap.jpg" width="600" ></div> | <div class="slide6"><img src="https://static.igem.org/mediawiki/2012/6/65/5bL2Clonemap.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 6.</b> Maps and simulation of restriction digest of p1C3-L2 and pSB1C3, simulation with XhoI.</caption> | ||
+ | <br><br> | ||
<div class="slide7"><img src="https://static.igem.org/mediawiki/2012/7/7b/6_600th.jpg" width="600" ></div> | <div class="slide7"><img src="https://static.igem.org/mediawiki/2012/7/7b/6_600th.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 7.</b> 600th, not just a regular gel, 10 tracks of Cph8 (photoreceptor), 2 tracks of 1-23L (terminator), 10 tracks of S1TT (genetic contruction of a the first part Biphasic switch and terminator) and a last track of S4-GFP (second part of the Biphasic swith) all of these used for HuBac project.</caption> | ||
+ | <br><br> | ||
<div class="slide8"><img src="https://static.igem.org/mediawiki/2012/5/5a/7GenScript.jpg" width="600" ></div> | <div class="slide8"><img src="https://static.igem.org/mediawiki/2012/5/5a/7GenScript.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 8.</b> Synthetic genes.</caption> | ||
+ | <br><br> | ||
<div class="slide9"><img src="https://static.igem.org/mediawiki/2012/e/e7/8GenScriptClones.jpg" width="600" ></div> | <div class="slide9"><img src="https://static.igem.org/mediawiki/2012/e/e7/8GenScriptClones.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 9.</b> Cloning the synthetics, minipreps of he synthetics genes.</caption> | ||
+ | <br><br> | ||
<div class="slide10"><img src="https://static.igem.org/mediawiki/2012/d/d0/9p21Clones.jpg" width="600" ></div> | <div class="slide10"><img src="https://static.igem.org/mediawiki/2012/d/d0/9p21Clones.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 10.</b> Subcloning the synthetic constitutive promoter into 1-3A.</caption> | ||
+ | <br><br> | ||
<div class="slide11"><img src="https://static.igem.org/mediawiki/2012/0/09/10p21ClonesMaps.jpg" width="600" ></div> | <div class="slide11"><img src="https://static.igem.org/mediawiki/2012/0/09/10p21ClonesMaps.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 11.</b> Maps and digestions simulations of 1-3A+p21 (constitutive promoter) and 1-3A both cutted with XhoI.</caption> | ||
+ | <br><br> | ||
<div class="slide12"><img src="https://static.igem.org/mediawiki/2012/8/86/11MTL2clones.jpg" width="600" ></div> | <div class="slide12"><img src="https://static.igem.org/mediawiki/2012/8/86/11MTL2clones.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 12.</b> Electrophoresis gels of rhMT clones, rhMT+L2 and 1-3A (rhMT iin pUC57 vector).</caption> | ||
+ | <br><br> | ||
<div class="slide13"><img src="https://static.igem.org/mediawiki/2012/4/47/12MTL2clonesMap.jpg" width="600" ></div> | <div class="slide13"><img src="https://static.igem.org/mediawiki/2012/4/47/12MTL2clonesMap.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 13.</b> Simulation of restriction digest of rhMT in pUC57, digested with EcoRI-PstI.</caption> | ||
+ | <br><br> | ||
<div class="slide14"><img src="https://static.igem.org/mediawiki/2012/d/d6/13MTL2clonesMap.jpg" width="600" ></div> | <div class="slide14"><img src="https://static.igem.org/mediawiki/2012/d/d6/13MTL2clonesMap.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 14.</b> Vector map of L2+rhMT and pSB1C3, simulation of digestion with EcoRI+PstI.</caption> | ||
+ | <br><br> | ||
<div class="slide15"><img src="https://static.igem.org/mediawiki/2012/d/d3/14L2clones.jpg" width="600" ></div> | <div class="slide15"><img src="https://static.igem.org/mediawiki/2012/d/d3/14L2clones.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 15.</b> Electrophoresis gel of different clones of 1-3A+L2 digested with EcoRI+PstI or XhoI, digestion of rhMT+L2 in pSB1C3, cutted with XhoI.</caption> | ||
+ | <br><br> | ||
<div class="slide16"><img src="https://static.igem.org/mediawiki/2012/a/af/15L2clonesMaps.jpg" width="600" ></div> | <div class="slide16"><img src="https://static.igem.org/mediawiki/2012/a/af/15L2clonesMaps.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 16.</b> Simulation of rhMT+L2 in pSB1C3 cutted with EcoRI+PstI or XhoI and p1C3+L2 digested with XhoI.</caption> | ||
+ | <br><br> | ||
<div class="slide17"><img src="https://static.igem.org/mediawiki/2012/8/82/16L2MTclones.jpg" width="600" ></div> | <div class="slide17"><img src="https://static.igem.org/mediawiki/2012/8/82/16L2MTclones.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 17.</b> L2 in pSB1C3 vector digestion assay.</caption> | ||
+ | <br><br> | ||
<div class="slide18"><img src="https://static.igem.org/mediawiki/2012/1/19/17L2MTclonesMaps.jpg" width="600" ></div> | <div class="slide18"><img src="https://static.igem.org/mediawiki/2012/1/19/17L2MTclonesMaps.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 18.</b> Different rhMT+L2 and L2 in p1C3 vector digestion profiles.</caption> | ||
+ | <br><br></div> | ||
<div class="slide19"><img src="https://static.igem.org/mediawiki/2012/8/80/18RedWhite.jpg" width="600" ></div> | <div class="slide19"><img src="https://static.igem.org/mediawiki/2012/8/80/18RedWhite.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 19.</b> GlpF transportator cloned in pSB3T5 bacteria (white colonies).</caption> | ||
+ | <br><br> | ||
<div class="slide20"><img src="https://static.igem.org/mediawiki/2012/5/5d/19GlpF.jpg" width="600" ></div> | <div class="slide20"><img src="https://static.igem.org/mediawiki/2012/5/5d/19GlpF.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 20.</b> GlpF transportator cloned in pSB3T5 digestion</caption> | ||
+ | <br><br> | ||
<div class="slide21"><img src="https://static.igem.org/mediawiki/2012/d/d5/21MicroWave.jpg" width="600" ></div> | <div class="slide21"><img src="https://static.igem.org/mediawiki/2012/d/d5/21MicroWave.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 21.</b> High-speed double digestion in a microwave at different seconds (tracks 2-4). </caption> | ||
+ | <br><br> | ||
<div class="slide22"><img src="https://static.igem.org/mediawiki/2012/f/f2/22_p1C3map.jpg" width="600" ></div> | <div class="slide22"><img src="https://static.igem.org/mediawiki/2012/f/f2/22_p1C3map.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 22.</b> Map of pSB1C3-RFP, and a simulation of a digestion with EcoRI and PstI.</caption> | ||
+ | <br><br> | ||
<div class="slide23"><img src="https://static.igem.org/mediawiki/2012/8/83/23Last.jpg" width="600" ></div> | <div class="slide23"><img src="https://static.igem.org/mediawiki/2012/8/83/23Last.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 23.</b> A little fragment in an electrophoresis gel, aaaand it's gone.</caption> | ||
+ | <br><br> | ||
<div class="slide24"><img src="https://static.igem.org/mediawiki/2012/c/c1/24Doble.jpg" width="600" ></div> | <div class="slide24"><img src="https://static.igem.org/mediawiki/2012/c/c1/24Doble.jpg" width="600" ></div> | ||
+ | <caption align="bottom"><b>Figure 24.</b> As you can see, this is what we mean when we say "work optimization".</caption> | ||
</center> | </center> | ||
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<div class="sidebar2"> | <div class="sidebar2"> | ||
<ul class="nav"> | <ul class="nav"> | ||
- | <a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook"><li> | + | <a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook"><li>Cloning Strategy</a></li> |
<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/wetlab"><li><b>>>Wetlab</b></a></li> | <a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/wetlab"><li><b>>>Wetlab</b></a></li> | ||
<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/protocols"><li>Protocols</a></li> | <a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/protocols"><li>Protocols</a></li> |
Latest revision as of 04:03, 27 September 2012
Wetlab
Here we present our most representative results, it includes main electrophoresis gels of the more important genetic contructions, as well as the maps of the vectors